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  • 1
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    Online Resource
    Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice
    SAGE Publications ; 2020
    In:  Therapeutic Advances in Chronic Disease Vol. 11 ( 2020-01), p. 204062232097483-
    Details
    In: Therapeutic Advances in Chronic Disease, SAGE Publications, Vol. 11 ( 2020-01), p. 204062232097483-
    Abstract: Chronic exposure to excess glucocorticoids is frequently associated with a specific cardiomyopathy. Empagliflozin, a sodium-glucose cotransporter 2 (SGLT2) inhibitor, has beneficial effects as it aids in the reduction of heart failure and cardiovascular mortality in hospitalized patients. The aim of this study was to investigate the effects of empagliflozin on chronic hypercortisolism-induced myocardial fibrosis and myocardial dysfunction in mice. Methods: Male C57BL/6J mice (6 weeks old) were randomized to control, corticosterone (CORT), and empagliflozin + CORT groups. After 4 weeks of administration, heart structure and function were evaluated by echocardiography, and peripheral blood and tissue samples were collected. Expressions of Ccl2, Itgax, Mrc1, and Adgre1 mRNA in heart tissue were evaluated by RT-PCR, and signal transducer and activator of transcription 3 (STAT3) and Toll-like receptor 4 (TLR4) protein expression were analyzed by Western blotting. Results: Empagliflozin effectively reduced body weight, liver triglyceride, visceral adipose volume, and uric acid in CORT-treated mice. Left ventricular hypertrophy and cardiac dysfunction were improved significantly, phosphorylated STAT3 and TLR4 were alleviated, and macrophage infiltration in the myocardium was inhibited after administration of empagliflozin in CORT-treated mice. Conclusion: Empagliflozin has beneficial effects on specific cardiomyopathy associated with CORT, and the results provide new evidence that empagliflozin might be a potential drug for the prevention of this disease.
    Type of Medium: Online Resource
    ISSN: 2040-6223 , 2040-6231
    URL: Article
    DOI: 10.1177/2040622320974833
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
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  • 2
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    Genipin-crosslinked O-carboxymethyl chitosan–gum Arabic coacervate as a pH-sensitive delivery system and microstructure characterization
    SAGE Publications ; 2016
    In:  Journal of Biomaterials Applications Vol. 31, No. 2 ( 2016-08), p. 193-204
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 31, No. 2 ( 2016-08), p. 193-204
    Abstract: The possibility of genipin-crosslinked O-carboxymethyl chitosan–gum Arabic coacervate as a pH-sensitive delivery vehicle was investigated. O-carboxymethyl chitosan–gum Arabic coacervates separated in pH 3.0, 4.5, and 6.0 were crosslinked by genipin for different durations and the crosslinked products were subjected to crosslinking degree, swelling behavior, bovine serum albumin release profile, and microstructure characterization. Genipin-crosslinking greatly improved the stability of the coacervates against the simulated gastric solution and created certain pH-sensitivity. The coacervates displayed higher swelling ratios in the simulated gastric solution than in the simulated intestine and colon solutions; meanwhile, the coacervates prepared in pH 4.5 and 6.0 swelled more severely than the complex separated in pH 3.0. Nevertheless, the bovine serum albumin release in the simulated gastric solution from the microcapsules prepared in pH 6.0 was much lower than those prepared in pH 4.5 and 3.0, whose cumulative release percentages in the three simulated solutions were 17.14%, 55.23%, and 79.79%, respectively, in crosslinking duration 2 h. X-ray diffraction, scanning electron microscopy, and transmission electron microscopy analysis revealed that genipin-crosslinking improved the regularity and compactness of coacervate structure, whereas confocal laser scanning microscopy observation indicated that O-carboxymethyl chitosan content was possibly the major reason for the different swelling and bovine serum albumin release behavior of the coacervates. It was concluded that the genipin-crosslinked O-carboxymethyl chitosan–gum Arabic coacervate was a potential intestine-targeted delivery system and its delivery performance could be tailored by varying the crosslinking degree and coacervation acidity.
