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  • 1
    Electronic Resource
    Electronic Resource
    Maize DNA enrichment by representational difference analysis in a maize chromosome addition line of oat (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 337-344 
    Details
    ISSN: 1432-2242
    Keywords: Key words Representational difference analysis(RDA) ; Maize ; Oat ; Addition line ; Repetitive DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The recent recovery of maize (Zea mays L.) single-chromosome addition lines of oat (Avena sativa L.) from oat x maize crosses has provided novel source materials for the potential isolation of maize chromosome-specific sequences for use in genetic mapping and gene cloning. We report here the application of a technique, known as representational difference analysis (RDA), to selectively isolate maize sequences from a maize chromosome-3 addition line of oat. DNA fragments from the addition line and from the oat parent were prepared using BamHI digestion and primer ligation followed by PCR amplification. A subtractive hybridization technique using an excess of the oat parental DNA was employed to reduce the availability for amplification of DNA fragments from the addition lines that were in common with the ones from the oat parental line. After three rounds of hybridization and amplification, the resulting DNA fragments were cloned into a plasmid vector. A DNA library containing 400 clones was constructed by this method. In a test of 18 clones selected at random from this library, four (22%) detected maize-specific repetitive DNA sequences and nine (50%) showed strong hybridization to genomic DNA of maize but weak hybridization to genomic DNA of oat. Among these latter nine clones, three detected low-copy DNA sequences and two of them detected DNA sequences specific to chromosome 3 of maize, the chromosome retained in the source maize addition line of oat. The other eight out of the 13 clones that had strong hybridization to maize DNA detected repetitive DNA sequences or high-copy number sequences present on most or all maize chromosomes. We estimate that the maize DNA sequences were enriched from about 1.8% to over 72% of the total DNA by this procedure. Most of the isolated DNA fragments detected multiple or repeated DNA sequences in maize, indicating that the major part of the maize genome consists of repetitive DNA sequences that do not cross-hybridize to oat genomic sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050904
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  • 2
    Electronic Resource
    Electronic Resource
    Authors' reply (1996)
    Springer
    European journal of plastic surgery 19 (1996), S. 172-172 
    Details
    ISSN: 1435-0130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00176273
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of glutathione, superoxide dismutase and adenosine triphosphate on venous flap survival (1996)
    Springer
    European journal of plastic surgery 19 (1996), S. 170-173 
    Details
    ISSN: 1435-0130
    Keywords: Venous flaps ; GSH ; SOD ; ATP-MgCl2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Oxygen-derived free radicals and the energy supply for resynthesis are important factors that affect flap survival. An experimental rabbit model was designed to investigate the effects of the free radical scavengers, glutathione (GSH), superoxide dismutase (SOD), and adenosine triphosphate (ATP-MgCL2), on venous flap survival. Four 4×6 cm flaps were elevated in each animal along the axis of the thoraco-epigastric veins. Postoperatively, the agents were administered intravenously and relative flap survival was measured after two weeks. Mean flap survival, using the free radical scavengers, ranged between 76 and 87%, which showed a significant improvement over saline controls (68.73%, p〈0.01). The respective mechanisms of the individual agents are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00176271
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  • 4
    Electronic Resource
    Electronic Resource
    Laser influence on positron-antiproton radiative capture collision (1999)
    Springer
    The European physical journal 7 (1999), S. 39-44 
    Details
    ISSN: 1434-6079
    Keywords: PACS. 34.50.Rk Laser-modified scattering and reactions - 34.70.+e Charge transfer - 32.80.Wr Other multiphoton processes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: The laser-assisted radiative capture between a positron and an antiproton is studied in detail. The theoretical results show that the cross-section for antihydrogen formation is significantly reduced with the application of a laser background. This effect is most marked when the laser polarization is parallelto the incident direction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100530050345
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