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  • 1
    Online Resource
    Online Resource
    Restoration of Noradrenergic Function in Parkinson’s Disease Model Mice
    SAGE Publications ; 2021
    In:  ASN Neuro Vol. 13 ( 2021-01), p. 175909142110097-
    Details
    In: ASN Neuro, SAGE Publications, Vol. 13 ( 2021-01), p. 175909142110097-
    Abstract: Dysfunction of the central noradrenergic and dopaminergic systems is the primary neurobiological characteristic of Parkinson’s disease (PD). Importantly, neuronal loss in the locus coeruleus (LC) that occurs in early stages of PD may accelerate progressive loss of dopaminergic neurons. Therefore, restoring the activity and function of the deficient noradrenergic system may be an important therapeutic strategy for early PD. In the present study, the lentiviral constructions of transcription factors Phox2a/2b, Hand2 and Gata3, either alone or in combination, were microinjected into the LC region of the PD model VMAT2 Lo mice at 12 and 18 month age. Biochemical analysis showed that microinjection of lentiviral expression cassettes into the LC significantly increased mRNA levels of Phox2a, and Phox2b, which were accompanied by parallel increases of mRNA and proteins of dopamine β-hydroxylase (DBH) and tyrosine hydroxylase (TH) in the LC. Furthermore, there was considerable enhancement of DBH protein levels in the frontal cortex and hippocampus, as well as enhanced TH protein levels in the striatum and substantia nigra. Moreover, these manipulations profoundly increased norepinephrine and dopamine concentrations in the striatum, which was followed by a remarkable improvement of the spatial memory and locomotor behavior. These results reveal that over-expression of these transcription factors in the LC improves noradrenergic and dopaminergic activities and functions in this rodent model of PD. It provides the necessary groundwork for the development of gene therapies of PD, and expands our understanding of the link between the LC-norepinephrine and dopamine systems during the progression of PD.
    Type of Medium: Online Resource
    ISSN: 1759-0914 , 1759-0914
    URL: Article
    DOI: 10.1177/17590914211009730
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2485467-0
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  • 2
    Online Resource
    Online Resource
    α-Synuclein in Parkinson’s Disease: Does a Prion-Like Mechanism of Propagation from Periphery to the Brain Play a Role?
    SAGE Publications ; 2021
    In:  The Neuroscientist Vol. 27, No. 4 ( 2021-08), p. 367-387
    Details
    In: The Neuroscientist, SAGE Publications, Vol. 27, No. 4 ( 2021-08), p. 367-387
    Abstract: Parkinson’s disease (PD) is one of the most common neurodegenerative diseases, defined as motor and non-motor symptoms associated with the loss of dopaminergic neurons and a decreased release of dopamine (DA). Currently, PD patients are believed to have a neuropathological basis denoted by the presence of Lewy bodies (LBs) or Lewy neurites (LNs), which mostly comprise α-synuclein (α-syn) inclusions. Remarkably, there is a growing body of evidence indicating that the inclusions undergo template-directed aggregation and propagation via template-directed among the brain and peripheral organs, mainly in a prion-like manner. Interestingly, some studies reported that an integral loop was reminiscent of the mechanism of Parkinson’s disease, denoting that α-syn as prionoid was transmitted from the periphery to the brain via specific pathways. Also the systematic life cycle of α-syn in the cellular level is illustrated. In this review, we critically assess landmark evidence in the field of Parkinson’s disease with a focus on the genesis and prion-like propagation of the α-syn pathology. The anatomical and cell-to-cell evidences are discussed to depict the theory behind the propagation and transferred pathways. Furthermore, we highlight effective therapeutic perspectives and clinical trials targeting prion-like mechanisms. Major controversies surrounding this topic are also discussed.
