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  • SAGE Publications  (211)
  • 1
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    Expression and methylation status of LAMA2 are associated with the invasiveness of nonfunctioning PitNET
    SAGE Publications ; 2019
    In:  Therapeutic Advances in Endocrinology and Metabolism Vol. 10 ( 2019-01), p. 204201881882129-
    Details
    In: Therapeutic Advances in Endocrinology and Metabolism, SAGE Publications, Vol. 10 ( 2019-01), p. 204201881882129-
    Abstract: The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). In the current study, we investigated the relationship between LAMA2 promoter methylation status and the invasiveness of clinically nonfunctioning pituitary adenomas (PitNETs). Specimens from patients with nonfunctioning PitNET were classified into three groups according to preoperative computed tomography (CT)/magnetic resonance imaging findings: a normal group ( n = 6), non-invasive group ( n = 11) and invasive group ( n = 6). LAMA2 expression was assessed using quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting, and the methylation status of the LAMA2 promoter region was observed using sodium bisulfite sequencing. Furthermore, 5-aza-2-deoxycytidine was used to explore the relationship between decreased LAMA expression and methylation in PitNET cells. According to the RT-qPCR and western blotting results, LAMA2 expression was downregulated in invasive PitNET, while the methylation of the LAMA2 promoter was increased. Methylation of the LAMA2 promoter decreased the expression of LAMA2. Thus, changes in LAMA2 expression due to promoter methylation were inversely correlated with the invasiveness of PitNET and the protein functions as a tumor suppressor. In addition, overexpression and demethylation of LAMA2 suppressed the invasion of PitNET cells, partially by exerting effects on the PTEN-PI3K/AKT signaling pathway and matrix metalloproteinase-9 (MMP-9). Furthermore, a xenograft model was also generated, and LAMA2 overexpression significantly suppressed tumor growth in vivo. Thus, LAMA2 expression and methylation patterns might be used as biomarkers to predict the prognosis of patients with PitNET.
    Type of Medium: Online Resource
    ISSN: 2042-0188 , 2042-0196
    URL: Article
    DOI: 10.1177/2042018818821296
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2019
    detail.hit.zdb_id: 2554822-0
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  • 2
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    Different methods of detaching adherent cells significantly affect the detection of TRAIL receptors
    SAGE Publications ; 2012
    In:  Tumori Journal Vol. 98, No. 6 ( 2012-11), p. 800-803
    Details
    In: Tumori Journal, SAGE Publications, Vol. 98, No. 6 ( 2012-11), p. 800-803
    Abstract: As a powerful technique allowing analysis of large numbers of cells, fluorescence-activated cell sorting (FACS) is used more and more widely. For FACS analysis, adherent cells are usually detached by trypsinization, followed by centrifugation and resuspension. However, trypsinization can cut off some receptors from the cell surface like fine scissors, which will affect the accuracy of FACS results. Though non-enzymatic methods such as citric saline buffer have been used to determine cell surface receptors, how much of the receptors is cut off by trypsinization has been rarely studied. This work aimed to investigate whether different methods of detaching adherent cells could affect the detection of cell surface receptors. Methods Human hepatocellular carcinoma cell lines (HepG2, Huh7 and Hep3B) were detached enzymatically with trypsin-EDTA solution or non-enzymatically with citric saline buffer, and then the receptors of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were detected by FACS analysis. Cell viability, cell cycle and apoptosis (sub-G1 fraction detected by FACS) of the trypsin-EDTA group and citric saline buffer group were also studied. Results Different methods of detaching adherent cells could significantly affect the detection of TRAIL receptors. Compared to the conventional trypsin-EDTA group, the non-enzymatic group showed a 3.42-fold increase in the mean fluorescence intensity index of DcR HepG2 and a 1.25-fold increase in DR Huh 7 (P 〈 0.05). However, the viability, cell cycle and apoptosis of these cells were not affected. Conclusions Citric saline buffer might be recommended as the first choice to detach adherent cells for FACS analysis of cell surface receptors.
