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  • 1
    Electronic Resource
    Electronic Resource
    A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 141-144 
    Details
    ISSN: 1420-9071
    Keywords: Superoxide ; free radical ; reactive oxygen species ; chemiluminescence ; pancreatitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer, and lucignin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8±337.7 counts/10 sec) were not different from those of females (335±308.7 counts/10 sec) (p=0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522±2014 counts/10 sec) were significantly higher than those of healthy controls (p〈0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923359
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Reduction of calcium excretion in the stone-forming kidney in unilateral ureteral obstruction (1991)
    Springer
    Urological research 19 (1991), S. 193-198 
    Details
    ISSN: 1434-0879
    Keywords: Urolthiasis ; Obstructive uropathy ; Percutaneous nephrostomy ; Hypercalciuria ; Creatinine clearance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Thirteen urolithiasis patients with unilateral obstructive uropathy were treated with percutaneous nephrostomy (PCN) either for urinary diversion, endopyelotomy, nephrolithtotmy or chemolysis. After percutaneous nephrostomy, the indvidual urine volume, creatinine clearance (Ccr), urinary absolute and fractional excretions of sodium, potassium, calcium, magnesium and inorganic phosphate were measured separately in timed urine collections from a pigtail catheter and from the urethra. The data showed that Ccr and the absolute urinary excretions of sodium, potassium, calcium, magnesium and inorganic phosphate were significantly lower in the PCN kidney immediately or 2 days after relief of obstruction. The ratio of total urinary calcium excretion to urinary creatinine excretion in the obstructed kidney was significantly greater than that in the contralateral kidney. The fractional excretions of calcium and magnesium increased as renal function decreased. The results showed that when the total Ccr is below normal, the apparent excretion of urinary calcium will be underestimated. However, when the total Ccr of patients is within normal range, hypercalciuria may be detected adequately and thus favors early implementation of an appropriate therapeutic strategy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303749
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Menadione-induced cytotoxicity to rat osteoblasts (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 967-976 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Menadione; oxidative stress; osteoblasts; superoxide dismutase (SOD); catalase.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Oxygen-derived free radical injury has been associated with several cytopathic conditions. Oxygen radicals produced by chondrocytes is an important mechanism by which chondrocytes induce matrix degradation. In the present study, we extend these observations by studying oxidative processes against osteoblasts. Osteoblasts were mixed in in vitro culture with 200 μM menadione. The cytotoxic effect of menadione-induced oxidative stress was monitored by lucigenin- or luminol-amplified chemiluminescence, tetrazolium assay and immunocytochemical study. Results showed that adding menadione induces an oxidative stress on osteoblasts, via superoxide and hydrogen peroxide production, that can be eradicated by superoxide dismutase (SOD) and catalase in a dose-dependent manner. Catalase and the appropriate concentration of dimethyl sulfoxide have a protective effect on cytotoxicity induced by menadione, whereas SOD does not. Menadione-treated osteoblasts have a strong affinity for annexin V, and the nuclei are strongly stained by TUNEL (TdT-mediated dUTP nick-end labelling). The results suggest that menadione-triggered production of reactive oxygen species leads to apoptosis of osteoblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050118
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  • 4
    Electronic Resource
    Electronic Resource
    Early third-stage larvae of Gnathostoma hispidum in cyclops (1987)
    Springer
    Parasitology research 74 (1987), S. 69-72 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Surface structures of the early third-stage larae were examined by scanning electron microscopy. There were hemispherical head-bulbs at the apical ends. These bulbs could be clearly distinguished from the bodies. The head-bulbs (52 × 29 Μm) had four transverse rows of sharp booklets. The number of booklets in each row was 37, 36, 38, and 43, posteriorly. The larvae possessed a pair of lateral lips in the head-bulb. Each of the lips had two labial papillae and an amphid between the papillae. Small unidentate cuticular spines were present along the entire length of larvae on their transverse striations. The number of these cuticular striations was 175–217. A pair of cervical papillae and an excretory pore were present on the anterior part of the body. Another pair of papillae was detected laterally on the posterior one-third of the body. The shape of the posterior body papillae resembled that of cervical papillae. The cuticular spines were absent around the anus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00534935
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    Paper (German National Licenses)
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