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  • Biochemical preparation of sulfonucleotides  (1)
  • Ectothiorhodospira halochloris  (1)
  • Fatty acid composition  (1)
  • Springer  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Influence of salt concentration and temperature on the fatty acid compositions of Ectothiorhodospira and other halophilic phototrophic purple bacteria (1991)
    Springer
    Archives of microbiology 156 (1991), S. 370-375 
    Details
    ISSN: 1432-072X
    Keywords: Phototrophic purple bacteria ; Ectothiorhodospira ; Fatty acid composition ; Salt adaptation ; Membrane fluidity ; Bilayer stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Influences of the salt concentration on the fatty acid composition of Ectothiorhodospira species and other phototrophic purple bacteria have been analysed. Major fatty acids in bacteria of the genera Rhodobacter, Rhodopseudomonas, Chromatium, and Ectothiorhodospira were straight chain saturated and monounsaturated C-16 and C-18 fatty acids. Salt-dependent responses of all investigated bacteria revealed relations to their salt optima. Minimum values of C-16 and saturated fatty acids and maximum values of C-18 and unsaturated fatty acids were found at or close to the salt optima. Responses of Ectothiorhodospira mobilis upon changes in salinity were nearly identical, whether cells were grown in batch culture or in continuous culture with identical dilution rates at all salt concentrations. With increasing temperature, the fatty acid composition of Ectothiorhodospira mobilis and Ectothiorhodospira halophila strains showed decreasing portions of C-18 and of unsaturated fatty acids, while the contents of C-16 and saturated fatty acids increased. The results are discussed with respect to bilayer stabilisation and membrane fluidity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248713
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ectothiorhodospira halochloris sp. nov., a new extremely halophilic phototrophic bacterium containing bacteriochlorophyll b (1977)
    Springer
    Archives of microbiology 114 (1977), S. 115-121 
    Details
    ISSN: 1432-072X
    Keywords: Anaerobic phototrophic bacteria ; Ectothiorhodospira halochloris ; Halophilic bacteria ; Alkaliphilic bacteria ; Sulfide oxidation ; Bacteriochlorophyll b
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new bacteriochlorophyll b containing phototrophic bacterium was isolated from extremely saline and alkaline soda lakes in Egypt. Enrichment and isolation were performed using a synthetic medium with high contents of sodium carbonate, sodium sulfate and sodium chloride. Photoautotrophic growth occurred with hydrogen sulfide as photosynthetic electron donor. During oxidation of sulfide to sulfate extracellular elemental sulfur globules appeared in the medium. Cells were also capable to grow under photoheterotrophic conditions with acetate, propionate, pyruvate, succinate, fumarate or malate as carbon sources and electron donors. Under these conditions sulfate was assimilated. Optimal growth under the applied experimental conditions occurred at a total salinity of 14–27%, a pH-range between 8.1 and 9.1 and a temperature between 47°C and 50°C. The cells were 0.5–0.6 μm wide and, depending on cultural conditions, 2.5–8.0 μm long; they were spiral shaped, multiplied by binary fission and were motile by means of bipolar flagella. Intercytoplasmic photosynthetic membranes were present as stacks. Bacteriochlorophyll b was the main photosynthetic pigment; small amounts of carotenoids were mainly present as glucosides of rhodopin and its methoxy derivative. The new organism is described as Ectothiorhodospira halochloris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410772
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Occurrence and evolutionary significance of two sulfate assimilation pathways in the rhodospirillaceae (1982)
    Springer
    Archives of microbiology 132 (1982), S. 197-203 
    Details
    ISSN: 1432-072X
    Keywords: Rhodopseudomonas ; Rhodospirillum ; Rhodomicrobium ; Rhodocyclus ; Sulfate assimilation ; Sulfonucleotide specifity ; Biochemical preparation of sulfonucleotides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ability to use adenosine 5′-phosphosulfate (APS) or 3′-phosphoadenosine 5′-phosphosulfate (PAPS) as the substrate for the initial reductive step in sulfate assimilation has been tested in most of the known Rhodospirillaceae species and in some chemotrophic bacteria. Improved and optimized methods for the synthesis and purification of the sulfonucleotides APS and PAPS are described. The production of acid volatile radioactivity from 35S-APS and 35S-PAPS was measured under various conditions in the presence and absence of non-labeled sulfate. Specific differences in the ability to reduce APS or PAPS were observed among the Rhodospirillaceae species and also the chemotrophic bacteria. APS was found to be the substrate of the thiolsulfotransferase in Rps. acidophila, Rps. globiformis, Rm. vannielii, Rc. purpureus, R. tenue, Rps. gelatinosa, in Alcaligenes eutrophus and Pseudomonas aeruginosa. PAPS was the substrate in Rps. capsulata, Rps. sphaeroides, Rps. sulfidophila, Rps. palustris, Rps. viridis, R. rubrum, R. fulvum, in Paracoccus denitrificans and in several Enterobacteriaceae. The presence of different enzymatic systems for sulfate reduction in the Rhodospirillaceae family is compared with their taxonomical grouping and their possible phylogenetic relatedness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508731
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    Paper (German National Licenses)
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