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  • 1
    Online Resource
    Online Resource
    Scientific Societies ; 2021
    In:  Plant Health Progress Vol. 22, No. 2 ( 2021-01-01), p. 176-181
    In: Plant Health Progress, Scientific Societies, Vol. 22, No. 2 ( 2021-01-01), p. 176-181
    Abstract: Fusarium crown rot of winter wheat is an economically important disease in most regions where winter wheat is grown. Fusarium crown rot is caused by Fusarium culmorum and F. pseudograminearum. This diagnostic guide details information to aid in field, molecular, and morphological diagnosis of Fusarium crown rot.
    Type of Medium: Online Resource
    ISSN: 1535-1025
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2021
    detail.hit.zdb_id: 2115009-6
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  • 2
    Online Resource
    Online Resource
    Scientific Societies ; 2023
    In:  Phytobiomes Journal Vol. 7, No. 1 ( 2023-06), p. 100-112
    In: Phytobiomes Journal, Scientific Societies, Vol. 7, No. 1 ( 2023-06), p. 100-112
    Abstract: Plant is one of the primary drivers of microbial communities in the rhizosphere. The consistent presence of the same plant species over time such as monocropping in agriculture can drive significant changes in plant-associated microbiomes. Most of the studies with monocropping have focused on bacteria, which are involved in the natural suppression of a number of soilborne diseases, including Rhizoctonia root rot and take-all. However, few studies have examined how monocropping and root rot pathogens jointly affect the structure of fungal communities in the rhizosphere. In this greenhouse study, rhizosphere fungal communities from successive wheat plantings infected with the fungal pathogen Rhizoctonia solani AG8 were characterized using MiSeq sequencing targeting the internal transcribed spacer 1 region of the ribosomal RNA gene. Sequence analyses revealed that distinct fungal groups clustered by planting cycles with or without strain AG8 inoculation but infection with strain AG8 enhanced the separation of fungal communities. Clusters of fungal communities were also observed in strain-AG8-infected and noninfected rhizospheres, whereas there was no difference in fungal communities between the rhizospheres with the least root disease and those with the worst root disease. Planting cycles significantly reduced fungal α diversity. The most abundant fungal genus was Mortierella which increased in relative abundance with planting cycles in strain-AG8-infected samples. In contrast, fungal genera that included Pseudogymnoascus, Gibberella, Fusarium, Fusicolla, Exophiala, and Waitea were reduced in relative abundance with successive plantings and strain AG8 infection. Together, this study revealed how fungal communities change with successive wheat growth under the pressure of a soilborne fungal pathogen.
    Type of Medium: Online Resource
    ISSN: 2471-2906
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2023
    detail.hit.zdb_id: 2897163-2
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  • 3
    Online Resource
    Online Resource
    Scientific Societies ; 2020
    In:  Plant Disease Vol. 104, No. 10 ( 2020-10), p. 2649-2657
    In: Plant Disease, Scientific Societies, Vol. 104, No. 10 ( 2020-10), p. 2649-2657
    Abstract: No-till or direct seeding can be described as seeding directly into the crop stubble from the previous season without use of tillage. A reduction in tillage can result in many benefits, including increased soil organic matter, increased water holding capacity, and reduced fuel costs. However, the effect of no-till and reduced tillage on crop root disease profiles is poorly understood. To study the effect of tillage on disease dynamics, soil samples were collected from commercial wheat fields representing a wide range of tillage strategies in fall 2016 and fall 2017. Because precipitation might affect soilborne diseases, wheat fields located across a diverse gradient of precipitation zones of the dryland Pacific Northwest were selected. Fusarium spp., Pythium spp., and Rhizoctonia spp. were quantified from soil samples using soil dilution plating and quantitative PCR (qPCR) assays. Results of dilution plating showed that the colony counts of Fusarium, Pythium, and Rhizoctonia at the genus level were negatively associated with tillage. However, the same patterns were not observed when specific causal agents of Fusarium, Pythium, and Rhizoctonia that are known to be pathogenic on wheat were quantified with qPCR. Furthermore, precipitation affected the population density of some fungal pathogens (F. culmorum, P. ultimum, and R. solani AG 8). Within the scope of inference of this study, results of this study indicate that the benefits of adopting reduced tillage likely outweigh potential risk for increased root disease.
    Type of Medium: Online Resource
    ISSN: 0191-2917 , 1943-7692
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2020
    detail.hit.zdb_id: 2042679-3
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  • 4
    In: Molecular Plant-Microbe Interactions®, Scientific Societies, Vol. 31, No. 11 ( 2018-11), p. 1117-1120
    Abstract: Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.
