ISSN:
1432-1335
Keywords:
Key wordsp16
;
Radiosensitivity
;
Cell cycle
;
Cell line
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To assess the role played by p16 gene expression in the radiosensitivity of human lung cancers, we transferred exogenous p16 genes into p16-deficient H460 and A549 lung adenocarcinoma cell lines and compared the cell survival curve in vitro after irradiation. The surviving fraction of the p16-transfected A549p16 and H460p16 cells that expressed exogenous p16 mRNA or protein was lower than those of the parental and negative control cells. The rapid exit of the p16-transfected cells from the G2/M phase in the cell cycle, both before and after irradiation, possibly contributes to the increased radiosensitivity of our experimental p16-transfected lung adenocarcinoma cell lines. We conclude that exogenous p16 gene may be another important factor controlling the intrinsic cellular radiosensitivity of cancer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004320050224
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