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  • Springer  (3)
Document type
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  • 1
    Electronic Resource
    Electronic Resource
    On-line measurements of culture fluorescence: Method and application (1981)
    Springer
    Applied microbiology and biotechnology 13 (1981), S. 10-14 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In this paper a new probe allowing the measurement of NAD(P)H-dependent culture fluorescence in a bioreactor is presented. This sterilizable probe can be inserted in every bioreactor using a standard fitting of 25 mm. Under well defined conditions high specificity and sensitivity as well as high stability are further advantages of this probe. Application examples are given to demonstrate the operation possibilities of this fluorescence probe. In batch growth the culture fluorescence can be used for on-line estimation of biomass concentration. Metabolic alterations due to substrate of oxygen deficiency can easily be detected by fluorometric measurements. In kinetic studies the fluorescence probe is of great use because of a very small time delay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00505334
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Design and characterization of a completely filled stirred bioreactor (1980)
    Springer
    Applied microbiology and biotechnology 9 (1980), S. 83-91 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A newly designed bioreactor which is operated completely filled with a mixture of liquid and entrained gas has been compared with a classical stirred system. The completely filled stirred bioreactor (CFSB) consists essentially of a marine type propeller installed in a draft tube and a gas-liquid separator at the top of the vessel. The reactors have been compared on the basis of oxygen transfer rates, mixing times, power input, batch and chemostat experiments with yeasts and mycelial molds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503503
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Respiratory activity of Candida tropicalis during growth on hexadecane and on glucose (1972)
    Springer
    Archives of microbiology 82 (1972), S. 247-253 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rates of oxygen uptake and the oxygen demand during growth of Candida tropicalis on hexadecane and glucose were determined in batch experiments. Oxygen demand was 2.5 fold higher for the synthesis of one unit of cell mass from hydrocarbon than from glucose. On the other hand specific respiration is of the same order of magnitude for both substrates, e.g. 12 mmoles O2xh-1xg-1 (dry weight) and seems to be a constant of this organism. Higher rates of oxygen supply into the medium had no effect on the specific rates of respiration. Specific growth rates on hexadecane were 2.4 times lower than on glucose. It is concluded, that rates of synthesis of cell components are controlled by the overall capacity of the respiratory pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412196
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    Paper (German National Licenses)
    Fulltext
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