    Type of Medium: Online Resource
    ISSN: 0300-8916 , 2038-2529
    URL: Article
    DOI: 10.1177/030089161209800619
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2012
    detail.hit.zdb_id: 280962-X
    detail.hit.zdb_id: 2267832-3
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  • 3
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    Coexistent pulmonary cryptococcal infection and pulmonary sarcoidosis: a case report and literature review
    SAGE Publications ; 2020
    In:  Journal of International Medical Research Vol. 48, No. 2 ( 2020-02), p. 030006052090387-
    Details
    In: Journal of International Medical Research, SAGE Publications, Vol. 48, No. 2 ( 2020-02), p. 030006052090387-
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    URL: Article
    DOI: 10.1177/0300060520903870
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2082422-1
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  • 4
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    Characterization and Inference of Gene Gain/Loss along Burkholderia Evolutionary History
    SAGE Publications ; 2011
    In:  Evolutionary Bioinformatics Vol. 7 ( 2011-01), p. EBO.S7510-
    Details
    In: Evolutionary Bioinformatics, SAGE Publications, Vol. 7 ( 2011-01), p. EBO.S7510-
    Abstract: A comparative analysis of 60 complete Burkholderia genomes was conducted to obtain insight in the evolutionary history behind the diversity and pathogenicity at species level. A concatenated multiprotein phyletic pattern and a dataset with Burkholderia clusters of orthologous genes (BuCOGs) were constructed. The extent of horizontal gene transfer (HGT) was assessed using a Markov based probabilistic method. A reconstruction of the gene gains and losses history shows that more than half of the Burkholderia genes families are inferred to have experienced HGT at least once during their evolution. Further analysis revealed that the number of gene gain and loss was correlated with the branch length. Genomic islands (GEIs) analysis based on evolutionary history reconstruction not only revealed that most genes in ancient GEIs were gained but also suggested that the fraction of the genome located in GEIs in the small chromosomes is higher than in the large chromosomes in Burkholderia. The mapping of coexpressed genes onto biological pathway schemes revealed that pathogenicity of Burkholderia strains is probably mainly determined by the gained genes in its ancestor. Taken together, our results strongly support that gene gain and loss especially in ancient evolutionary history play an important role in strain divergence, pathogenicity determinants of Burkholderia and GEIs formation.
    Type of Medium: Online Resource
    ISSN: 1176-9343 , 1176-9343
    URL: Article
    DOI: 10.4137/EBO.S7510
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
    detail.hit.zdb_id: 2227610-5
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  • 5
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    Infiltration of nanocarbon suspension into the tracheal cavity during surgical treatment of papillary thyroid carcinoma: a case report
    SAGE Publications ; 2020
    In:  Journal of International Medical Research Vol. 48, No. 4 ( 2020-04), p. 030006052091925-
    Details
    In: Journal of International Medical Research, SAGE Publications, Vol. 48, No. 4 ( 2020-04), p. 030006052091925-
    Abstract: Cervical lymph node metastasis in patients with differentiated thyroid cancer affects postoperative recurrence and survival. Hypoparathyroidism is caused by parathyroid gland injury during thyroidectomy. Carbon nanoparticles can trace stained lymph nodes, aiding in thorough dissection of lymph nodes in the operation area. To reduce postoperative occurrence of hypoparathyroidism, the parathyroid glands and their functions (identified by negative imaging induced by carbon nanoparticles) are retained in situ. However, the safety and adverse effects of nanocarbon suspension in thyroid surgery have rarely been evaluated. In this report, we describe a patient with thyroid cancer who had carbon secretions in the trachea caused by nanocarbon suspension when tracheal intubation was performed under general anesthesia, and the inflatable balloon surface of the tracheal tube was covered with these secretions. The patient recovered without fever, cough, phlegm production, chest pain, hoarseness, or hypocalcemia-induced convulsions. No consensus has yet been reached on the most appropriate injection site, depth, dose, or waiting time for nanocarbons in thyroid cancer surgery. We believe that nanocarbon suspension is safe for use in thyroid cancer surgery, but the most appropriate injection depth should be based on the thickness of the thyroid gland tissue to avoid deep injection into the trachea.
