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  • Quantitative J correlation  (1)
  • φ angle  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    χ1 angle information from a simple two-dimensional NMR experiment that identifies trans 3JNCγ couplings in isotopically enriched proteins (1997)
    Springer
    Journal of biomolecular NMR 9 (1997), S. 323-328 
    Details
    ISSN: 1573-5001
    Keywords: χ1 angle ; Calmodulin ; Carbon-nitrogen J coupling ; Quantitative J correlation ; Stereospecific assignment ; Ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract New quantitative J correlation experiments are used for measuring all two- and three-bondcouplings between 15N and aliphatic side-chain carbons in proteins uniformly enriched in 13Cand 15N. Results show that 3JNCβ and 2JNCβ invariably are very small.Therefore, a simple and relatively sensitive two-dimensional spin-echo difference experimentcan be used to identify residues with a 3JNCγ coupling substantially larger than 1Hz, indicative of a trans arrangement between N and Cγ. This measurement thereforeprovides χ1 angle information for residues with an aliphatic Cγ carbon, andthereby also aids in making stereospecific assignments of Hβ resonances. Experimentsare demonstrated for ubiquitin and for a complex between calmodulin and a 26-residuepeptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018691228238
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Measurement of Three-bond, 13C′-13Cβ J Couplings in Human Ubiquitin by a Triple Resonance, E. COSY-type NMR Technique (1998)
    Springer
    Journal of biomolecular NMR 11 (1998), S. 199-203 
    Details
    ISSN: 1573-5001
    Keywords: carbon-carbon J coupling ; E. COSY ; Karplus equation ; φ angle ; 3D NMR ; ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A [CO]HN(CA)CB-E.COSY pulse scheme is described for measurement of three-bond couplings, 3JC′Cβ, between carbonyl and aliphatic Cβ carbons in ubiquitin, uniformly enriched with 13C and 15N. A Karplus relation, 3JC′Cβ = 1.28 cos2(φ - 120°) -1.02 cos(φ - 120°) +0.30 Hz, is obtained by correlating the 3JC′Cβ values measured for human ubiquitin with backbone φ angles from its crystal structure. As predicted, the new Karplus parametrization yields 3JC′Cβ values slightly larger than previously obtained by quantitative J correlation [Hu, J.-S. and Bax, A. (1997) J. Am. Chem. Soc., 119, 6360-6368], but considerably smaller than what has been reported on the basis of other E.COSY-type measurements carried out on flavodoxin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008241904271
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    Paper (German National Licenses)
    Fulltext
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