    Type of Medium: Online Resource
    ISSN: 1073-8584 , 1089-4098
    URL: Article
    DOI: 10.1177/1073858420943180
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2029471-2
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  • 3
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    Online Resource
    Detection and Identification of Potential Biomarkers of Non-small Cell Lung Cancer
    SAGE Publications ; 2009
    In:  Technology in Cancer Research & Treatment Vol. 8, No. 6 ( 2009-12), p. 455-465
    Details
    In: Technology in Cancer Research & Treatment, SAGE Publications, Vol. 8, No. 6 ( 2009-12), p. 455-465
    Abstract: The aim of this study was to discover and identify potential protein biomarkers specific for non-small cell lung cancer (NSCLC). Two hundred and thirty five (235) Serum samples with 112 NSCLC and 123 controls were randomly divided into a training set and a blind testing set. Serum proteomic profiles were analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS). Candidate protein biomarkers were purified by high Performance Liquid Chromatography (HPLC) and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) and validated using ProteinChip immunoassays. A total of 3 peaks ( m/z with 6628, 9191 and 11412 Da) were screened out by SVM to construct the classification model with the high discriminatory power in the training set. The sensitivity and specificity of the model were 96.56% and 94.79% respectively in the blind testing set. The candidate biomarker with m/z of 6628 Da was found down-regulated in NSCLC patients, and identified as apolipoprotein C-I. Another two candidate biomarkers (9191 and 11412 Da) were found up-regulated in serum of NSCLC patients and identified as haptoglobin alpha-1 chain and S100A4, respectively. We have identified a set of biomarkers that could discriminate NSCLC from non-cancer controls. An efficient strategy, including SELDI-TOF-MS analysis, HPLC purification, MALDI-TOF-MS trace and LC-MS/MS identification, has been proved successfully.
    Type of Medium: Online Resource
    ISSN: 1533-0346 , 1533-0338
    URL: Article
    DOI: 10.1177/153303460900800607
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2009
    detail.hit.zdb_id: 2146365-7
    detail.hit.zdb_id: 2220436-2
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  • 4
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    Advances in research of intracranial vascular stent
    SAGE Publications ; 2017
    In:  Translational Neuroscience and Clinics Vol. 3, No. 3 ( 2017), p. 176-184
    Details
    In: Translational Neuroscience and Clinics, SAGE Publications, Vol. 3, No. 3 ( 2017), p. 176-184
    Type of Medium: Online Resource
    ISSN: 2096-0441
    URL: Article
    DOI: 10.18679/CN11-6030/R.2017.026
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
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  • 5
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    The Expression of Egfl7 in Human Normal Tissues and Epithelial Tumors
    SAGE Publications ; 2013
    In:  The International Journal of Biological Markers Vol. 28, No. 1 ( 2013-01), p. 71-83
    Details
    In: The International Journal of Biological Markers, SAGE Publications, Vol. 28, No. 1 ( 2013-01), p. 71-83
    Abstract: To investigate the expression of Egfl7 in normal adult human tissues and human epithelial tumors. Methods RT-PCR and Western blot were employed to detect Egfl7 expression in normal adult human tissues and 10 human epithelial tumors including hepatocellular carcinoma (HCC), lung cancer, breast cancer, prostate cancer, colorectal cancer, gastric cancer, esophageal cancer, malignant glioma, ovarian cancer and renal cancer. Immunohistochemistry and cytoimmunofluorescence were subsequently used to determine the localization of Egfl7 in human epithelial tumor tissues and cell lines. ELISA was also carried out to examine the serum Egfl7 levels in cancer patients. In addition, correlations between Egfl7 expression and clinicopathological features as well as prognosis of HCC and breast cancer were also analyzed on the basis of immunohistochemistry results. Results Egfl7 was differentially expressed in 19 adult human normal tissues and was overexpressed in all 10 human epithelial tumor tissues. The serum Egfl7 level was also significantly elevated in cancer patients. The increased Egfl7 expression in HCC correlated with vein invasion, absence of capsule formation, multiple tumor nodes and poor prognosis. Similarly, upregulation of Egfl7 in breast cancer correlated strongly with TNM stage, lymphatic metastasis, estrogen receptor positivity, Her2 positivity and poor prognosis. Conclusions Egfl7 is significantly upregulated in human epithelial tumor tissues, suggesting Egfl7 to be a potential biomarker for human epithelial tumors, especially HCC and breast cancer.