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328216651393
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2072559-0
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  • 3
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    Circulating tumor cells dynamics during chemotherapy predict survival and response in advanced non-small-cell lung cancer patients
    SAGE Publications ; 2023
    In:  Therapeutic Advances in Medical Oncology Vol. 15 ( 2023-01), p. 175883592311678-
    Details
    In: Therapeutic Advances in Medical Oncology, SAGE Publications, Vol. 15 ( 2023-01), p. 175883592311678-
    Abstract: Circulating tumor cells (CTCs) are prognostic biomarker in non-small-cell lung cancer (NSCLC). CTCs could also be used as predictor of efficacy of systemic treatments in advanced NSCLC. Objectives: We described the dynamic changes of CTCs during first-line platinum-based chemotherapy in advanced NSCLC and clarified the correlation between CTC counts and efficacy of chemotherapy. Design: Chemotherapy is administered and blood specimens are collected at four time points from baseline to disease progression for CTC detection. Methods: This multicenter prospective study enrolled patients with previously untreated stage III or IV NSCLC fit for standard platinum-based chemotherapy. Bloods were sampled as per standard operating procedures at baseline, cycle 1 and cycle 4 of chemotherapy, and at disease progression for CTC analysis using the CellSearch system. Results: Among 150 patients enrolled, median overall survival (OS) was 13.8, 8.4, and 7.9 months in patients with CTC − , KIT − CTC, and KIT + CTC at baseline ( p = 0.002). Patients with persistent negative CTC (46.0%) had longer progression-free survival [5.7 months, 95% confidence interval (CI): 5.0–6.5 versus 3.0 months, 0.6–5.4; hazard ratio (HR): 0.34, 95% CI: 0.18–0.67) and OS (13.1 months, 10.9–15.3 versus 5.6 months, 4.1–7.1; HR: 0.17, 0.08–0.36) compared with patients with persistent positive CTC (10.7%), which was not impacted by chemotherapy. Chemotherapy decreased CTC from 36.0% (54/150) to 13.7% (13/95). Conclusions: CTC persistent presence during treatment represents poor prognosis and resistance to chemotherapy in advanced NSCLC. Chemotherapy could effectively eliminate CTCs. Molecular characterization and the functionalization of CTC will be warranted for further intensive investigation. Trial registration: NCT01740804.
    Type of Medium: Online Resource
    ISSN: 1758-8359 , 1758-8359
    URL: Article
    DOI: 10.1177/17588359231167818
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2503443-1
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  • 4
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    Flow Cytometry Method Analysis of Apoptosis : No Significant Difference Between EDTA and EDTA-free Trypsin Treatment Procedure
    SAGE Publications ; 2015
    In:  Technology in Cancer Research & Treatment Vol. 14, No. 2 ( 2015-04), p. 237-241
    Details
    In: Technology in Cancer Research & Treatment, SAGE Publications, Vol. 14, No. 2 ( 2015-04), p. 237-241
    Abstract: Flow cytometry method (FCM) is a generally accepted tool to analyze apoptosis. Although apoptosis assay kit was applied by many companies, the manufacturers were not consistent with whether using Trypsin with EDTA to collect the adherent cells. In another words, the influence of EDTA on apoptotic ratio is not clear. In this work, we compared the proportion of apoptotic cells with EDTA or EDTA-free Trypsin treatment by FCM. We concluded that Trypsin with or without EDTA has little influence on the proportion of apoptotic cells. In addition, we found that the ratio of necrosis and apoptosis was different in cells collected by scraping. WAVE2 protein was analyzed as a typical example for movement related protein. WAVE2 expression is elevated in the EDTA Trypsin treated group, compared with EDTA-free Trypsin treatment and scrapping group.
    Type of Medium: Online Resource
    ISSN: 1533-0346 , 1533-0338
    URL: Article
    DOI: 10.7785/tcrt.2012.500406
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2146365-7
    detail.hit.zdb_id: 2220436-2
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  • 5
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    Sodium Tanshinone IIA Sulfonate Attenuates Tumor Oxidative Stress and Promotes Apoptosis in an Intermittent Hypoxia Mouse Model
    SAGE Publications ; 2020
    In:  Technology in Cancer Research & Treatment Vol. 19 ( 2020-01-01), p. 153303382092807-
    Details
    In: Technology in Cancer Research & Treatment, SAGE Publications, Vol. 19 ( 2020-01-01), p. 153303382092807-
