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  • 1
    Electronic Resource
    Electronic Resource
    Investigation of the low lying levels in9B (1974)
    Springer
    The European physical journal 267 (1974), S. 331-336 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The reaction10B(3He, α)9B(p)8Be(g.s.) has been studied using a 1.8 MeV3He+ beam. Coincidence spectra were measured at θα=−150
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01669963
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  • 2
    Electronic Resource
    Electronic Resource
    Absence of FMR-1 gene expression can be detected with RNA extracted from dried blood specimens (1994)
    Springer
    Human genetics 〈Berlin〉 93 (1994), S. 488-493 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fragile X syndrome is a genetic disorder caused by abnormal function of the FMR-1 gene. The majority of fragile X syndrome patients carry an expansion of the CGG tri-nucleotide repeat in the FMR-1 gene, whereas others have a deletion or a point mutation in the FMR-1 structural gene. In this report, we analyzed a typical family with three male patients. RNA from Epstein-Barr virus transformed lymphoblastoid cells was used for RNase protection assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Five normal individuals and one asymptomatic heterozygote from this family expressed detectable FMR-1 transcripts, whereas three fragile X patients showed no sign of expression with either assay. To extend the application of this PCR-based assay to laboratory diagnosis of fragile X syndrome, we confirmed that dried blood samples collected on screening filter papers for newborns are an adequate source of RNA for RTPCR. Moreover, fragile X patients from the study family and another family were reliably identified by the absence of the FMR-1-specific PCR product from the dried blood specimens. Our studies indicate that this simple assay can be used to diagnose the fragile X syndrome for the majority of male patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202810
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  • 3
    Electronic Resource
    Electronic Resource
    Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacterium tumefaciens (1996)
    Springer
    Plant cell reports 15 (1996), S. 653-657 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile transgenic plants of peanut (Arachis hypogaea L. cv. New Mexico Valencia A) were produced using an Agrobacterium-mediated transformation system. Leaf section explants were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBI121 containing the genes for β-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII). Approximately 10% of the shoots regenerated on selection medium were GUS-positive. Five independent transformation events resulted in the production of 52 fertile transgenic peanut plants. On average, 240 d were required between seed germination for explant preparation and the production of mature t1 seed by T0 plants. Molecular analysis of transgenic plants confirmed the stable integration of the transgenes into the peanut genome. GUS expression segregated in a 3∶1 Mendelian ratio in most T1 generation plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231918
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  • 4
    Electronic Resource
    Electronic Resource
    Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacterium tumefaciens (1996)
    Springer
    Plant cell reports 15 (1996), S. 653-657 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: GUS, β-glucuronidase; NPTII, neomycin-phosphotransferase II; MS medium, Murashige and Skoog medium (1962); BA, N6-benzyladenine, NAA, 1-naphthaleneacetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Fertile transgenic plants of peanut (Arachis hypogaea L. cv. New Mexico Valencia A) were produced using an Agrobacterium-mediated transformation system. Leaf section explants were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBI121 containing the genes for β-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII). Approximately 10% of the shoots regenerated on selection medium were GUS-positive. Five independent transformation events resulted in the production of 52 fertile transgenic peanut plants. On average, 240 d were required between seed germination for explant preparation and the production of mature T1 seed by T0 plants. Molecular analysis of transgenic plants confirmed the stable integration of the transgenes into the peanut genome. GUS expression segregated in a 3:1 Mendelian ratio in most T1 generation plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050092
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of polyamine and S-adenosylmethionine contents of growing and encysted Acanthamoeba isolates (1989)
    Springer
    Molecular and cellular biochemistry 90 (1989), S. 145-153 
    Details
    ISSN: 1573-4919
    Keywords: Acanthamoeba ; polyamines ; S-adenosylmethionine ; amoebae ; cysts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have used High Performance Liquid Chromatography to determine metabolite characteristics of three recent isolates of Acanthamoeba which exhibit cultural characteristics consistent with those of established potential pathogens. Growing amoebae and dormant cysts of these isolates were explored in regard to their qualitative and quantitative intracellular levels of polyamine and S-adenosylmethionine metabolites. The polyamine found in the greatest concentration in the growing cells was 1,3-diaminopropane (DAP), followed by spermidine (SPD). A low level of putrescine was also found in the growing cells. These polyamines significantly decreased in concentration as the amoebae differentiated to cysts. N8-acetylspermidine and acetylspermine were found in both developmental stages while acetylcadaverine was found only in growing amoebae and N1-acetylspermidine only in cysts. Acetylputrescine was present in both stages of two isolates but only in the growing amoebae of the third isolate. Spermine was not detected in any of the isolates. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) were present in growing amoebae but SAM was undetectable or barely detectable in cysts. SAH also decreased in concentration during encystation of two of the isolates to a level comparable to that of the other isolate. The developmental transition from growing amoebae to dormant cysts is characterized metabolically by a threshold adjustment in concentration of SAM, SAH and of the polyamines (esp., DAP and SPD).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221214