    Type of Medium: Online Resource
    ISSN: 1724-6008 , 1724-6008
    URL: Article
    DOI: 10.5301/JBM.2013.10568
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 1475778-3
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  • 6
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    Effectiveness of a Large-Scale Health and Nutritional Education Program on Anemia in Children Younger Than 5 Years in Shifang, a Heavily Damaged Area of Wenchuan Earthquake
    SAGE Publications ; 2015
    In:  Asia Pacific Journal of Public Health Vol. 27, No. 2 ( 2015-03), p. NP2167-NP2176
    Details
    In: Asia Pacific Journal of Public Health, SAGE Publications, Vol. 27, No. 2 ( 2015-03), p. NP2167-NP2176
    Abstract: This study aimed to explore an ideal way to prevent anemia among children younger than 5 years after disasters especially when health care facilities are not enough. A preliminary survey was carried out involving 13 065 children younger than 5 years. Pretested questionnaires were used for data collection and hemoglobin levels were measured. After 12-month intervention, the impact survey involving 2769 children was conducted. Results showed that there were some improvements both in feeding knowledge and practice related to anemia. The total prevalence of anemia decreased from 14.3% to 7.8% ( P 〈 .001), and the severity of anemia also declined. The hemoglobin concentration increased significantly from 118.8 ± 10.5 to 122.0 ± 9.9 g/L ( P 〈 .001). Thus, health and nutritional education could be an ideal way to combat anemia after disasters especially in less developed areas with multiparty cooperation. The methods and experiences of this study may be well worth learning and implementing.
    Type of Medium: Online Resource
    ISSN: 1010-5395 , 1941-2479
    URL: Article
    DOI: 10.1177/1010539513481495
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2394975-2
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  • 7
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    Antihuman T lymphocyte porcine immunoglobulin combined with cyclosporine as first-line immunosuppressive therapy for severe aplastic anemia in China: a large single-center, 10-year retrospective study
    SAGE Publications ; 2023
    In:  Therapeutic Advances in Hematology Vol. 14 ( 2023-01), p. 204062072211460-
    Details
    In: Therapeutic Advances in Hematology, SAGE Publications, Vol. 14 ( 2023-01), p. 204062072211460-
    Abstract: Antihuman T lymphocyte porcine immunoglobulin (p-ATG) has been the most common ATG preparation in immunosuppressive therapy (IST) in Chinese patients with severe aplastic anemia (SAA) since 2009. Objectives: This study aimed to evaluate the early hematologic response and long-term outcomes of a large cohort of patients with SAA who received p-ATG plus cyclosporine (CsA) as first-line therapy from 2010 to 2019. Design: This is a single-center retrospective study of medical records. Methods: We analyzed the data of 1023 consecutive patients with acquired aplastic anemia (AA) who underwent p-ATG combined with CsA as a first-line IST treatment from 2010 to 2019 at our department. Results: The median age of the patients was 24 (4–75) years, and the median follow-up time was 57.2 months (3 days–137.5 months). There was an early mortality rate of 2.8% with a median death time of 0.9 months (3 days–2.9 months). The overall response rates were 40.6% and 56.1% at 3 and 6 months, respectively. The 5-year cumulative incidences of relapse and clonal evolution were 9.0% [95% confidence interval (CI) = 4.2–16.0%] and 4.5% (95% CI = 1.4–10.6%), respectively. The 5-year overall survival (OS) and event-free survival rates were 83.7% (95% CI = 81.1–86.0%) and 50.4% (95% CI = 47.1–53.5%), respectively. Conclusion: p-ATG combined with CsA for the treatment of AA is effective and safe, and p-ATG can be used as an alternative ATG preparation for the standard IST regimen in areas in which h-ATG is not available.