    Abstract: Intermittent hypoxia, a significant feature of obstructive sleep apnea, has pro-tumorigenic effects. Here, we investigated the effect of sodium tanshinone IIA sulfonate on oxidative stress and apoptosis in a mouse model of Lewis lung carcinoma with intermittent hypoxia. Methods: Mice were randomly assigned to normoxia (control), normoxia plus sodium tanshinone IIA sulfonate (control + sodium tanshinone IIA sulfonate), intermittent hypoxia, and intermittent hypoxia + sodium tanshinone IIA sulfonate groups. Intermittent hypoxia administration lasted 5 weeks in the intermittent hypoxia groups. Lewis lung carcinoma cells were injected into the right flank of each mouse after 1 week of intermittent hypoxia exposure. Sodium tanshinone IIA sulfonate was injected intraperitoneally in the control + sodium tanshinone IIA sulfonate and intermittent hypoxia + sodium tanshinone IIA sulfonate groups. Tumor oxidative stress was evaluated by detection of malondialdehyde and superoxide dismutase. The apoptosis of tumor cells was evaluated by the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay as well as by Western blot analysis of B-cell lymphoma 2-associated X protein and cleaved caspase-3 expression. Additionally, the expression of hypoxia-induced factor-1α, nuclear factor erythroid 2-related factor 2, and nuclear factor kappa B was also evaluated by Western blot. Results: Compared with the control group, the intermittent hypoxia treatment significantly increased Lewis lung carcinoma tumor growth and oxidative stress (serum malondialdehyde) but decreased serum levels of SOD and pro-apoptotic markers (terminal deoxynucleotidyl transferase dUTP nick-end labeling staining, B-cell lymphoma 2-associated X protein, and cleaved caspase-3). These changes were significantly attenuated by intraperitoneal injection of sodium tanshinone IIA sulfonate. Lower nuclear factor erythroid 2-related factor 2 and higher nuclear factor kappa B levels in the intermittent hypoxia group were clearly reversed by sodium tanshinone IIA sulfonate treatment. In addition, sodium tanshinone IIA sulfonate administration decreased the high expression of hypoxia-induced factor-1α induced by intermittent hypoxia. Conclusion: Intermittent hypoxia treatment resulted in high oxidative stress and low apoptosis in Lewis lung carcinoma–implanted mice, which could be attenuated by sodium tanshinone IIA sulfonate administration possibly through a mechanism mediated by the nuclear factor erythroid 2-related factor 2/nuclear factor kappa B signaling pathway.
    Type of Medium: Online Resource
    ISSN: 1533-0346 , 1533-0338
    URL: Article
    DOI: 10.1177/1533033820928073
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2146365-7
    detail.hit.zdb_id: 2220436-2
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  • 6
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    The gastroprotective effect of pogostone from Pogostemonis Herba against indomethacin-induced gastric ulcer in rats
    SAGE Publications ; 2016
    In:  Experimental Biology and Medicine Vol. 241, No. 2 ( 2016-01), p. 193-204
    Details
    In: Experimental Biology and Medicine, SAGE Publications, Vol. 241, No. 2 ( 2016-01), p. 193-204
    Abstract: Pogostemonis Herba, known as “Guang-Huo-Xiang” in Chinese, has been widely used in the treatment of gastrointestinal dysfunction. Pogostone is one of the major constituents of Pogostemonis Herba. The aim was to scientifically evaluate the possible gastroprotective effect and the underlying mechanisms of pogostone against indomethacin-induced gastric ulcer in rats. Rats were orally treated with vehicle, lansoprazole (30 mg/kg) or pogostone (10, 20 and 40 mg/kg) and subsequently exposed to acute gastric lesions induced by indomethacin. Gross evaluation, histological observation, gastric mucosal superoxide dismutase activity, glutathione content, catalase activity, malonaldehyde level and prostaglandin E2 production were performed. Immunohistochemistry and reverse transcription polymerase chain reaction for cyclooxygenase-1 and cyclooxygenase-2, as well as terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling assay, immunohistochemistry for heat-shock protein 70, B-cell lymphoma-2 and Bax were conducted. Results indicated that rats pretreated with pogostone showed remarkable protection from the gastric mucosa damage compared to vehicle-treated rats based on the ulcer index and inhibition percentage. Histologically, oral administration of pogostone resulted in observable improvement of gastric injury, characterized by reduction of necrotic lesion, flattening of gastric mucosa and alleviation of submucosal edema with hemorrhage. Pogostone pretreatment significantly raised the depressed activities of superoxide dismutase, glutathione and catalase, while reduced the elevated malonaldehyde level compared with indomethacin-induced group. Pogostone-pretreated group induced a significant increase in gastric mucosal prostaglandin E2 level and obvious up-regulation of protein levels and mRNA expressions of cyclooxygenase-1 and cyclooxygenase-2. Furthermore, antiapoptotic effect of pogostone was verified by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling assay, and the apoptotic process triggered by pogostone involved the up-expression of heat-shock protein70 and B-cell lymphoma-2 protein, and suppression of Bax protein expressions in the ulcerated tissues. It is speculated that the gastroprotective effect of pogostone against indomethacin-induced gastric ulceration might be associated with its stimulation of cyclooxygenase-mediated prostaglandin E2, antioxidant and antiapoptotic effect.
