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  • Arabidopsis thaliana  (2)
  • Environment
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Two different Em-like genes are expressed in Arabidopsis thaliana seeds during maturation (1993)
    Springer
    Molecular genetics and genomics 238 (1993), S. 409-418 
    Details
    ISSN: 1617-4623
    Keywords: Dry seed ; Em-like genes ; Arabidopsis thaliana ; Abscisic acid ; Responsive genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using a radish cDNA probe, we have isolated and characterized two genomic clones from Arabidopsis thaliana (GEA1 and GEA6) encoding two different proteins that are homologous to the “Early methionine-labelled” (Em) protein of wheat. GEA1 differs from GEA6 and Em clones of wheat in that a sequence coding for 20 amino acid residues is tandemly repeated 4 times. These two genomic clones correspond to two genes named AtEm1 and AtEm6. Sequencing of several cDNA clones showed that both genes are expressed. The transcription start site was determined for both genes by RNase mapping. The site of polyadenylation is variable and there is no obvious consensus sequence for polyadenylation at the 3′ ends of the genes. mRNA corresponding to GEA6 is present only in nearly dry and dry seeds, whereas that corresponding to GEA1 appears in immature seeds and is maximum in dry seeds. No expression of either gene could be detected in leaf, stem, or floral buds. Expression of both genes could be detected in immature seeds when the siliques were incubated with abscisic acid (ABA), demonstrating that both genes are ABA responsive. However, examination of the 5′ upstream region does not reveal any extensive homology, suggesting that regulation of the two genes differs. In situ hybridization with a GEA1 probe demonstrated that the expression of this gene is essentially located in the provascular tissues of the cotyledons and axis of the dry seed as well as in the epiderm and outer layers of the cortex in the embryo axis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292000
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  • 2
    Electronic Resource
    Electronic Resource
    Expression and cellular localization of Atrab28 during Arabidopsis embryogenesis (1999)
    Springer
    Plant molecular biology 40 (1999), S. 355-363 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; embryo-specific gene expression ; Lea proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)+ stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006219315562
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    Paper (German National Licenses)
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