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    URL: Article
    DOI: 10.1177/0300060520919251
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2082422-1
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  • 6
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    Blockade of LINC01605-enriched exosome generation in M2 macrophages impairs M2 macrophage-induced proliferation, migration, and invasion of human dermal fibroblasts
    SAGE Publications ; 2021
    In:  International Journal of Immunopathology and Pharmacology Vol. 35 ( 2021-01), p. 205873842110167-
    Details
    In: International Journal of Immunopathology and Pharmacology, SAGE Publications, Vol. 35 ( 2021-01), p. 205873842110167-
    Abstract: Activated M2 macrophages are involved in hypertrophic scar (HS) formation via manipulating the differentiation of fibroblasts to myofibroblasts having the proliferative capacity and biological function. However, the function of exosomes derived from M2 macrophages in HS formation is unclear. Thus, this study aims to investigate the role of exosomes derived by M2 in the formation of HS. To understand the effect of exosomes derived from M2 macrophages on formation of HS, M2 macrophages were co-cultured with human dermal fibroblast (HDF) cells. Cell Counting Kit-8 assay was performed to evaluate HDF proliferation. To evaluate the migration and invasion of HDFs, wound-healing and transwell invasion assays were performed, respectively. To investigate the interaction between LINC01605 and miR-493-3p, a dual-luciferase reporter gene assay was adopted; consequently, an interaction between miR-493-3p and AKT1 was detected. Our results demonstrated that exosomes derived from M2 macrophages promoted the proliferation, migration, and invasion of HDFs. Additionally, we found that long noncoding RNA LINC01605, enriched in exosomes derived from M2 macrophages, promoted fibrosis of HDFs and that GW4869, an inhibitor of exosomes, could revert this effect. Mechanistically, LINC01605 promoted fibrosis of HDFs by directly inhibiting the secretion of miR-493-3p, and miR-493-3p down-regulated the expression of AKT1. Exosomes derived from M2 macrophages promote the proliferation and migration of HDFs by transmitting LINC01605, which may activate the AKT signaling pathway by sponging miR-493-3p. Our results provide a novel approach and basis for further investigation of the function of M2 macrophages in HS formation.
    Type of Medium: Online Resource
    ISSN: 2058-7384 , 2058-7384
    URL: Article
    DOI: 10.1177/20587384211016724
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2505963-4
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  • 7
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    Tracheobronchial foreign body aspiration in pediatric patients: An experience on 1060 cases in 2015
    SAGE Publications ; 2017
    In:  European Journal of Inflammation Vol. 15, No. 3 ( 2017-12), p. 267-271
    Details
    In: European Journal of Inflammation, SAGE Publications, Vol. 15, No. 3 ( 2017-12), p. 267-271
    Abstract: This study aims to investigate the clinical characteristics of tracheobronchial foreign bodies (TFBs) in children and its methods of treatment, providing a theoretical basis for including TFBs into the clinical pathway for children and conducting bronchoscopy performed by senior residents and attending physicians under general anesthesia. The clinical data of 1060 patients diagnosed with TFBs from January 2015 to January 2016 were evaluated. The age, gender, clinical manifestations, foreign body properties, thoracic CT, and three-dimensional reconstruction, preoperative and postoperative complications, hospital stay, surgical and general anesthesia bronchoscopy, and foreign body removal surgery of these patients were analyzed. TFBs frequently occurred in 0- to 3-year-old patients, accounting for 92.5%, and 64.3% of these patients were male. There is no evident difference in foreign bodies detected in the left and right bronchus. Foreign bodies are mainly botanic, accounting for 88.9%, among which peanuts and melon seeds were mostly observed. All pediatric patients received tracheobronchoscopy under general anesthesia, and 97.3% of these surgeries were performed by senior residents and attending physicians. No complication or death occurred after the surgery. TFBs can be treated according to the clinical pathway. The timely and accurate diagnosis of TFBs and its performance under general anesthesia can evidently reduce the mortality rate. Senior residents and attending physicians can be qualified to perform the bronchoscopy after training.
    Type of Medium: Online Resource
    ISSN: 2058-7392 , 2058-7392
    URL: Article
    DOI: 10.1177/1721727X17744994
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2584683-8
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  • 8
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    Evaluation of carotid atherosclerosis and related risk factors using ultrasonic B-Flow technology in elderly patients
    SAGE Publications ; 2020
    In:  Journal of International Medical Research Vol. 48, No. 10 ( 2020-10), p. 030006052096122-
    Details
    In: Journal of International Medical Research, SAGE Publications, Vol. 48, No. 10 ( 2020-10), p. 030006052096122-
    Abstract: This study was performed to identify the risk factors for carotid atherosclerotic plaque formation using B-Flow ultrasound. Methods In total, 120 patients who underwent bilateral carotid ultrasound examination were enrolled in this cross-sectional study. The intima–media thickness was measured, and the risk factors for carotid atheromatous plaque formation were investigated. Results Age, sex, medical history of hypertension, coronary heart disease, and diabetes were risk factors for carotid atheromatous plaque formation. Multivariate logistic regression analysis revealed that the main risk factors for carotid atheromatous plaque formation were male sex, advanced age, a high hemoglobin concentration, a high red cell distribution width, and a high low-density lipoprotein cholesterol concentration. Conclusion The risk factors for carotid atheromatous plaque formation were basically the same as those for stroke. Early ultrasound examination of the carotid artery enables the identification of risk factors associated with stroke.