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  • 6
    Electronic Resource
    Electronic Resource
    Development of monoclonal antibodies against bovine mucin core 2 β6 N-acetylglucosaminyltransferase (1999)
    Springer
    Glycoconjugate journal 16 (1999), S. 555-562 
    Details
    ISSN: 1573-4986
    Keywords: core 2 N-acetylglucosaminyltransferase ; glycosyltransferase ; monoclonal antibodies ; mucin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Molecular cloning techniques have been used to produce abundant amounts of recombinant glycosyltransferases for biochemical studies. We recently cloned a cDNA which encoded bovine mucin core 2 β6N-acetylglucosaminyl transferase (C2TF). Poly-histidine-C2TF fusion protein was generated from the cloned cDNA in the E. coli Xpress system and used to produce monoclonal antibodies (MAbs). We obtained seven hybridomas which secreted MAbs against bovine C2TF in mouse ascites with titers ranging from 1:1280 to 1:40960 as assessed by immunofluorescence assay (IF). Isotyping revealed that all seven MAbs were IgG (4 IgG1, 2 IgG2b and 1 IgG2a). The affinity constants (M−2) for these MAbs range from 5.4 × 107 to 1.2 × 109. These MAbs recognized bovine C2TF in tissue sections and on Western blottings. Six of these MAbs reacted with human core 2-M enzyme and one with both core 2-L and core 2-M enzymes on Western blottings. Therefore, These antibodies should be useful for further study of bovine and human core 2 enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007030223118
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  • 7
    Electronic Resource
    Electronic Resource
    Kinetics of the sequential dechlorination of 2,4,6-trichlorophenol by an anaerobic microbial population (1995)
    Springer
    Biotechnology letters 17 (1995), S. 663-668 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A mathematical model was developed to describe the sequential dechlorination of 2,4,6-trichlorophenol to 2,4-dichlorophenol, 4-chlorophenol and phenol. Each compound was assumed to be degraded according a Michaelis-Menten expression. Experimental data were used to obtain the model kinetic constants and to test its validity. Good agreement between the model predictions and the experimental data was obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00129397
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  • 8
    Electronic Resource
    Electronic Resource
    On the shrinkage of local linear curve estimators (1997)
    Springer
    Statistics and computing 7 (1997), S. 11-17 
    Details
    ISSN: 1573-1375
    Keywords: Bandwidth ; bias ; compactly supported kernel ; kernel estimator ; mean squared error ; ridge parameter ; smoothing ; variance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract Local linear curve estimators are typically constructed using a compactly supported kernel, which minimizes edge effects and (in the case of the Epanechnikov kernel) optimizes asymptotic performance in a mean square sense. The use of compactly supported kernels can produce numerical problems, however. A common remedy is ‘ridging’, which may be viewed as shrinkage of the local linear estimator towards the origin. In this paper we propose a general form of shrinkage, and suggest that, in practice, shrinkage be towards a proper curve estimator. For the latter we propose a local linear estimator based on an infinitely supported kernel. This approach is resistant against selection of too large a shrinkage parameter, which can impair performance when shrinkage is towards the origin. It also removes problems of numerical instability resulting from using a compactly supported kernel, and enjoys very good mean squared error properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018549127542
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  • 9
    Electronic Resource
    Electronic Resource
    Dehalogenation and mineralization of 2,4,6-trichlorophenol by the sequential activity of anaerobic and aerobic microbial populations (1992)
    Springer
    Biotechnology letters 14 (1992), S. 143-148 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A sequential anaerobic-aerobic treatment process that can mineralize 2,4,6-trichlorophenol has been developed. The process uses diluted anaerobic digester fluid as a culture medium, and a single microbial population enriched from the digester fluid for both the anaerobic and aerobic steps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01026242
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  • 10
    Electronic Resource
    Electronic Resource
    Recovery and purification of thuringiensin from the fermentation broth of Bacillus thuringiensis (1998)
    Springer
    Bioseparation 7 (1998), S. 309-316 
    Details
    ISSN: 1573-8272
    Keywords: Bacillus thuringiensis ; capillary electrophoresis ; cetylpyridinium chloride ; micellar-enhanced ultrafiltration ; thuringiensin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Thuringiensin is a heat stable β-exotoxin from Bacillus thuringiensis with a great potential for replacing the traditional chemical pesticides. A process using micellar-enhanced ultrafitration method to recover thuringiensin was significantly improved by the use of a spiral-wound membrane, which could be operated at a low transmembrane pressure drop. This method was performed by adding a surfactant cetylpyridinium chloride (CPC) into the fermentation broth. After the surfactant-thuringiensin conjugates were formed, the broth then passed through the ultrafiltration membrane and the retentate was collected. The results indicated the optimal concentration of CPC for producing a maximal recovery up to 99.3% is 4%. For purification, the centrifuged broth was further filtered by a membrane filter. The filtered solution then was mixed with 50% of activated carbon. The supernatant then was injected into a preparative HPLC. The eluate was collected during thuringiensin peak formation. This eluate was then concentrated by vacuum evaporation and dialysis using an electrodialyzer to remove the excess salts. The dialyzed solution was then crystallized by lyophilization. The purity of the thuringiensin crystal was identified by HPLC, capillary electrophoresis, and mass spectrometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008128717110
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