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  • CHO  (1)
  • Chlorobium thiosulfatophilum  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Mechanisms of CO2 fixation in bacterial photosynthesis studied by the carbon isotope fractionation technique (1977)
    Springer
    Archives of microbiology 112 (1977), S. 35-38 
    Details
    ISSN: 1432-072X
    Keywords: Photosynthetic bacteria ; Carbon isotope ratio ; CO2 fixation ; Chlorobium thiosulfatophilum ; Rhodospirillum rubrum ; Chlamydomonas reinhardii ; Chromatium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. The carbon isotope discrimination properties of a representative of each of the three types of photosynthetic bacteria Chlorobium thiosulfatophilum, Rhodospirillum rubrum and Chromatium and of the C3-alga Chlamydomonas reinhardii were determined by measuring the ratio of 13CO2 to 12CO2 incorporated during photoautotrophic growth. 2. Chromatium and R. rubrum had isotope selection properties similar to those of C3-plants, whereas Chlorobium was significantly different. 3. The results suggest that Chromatium and R. rubrum assimilate CO2 mainly via ribulose 1,5-diphosphate carboxylase and the associated reactions of the reductive pentose phosphate cycle, whereas Chlorobium utilizes other mechanisms. Such mechanisms would include the ferredoxin-linked carboxylation enzymes and associated reactions of the reductive carboxylic acid cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446651
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The crystal violet nuclei staining technique leads to anomalous results in monitoring mammalian cell cultures (1996)
    Springer
    Cytotechnology 21 (1996), S. 73-80 
    Details
    ISSN: 1573-0778
    Keywords: cell counting ; CHO ; crystal violet ; hybridoma ; trypan blue ; Vero-cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Nuclear counts determined by crystal violet staining from samples of stationary or microcarrier cultures of hybridomas, CHO or Vero cells were consistently and significantly higher than cell concentrations determined by the trypan blue or Coulter counter methods. This difference was attributed to the presence of a significant proportion of binucleated cells, which are assumed to be 35% of the cell population in the stationary phase of Vero cultures. The proportion of such cells during exponential growth was variable. However, continuous sub-culture of these cells induced a degree of synchrony during growth which resulted in a cyclic variation of the difference between the cell and nuclei counting techniques. This data indicates that care should be taken in interpreting cell culture profiles based solely on crystal violet nuclei staining counts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364838
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    Paper (German National Licenses)
    Fulltext
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