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    URL: Article
    DOI: 10.1177/0300060520961224
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2082422-1
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  • 9
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    Mast Cell Contributes to Cardiomyocyte Apoptosis after Coronary Microembolization
    SAGE Publications ; 2006
    In:  Journal of Histochemistry & Cytochemistry Vol. 54, No. 5 ( 2006-05), p. 515-523
    Details
    In: Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 54, No. 5 ( 2006-05), p. 515-523
    Abstract: Coronary microembolization (CME) is associated with progressive myocardial dysfunction despite restoration of coronary flow reserve (CFR). The potential pathophysiological role of mast cells (MCs) remains unclear. Therefore, we induced CME in 18 miniswines and determined whether MC accumulation occurs and their effects on local cytokine secretion [interleukin (IL)-6, IL-8, tumor necrosis factor-α (TNF-α)]; cardiomyocyte apoptosis; and collagen formation at day 1 (D1), day 7 (D7), and day 30 (D30) after CME. Four sham-operated animals without CME (controls) and six animals treated with a MC stabilization agent (tranilast) for 30 days after CME were also studied. CFR decreased at D1 but returned to baseline level at D7 and D30. Coronary sinus levels of IL-6, IL-8, and TNF-α increased significantly at D1 and D7 ( p 〈 0.01 vs baseline). Levels of IL-6 and IL-8 at D30 returned to baseline level, but not those of TNF-α. The numbers of total and degranulating MCs, % apoptotic cardiomyocytes, and collagen volume fraction (CVF) over CME myocardium at D1, D7, and D30 were significantly higher than controls ( p 〈 0.01). Treatment with tranilast significantly reduced the serum level of TNF-α, numbers of total and degranulating MCs, % apoptotic cardiomyocytes, and CVF at D30 (all p 〈 0.05). There was a significant positive correlation between the numbers of MCs with % apoptotic cardiomyocytes ( r = 0.77, p 〈 0.001) and CVF ( r = 0.75, p 〈 0.001) over the CME myocardium. Despite restoration of CFR, cardiomyocyte apoptosis persisted after CME and was positively correlated with the number of MCs but was prevented with tranilast treatment. These findings suggest that MCs contribute to cardiomyocyte apoptosis after CME. (J Histochem Cytochem 54:515-523, 2006)
    Type of Medium: Online Resource
    ISSN: 0022-1554 , 1551-5044
    URL: Article
    DOI: 10.1369/jhc.5A6804.2005
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2006
    detail.hit.zdb_id: 1421306-0
    SSG: 12
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  • 10
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    Effect of Helicobacter Pylori on Plasma Metabolic Phenotype in Patients With Gastric Cancer
    SAGE Publications ; 2021
    In:  Cancer Control Vol. 28 ( 2021-01), p. 107327482110418-
    Details
    In: Cancer Control, SAGE Publications, Vol. 28 ( 2021-01), p. 107327482110418-
    Abstract: Although  Helicobacter pylori (Hp) as high risk factor for gastric cancer have been investigated from human trial, present data is inadequate to explain the effect of Hp on the changes of metabolic phenotype of gastric cancer in different stages. Purpose Herein, plasma of human superficial gastritis (Hp negative and positive), early gastric cancer and advanced gastric cancer analyzed by UPLC-HDMS metabolomics can not only reveal metabolic phenotype changes in patients with gastric cancer of different degrees (30 Hp negative, 30 Hp positive, 20 early gastric cancer patients, and 10 advanced gastric cancer patients), but also auxiliarily diagnose gastric cancer. Results Combined with multivariate statistical analysis, the results represented biomarkers different from Hp negative, Hp positive, and the alterations of metabolic phenotype of gastric cancer patients. Forty-three metabolites are involved in amino acid metabolism, and lipid and fatty acid metabolism pathways in the process of cancer occurrence, especially 2 biomarkers glycerophosphocholine and neopterin, were screened in this study. Neopterin was consistently increased with gastric cancer progression and glycerophosphocholine tended to consistently decrease from Hp negative to advanced gastric cancer. Conclusion This method could be used for the development of rapid targeted methods for biomarker identification and a potential diagnosis of gastric cancer.
    Type of Medium: Online Resource
    ISSN: 1073-2748 , 1073-2748
    URL: Article
    DOI: 10.1177/10732748211041881
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2004182-2
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