    Type of Medium: Online Resource
    ISSN: 0894-0282 , 1943-7706
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2018
    detail.hit.zdb_id: 2037108-1
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Scientific Societies ; 2024
    In:  Plant Disease Vol. 108, No. 6 ( 2024-06-01), p. 1565-1576
    In: Plant Disease, Scientific Societies, Vol. 108, No. 6 ( 2024-06-01), p. 1565-1576
    Abstract: Soybean (Glycine max [L.] Merr.) is an important oilseed crop with a high economic value. However, three damaging soybean diseases, soybean cyst nematode (SCN; Heterodera glycines Ichinohe), Sclerotinia stem rot caused by the fungus Sclerotinia sclerotiorum (Lid.) de Bary, and soybean root rot caused by Fusarium spp., are major constraints to soybean production in the Great Plains. Current disease management options, including resistant or tolerant varieties, fungicides, nematicides, and agricultural practices (crop rotation and tillage), have limited efficacy for these pathogens or have adverse effects on the ecosystem. Microbes with antagonistic activity are a promising option to control soybean diseases with the advantage of being environmentally friendly and sustainable. In this study, 61 bacterial strains isolated from wheat rhizospheres were used to examine their antagonistic abilities against three soybean pathogens. Six bacterial strains significantly inhibited the growth of Fusarium graminearum in the dual-culture assay. These bacterial strains were identified as Chryseobacterium ginsengisoli, C. indologenes, Pseudomonas poae, two Pseudomonas spp., and Delftia acidovorans by 16S rRNA gene sequencing. Moreover, C. ginsengisoli, C. indologenes, and P. poae significantly increased the mortality of SCN second-stage juveniles (J2), and two Pseudomonas spp. inhibited the growth of S. sclerotiorum in vitro. Further growth chamber tests found that C. ginsengisoli and C. indologenes reduced soybean Fusarium root rot disease. C. ginsengisoli and P. poae dramatically decreased SCN egg number on SCN-susceptible soybean ‘Williams 82’. Two Pseudomonas spp. protected soybean plants from leaf damage and collapse after being infected by S. sclerotiorum. These bacteria exhibit versatile antagonistic potential. This work lays the foundation for further research on the field control of soybean pathogens.
    Type of Medium: Online Resource
    ISSN: 0191-2917 , 1943-7692
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2024
    detail.hit.zdb_id: 2042679-3
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  • 6
    Online Resource
    Online Resource
    Scientific Societies ; 2011
    In:  Molecular Plant-Microbe Interactions® Vol. 24, No. 5 ( 2011-05), p. 554-561
    In: Molecular Plant-Microbe Interactions®, Scientific Societies, Vol. 24, No. 5 ( 2011-05), p. 554-561
    Abstract: Rust fungi cause devastating diseases of wheat and other cereal species globally. Genetic resistance is the preferred method to control rusts but the effectiveness of race-specific resistance is typically transient due to the genetic plasticity of rust populations. The advent of RNA interference (RNAi) technology has shown promise for the engineering of resistance to some biotrophic pathogens in plants by altering the expression of essential pathogens' genes. Gene fragments from the rust fungi Puccinia striiformis f. sp. tritici or P. graminis f. sp. tritici were delivered to plant cells through the Barley stripe mosaic virus system, and some reduced the expression of the corresponding genes in the rust fungus. The ability to detect suppression was associated with the expression patterns of the fungal genes because reduction was only detected in transcripts with relatively high levels of expression in fungal haustoria. The results indicate that an in planta RNAi approach can be used in functional genomics research for rust fungi and that it could potentially be used to engineer durable resistance.
    Type of Medium: Online Resource
    ISSN: 0894-0282 , 1943-7706
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2011
    detail.hit.zdb_id: 2037108-1
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Scientific Societies ; 2019
    In:  Phytopathology® Vol. 109, No. 9 ( 2019-09), p. 1509-1512
    In: Phytopathology®, Scientific Societies, Vol. 109, No. 9 ( 2019-09), p. 1509-1512
    Abstract: Stripe rust caused by Puccinia striiformis is a disastrous disease of cereal crops and various grasses. To date, 14 stripe rust genomes are publicly available, including 13 P. striiformis f. sp. tritici and 1 P. striiformis f. sp. hordei. In this study, one isolate (11-281) of P. striiformis collected from wheatgrass (Agropyron cristatum), which is avirulent to most of standard differential genotypes of wheat and barley, was sequenced, assembled, and annotated. The sequences were assembled to a draft genome of 84.75 Mb, which is comparable with previously sequenced P. striiformis f. sp. tritici and P. striiformis f. sp. hordei isolates. The draft genome comprised 381 scaffolds and contained 1,829 predicted secreted proteins. The high-quality draft genome of the isolate is a valuable resource in shedding light on the evolution and pathogenicity of P. striiformis.