    Type of Medium: Online Resource
    ISSN: 2040-6207 , 2040-6215
    URL: Article
    DOI: 10.1177/20406207221146031
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2585183-4
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  • 8
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    Cooperative Networking towards Maritime Cyber Physical Systems
    SAGE Publications ; 2016
    In:  International Journal of Distributed Sensor Networks Vol. 12, No. 3 ( 2016-03-01), p. 3906549-
    Details
    In: International Journal of Distributed Sensor Networks, SAGE Publications, Vol. 12, No. 3 ( 2016-03-01), p. 3906549-
    Abstract: An innovative paradigm named Cooperative Cognitive Maritime Cyber Physical Systems (CCMCPSs) is developed to achieve high-speed and low-cost communication services. The analysis of the available white space at sea, as well as the framework, is presented. Specifically, a bilevel game with two stages of PUs-to-SUs (primary users to secondary users) and SUs-to-SUs is proposed, to address the resource allocation issue of Decode-and-Forward (DF) relay mode with maximal-ratio combining (MRC) receiving mode in destination. Stackelberg game with priority is employed between PU and SUs, while a symmetrical system model is considered among SUs-to-SUs. The game theoretic procedure that converges to Nash equilibrium based on the utility and payoff function is illustrated. Simulation results demonstrate that our proposed strategy could effectively increase the throughput as well as the payoffs of the system.
    Type of Medium: Online Resource
    ISSN: 1550-1477 , 1550-1477
    URL: Article
    DOI: 10.1155/2016/3906549
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2192922-1
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  • 9
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    Political Efficacy and the Use of Local and National News Media Among Undecided Voters in a Swing State : A Study of General Population Voters and First-Time College Student Voters
    SAGE Publications ; 2013
    In:  Electronic News Vol. 7, No. 4 ( 2013-12), p. 204-222
    Details
    In: Electronic News, SAGE Publications, Vol. 7, No. 4 ( 2013-12), p. 204-222
    Abstract: This survey examined undecided voters’ local and national news media use in relation to political efficacy. Results suggest that online media use was the strongest predictor for political efficacy, creating a self-selection polarized trend of political use of media. Local election efficacy was higher than national election efficacy. Over one half of the first-time voters and general population voters believed their vote could change the outcome of the election. Social media only had an impact on first-time voters’ political efficacy. No liberals could be found in undecided voters among the general population voters. Television was the most important medium for campaign information for the general population, while the internet was the most important medium for first-time voters. The result signified the diminishing role of newspapers as an important source of campaign information.
    Type of Medium: Online Resource
    ISSN: 1931-2431 , 1931-244X
    URL: Article
    DOI: 10.1177/1931243113515678
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2428079-3
    SSG: 3,5
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  • 10
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    Stereo-crossed ablation guided by stereoelectroencephalography for epilepsy: comprehensive coagulations via a network of multi-electrodes
    SAGE Publications ; 2020
    In:  Therapeutic Advances in Neurological Disorders Vol. 13 ( 2020-01), p. 175628642092865-
    Details
    In: Therapeutic Advances in Neurological Disorders, SAGE Publications, Vol. 13 ( 2020-01), p. 175628642092865-
    Abstract: Introducing multiple different stereoelectroencephalography electrodes in a three-dimensional (3D) network to create a 3D-lesioning field or stereo-crossed radiofrequency thermocoagulation (scRF-TC) might create larger lesioning size; however, this has not been quantified to date. This study aimed to quantify the configurations essential for scRF-TC. Methods: By using polyacrylamide gel (PAG), we investigated the effect of electrode conformation (angled/parallel/multiple edges) and electrode distance of creating an electrode network. Volume, time, and temperature were analyzed quantitatively with magnetic resonance imaging, video analysis, and machine learning. A network of electrodes to the pathological left area 47 was created in a patient; the seizure outcome and coverage range were further observed. Results: After the compatibility test between the PAG and brain tissue, the sufficient distance of contacts (from different electrodes) for confluent lesioning was 7 mm with the PAG. Connection to the lesioning field could be achieved even with a different arrangement of electrodes. One contact could achieve at least six connections with different peripheral contacts. Coagulation with a network of electrodes can create more significant lesioning sizes, 1.81–2.12 times those of the classic approaches. The confluent lesioning field created by scRF-TC had a volume of 38.7 cm 3 ; the low metabolic area was adequately covered. The representative patient was free of seizures throughout the 12-month follow up. Conclusion: Lesioning with electrodes in a network manner is practical for adequate 3D coverage. A secondary craniotomy could be potentially prevented by combining both monitoring and a large volume of lesions.
    Type of Medium: Online Resource
    ISSN: 1756-2864 , 1756-2864
    URL: Article
    DOI: 10.1177/1756286420928657
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2442245-9
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