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  • 1
    Electronic Resource
    Electronic Resource
    Head and stalk structures of soybean vacuolar membranes (1991)
    Springer
    Planta 184 (1991), S. 343-349 
    Details
    ISSN: 1432-2048
    Keywords: Glycine ; Vacuolar H−+-ATP-ase ; Tonoplast (negative staining)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Highly purified tonoplast fractions isolated by preparative free-flow electrophoresis from hypocotyls of etiolated soybean (Glycine max L. (Merr.)) were examined by negative-staining electron microscopy, and many but not all vesicles were found to exhibit head and stalk structures resembling the 9-nm stalked F1 ATPase particles reported previously for Neurospora (Bowman et al., 1989, J. Biol. Chem. 264, 15606–15612). The structures show distinguishing characteristics similar to those for Neurospora. These include a cleft in the particle not exhibited by mitochondrial F1 ATPase and a tendency to disappear from the membrane when treated with nitrate plus Mg−2+-ATP-containing solutions. The position of the stalked ATPase structures, indicates that some of the tonoplast vesicles were oriented cytoplasmic side out whereas others were oriented cytoplasmic side in.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195335
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Identification of tonoplast fractions resolved from plasma membrane by free-flow electrophoresis using filipin-labeling and antibody to the tonoplast ATPase (1990)
    Springer
    Protoplasma 156 (1990), S. 57-66 
    Details
    ISSN: 1615-6102
    Keywords: Free-flow electrophoresis ; Filipin ; Tonoplast ; Plasma Membrane ; Tonoplast ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Preparative free-flow electrophoresis has been employed in combination with density gradient centrifugation to prepare fractions enriched in either tonoplast or plasma membrane from dark grown seedlings of cress (roots), zucchini (hypocotyls), soybean (hypocotyls) and maize (coleoptiles). A polyclonal antibody to the 72,000 Mr subunit to the maize tonoplast ATPase was used to identify the tonoplast fractions from the free-flow electrophoresic separations and to show the absence of tonoplast contamination in plasma membranes derived from the same homogenates. These findings confirm the identity of the tonoplast fraction based on the presence of the proton translocating ATPase determined previously from sucrose gradient fractionation and inhibitor studies to be a tonoplast marker. Using staining with phosphotungstic acid at low pH, the plasma membrane fractions obtained after free-flow electrophoresis were shown to be 〉 90% plasma membrane-derived with little or no cross-contamination of plasma membrane vesicles in the tonoplast-containing fractions. Finally, the composition of the fractions was correlated with the characteristic morphologic appearance after filipin treatment and freeze-fracture. By means of morphometric analyses using this criterion, both the identity and the purity of the tonoplast and the plasma membrane fractions received further confirmation. Essentially homogeneous fractions were obtained by subjecting fractions already enriched by a centrifugation method to the final separation by free-flow electrophoresis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01666506
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Unknown
    Resource productivity in 7 steps : how to develop eco-innovative products and services and improve their material footprint (2009)
    Wuppertal : Wuppertal Institute for Climate, Environment and Energy | Wuppertal : Wuppertal Institut für Klima, Umwelt, Energie
    Details
    Publication Date: 2022-02-18
    Description: This compendium "Resource Productivity in 7 Steps" is intended to give practical advice to designers, engineers, distributors, banks, lawmakers and others how to increase the resource productivity of goods and services (dematerialisation). The eco-innovative (re-)design of products begins with the definition/description of the benefit or service, which a product provides to its user. The use of MIPS (Material Input Per unit Service) helps to develop solutions that can provide this benefit with the least possible quantity of natural resources, from. It measures the material and energy input of a product throughout its life-cycle, "from cradle to cradle" (production of raw materials, manufacturing, transportation, use, disposal). Thus, material and energy consumption can be minimised while satisfying the demand and decoupling of the economic activities from resource use. The brochure describes in seven steps how to gain more resource productivity. It provides several worksheets for the innovation process and material intensity factors for the calculation of the material footprint. A translation into traditional chinese is also available.
    Keywords: ddc:600
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: report , doc-type:report
    Format: application/pdf
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    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    MyEcoCost - forming the nucleus of a novel environmental accounting system : vision, prototype and way forward (2015)
    Wuppertal : Wuppertal Institute for Climate, Environment and Energy
    Details
    Publication Date: 2022-11-10
    Description: The innovative software system "myEcoCost" enables to gather and communicate resource and environmental data for products and services in global value chains. The system has been developed in the consortium of the European research project myEcoCost and forms a basis of a new, highly automated environmental accounting system für companies and consumers. The prototype of the system, linked to financial accounting of companies, was developed and tested in close collaboration with large and small companies. This brochure gives a brief introduction to the vision linked to myEcoCost: a network formed by collaborative environmental accounting nodes collecting environmental data at each step in a product's value chains. It shows why better life cycle data are needed and how myEcoCost addresses and solves this problem. Furthermore, it presents options for a future upscaling of highly automated environmenal accounting for prodcuts and services.
    Keywords: ddc:600
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: report , doc-type:report
    Format: application/pdf
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    PAPER (OA)
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