    Type of Medium: Online Resource
    ISSN: 0031-949X , 1943-7684
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2019
    detail.hit.zdb_id: 2037027-1
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Scientific Societies ; 2017
    In:  Phytopathology® Vol. 107, No. 1 ( 2017-01), p. 75-83
    In: Phytopathology®, Scientific Societies, Vol. 107, No. 1 ( 2017-01), p. 75-83
    Abstract: Fungi that cause cereal rust diseases (genus Puccinia) are important pathogens of wheat globally. Upon infection, the fungus secretes a number of effector proteins. Although a large repository of putative effectors has been predicted using bioinformatic pipelines, the lack of available high-throughput effector screening systems has limited functional studies on these proteins. In this study, we mined the available transcriptomes of Puccinia graminis and P. striiformis to look for potential effectors that suppress host hypersensitive response (HR). Twenty small ( 〈 300 amino acids), secreted proteins, with no predicted functions were selected for the HR suppression assay using Nicotiana benthamiana, in which each of the proteins were transiently expressed and evaluated for their ability to suppress HR caused by four cytotoxic effector‐R gene combinations (Cp/Rx, ATR13/RPP13, Rpt2/RPS‐2, and GPA/RBP‐1) and one mutated R gene—Pto(Y207D). Nine out of twenty proteins, designated Shr1 to Shr9 (suppressors of hypersensitive response), were found to suppress HR in N. benthamiana. These effectors varied in the effector-R gene defenses they suppressed, indicating these pathogens can interfere with a variety of host defense pathways. In addition to HR suppression, effector Shr7 also suppressed PAMP-triggered immune response triggered by flg22. Finally, delivery of Shr7 through Pseudomonas fluorescens EtHAn suppressed nonspecific HR induced by Pseudomonas syringae DC3000 in wheat, confirming its activity in a homologous system. Overall, this study provides the first evidence for the presence of effectors in Puccinia species suppressing multiple plant defense responses.
    Type of Medium: Online Resource
    ISSN: 0031-949X , 1943-7684
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2017
    detail.hit.zdb_id: 2037027-1
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Scientific Societies ; 2007
    In:  Molecular Plant-Microbe Interactions® Vol. 20, No. 3 ( 2007-03), p. 223-234
    In: Molecular Plant-Microbe Interactions®, Scientific Societies, Vol. 20, No. 3 ( 2007-03), p. 223-234
    Abstract: The Pseudomonas syringae type III secretion system (T3SS) is induced during interaction with the plant or culture in minimal medium (MM). How the bacterium senses these environments to activate the T3SS is poorly understood. Here, we report the identification of a novel two-component system (TCS), RhpRS, that regulates the induction of P. syringae T3SS genes. The rhpR and rhpS genes are organized in an operon with rhpR encoding a putative TCS response regulator and rhpS encoding a putative biphasic sensor kinase. Transposon insertion in rhpS severely reduced the induction of P. syringae T3SS genes in the plant as well as in MM and significantly compromised the pathogenicity on host plants and hypersensitive response-inducing activity on nonhost plants. However, deletion of the rhpRS locus allowed the induction of T3SS genes to the same level as in the wild-type strain and the recovery of pathogenicity upon infiltration into plants. Overexpression of RhpR in the ΔrhpRS deletion strain abolished the induction of T3SS genes. However, overexpression of RhpR in the wild-type strain or overexpression of RhpR(D70A), a mutant of the predicted phosphorylation site of RhpR, in the ΔrhpRS deletion strain only slightly reduced the induction of T3SS genes. Based on these results, we propose that the phosphorylated RhpR represses the induction of T3SS genes and that RhpS reverses phosphorylation of RhpR under the T3SS-inducing conditions. Epistasis analysis indicated that rhpS and rhpR act upstream of hrpR to regulate T3SS genes.
    Type of Medium: Online Resource
    ISSN: 0894-0282 , 1943-7706
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2007
    detail.hit.zdb_id: 2037108-1
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Scientific Societies ; 2014
    In:  Molecular Plant-Microbe Interactions® Vol. 27, No. 3 ( 2014-03), p. 227-235
    In: Molecular Plant-Microbe Interactions®, Scientific Societies, Vol. 27, No. 3 ( 2014-03), p. 227-235
    Abstract: The plant hormone indole-3-acetic acid (IAA) is best known as a regulator of plant growth and development but its production can also affect plant–microbe interactions. Microorganisms, including numerous plant-associated bacteria and several fungi, are also capable of producing IAA. The stem rust fungus Puccinia graminis f. sp. tritici induced wheat plants to accumulate auxin in infected leaf tissue. A gene (Pgt-IaaM) encoding a putative tryptophan 2-monooxygenase, which makes the auxin precursor indole-3-acetamide (IAM), was identified in the P. graminis f. sp. tritici genome and found to be expressed in haustoria cells in infected plant tissue. Transient silencing of the gene in infected wheat plants indicated that it was required for full pathogenicity. Expression of Pgt-IaaM in Arabidopsis caused a typical auxin expression phenotype and promoted susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000.
    Type of Medium: Online Resource
    ISSN: 0894-0282 , 1943-7706
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2014
    detail.hit.zdb_id: 2037108-1
    SSG: 12
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