    Type of Medium: Online Resource
    ISSN: 1535-3702 , 1535-3699
    URL: Article
    DOI: 10.1177/1535370215600099
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2020856-X
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  • 7
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    Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections
    SAGE Publications ; 2016
    In:  Experimental Biology and Medicine Vol. 241, No. 14 ( 2016-08), p. 1551-1558
    Details
    In: Experimental Biology and Medicine, SAGE Publications, Vol. 241, No. 14 ( 2016-08), p. 1551-1558
    Abstract: We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.
    Type of Medium: Online Resource
    ISSN: 1535-3702 , 1535-3699
    URL: Article
    DOI: 10.1177/1535370216647128
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2020856-X
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  • 8
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    Thrombocytopenia in 737 adult intensive care unit patients: A real-world study of associated factors, drugs, platelet transfusion, and clinical outcome
    SAGE Publications ; 2020
    In:  SAGE Open Medicine Vol. 8 ( 2020-01), p. 205031212095890-
    Details
    In: SAGE Open Medicine, SAGE Publications, Vol. 8 ( 2020-01), p. 205031212095890-
    Abstract: We aimed to identify and represent factors associated with thrombocytopenia in intensive care unit, especially the pathogens and drugs related to severe and extremely thrombocytopenia. Then, we aim to compare the mortality of platelet transfusion and non-transfusion in patients with different degrees of thrombocytopenia. Methods: We identified all thrombocytopenic patients in intensive care unit by using platelet-specific values and then extracted electronic health records from our Hospital Information System. Data were statistically analyzed with t test, chi-square test, and logistic regression. Results: We found that infections (32.7%) were the most frequent cause associated with thrombocytopenia, followed by sepsis shock (3.93%) and blood loss (2.99%). Meanwhile, antifungals ( p = 0.002) and bacterial infection ( p = 0.037) were associated with severe and extremely severe thrombocytopenia. Finally, we found that the mortality of platelet transfusion and non-transfusion in patients was statistically significant for patients with platelet counts between 30 and 49/nL (χ 2  = 9.719, p = 0.002). Conclusion: Infection and sepsis emerged as two primary factors associated with thrombocytopenia in intensive care unit. Meanwhile, antifungals and bacterial infection were associated with platelet counts less than 49/nL. Finally, platelet transfusion may be associated with reduced mortality in patients with platelet counts between 30 and 49/nL.
    Type of Medium: Online Resource
    ISSN: 2050-3121 , 2050-3121
    URL: Article
    DOI: 10.1177/2050312120958908
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2735399-0
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  • 9
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    Isoprenylated Phenolics from Roots of Artocarpus heterophyllus
    SAGE Publications ; 2017
    In:  Natural Product Communications Vol. 12, No. 6 ( 2017-06), p. 1934578X1701200-
    Details
    In: Natural Product Communications, SAGE Publications, Vol. 12, No. 6 ( 2017-06), p. 1934578X1701200-
    Abstract: Thirteen compounds were isolated from Artocarpus heterophyllus, including one new di-isoprenylated stilbene, (±)-artoheterin A (1), and twelve known isoprenylated phenolics, artocarbene (2), 3′,5′,2,4-tetrahydroxy-4′-(3-methyl-2-butenyl) stilbene (3), cudrastilbene (4), 3-(3-methyl-2-butenyl) luteolin (5), 5,7,2′,4′-tetrahydroxy-6-(3-methyl-2-butenyl) flavone (6), 6-(3-methyl-2-butenyl) apigenin (7), artochamin B (8), 5′-hydroxycudraflavone A (9), artonins A and B (10–11), heterophyllin (12), and 8-(3-methyl-2-butenyl)-6,7-dihydroxycoumarin (13). The structures of these compounds were identified by spectroscopic methods. All isolated compounds were screened for their inhibitory abilities against cathepsin K (CatK). Of them, compounds 1–4 and 8–13 were found to have suppression capabilities against CatK with IC 50 values ranging from 1.9 to 73.7 μM.
    Type of Medium: Online Resource
    ISSN: 1934-578X , 1555-9475
    URL: Article
    DOI: 10.1177/1934578X1701200622
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2430442-6
    SSG: 15,3
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  • 10
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    Study on the performance of the acetylated bamboo fiber/PBS composites by molecular dynamics simulation
    SAGE Publications ; 2016
    In:  Journal of Composite Materials Vol. 50, No. 7 ( 2016-03), p. 995-1003
    Details
    In: Journal of Composite Materials, SAGE Publications, Vol. 50, No. 7 ( 2016-03), p. 995-1003
    Abstract: A hypothesis, the mechanical properties, interfacial linkage, crystallization, and thermal stability between acetylated bamboo fiber (Ac-BF) and poly (butylene succinate) (PBS) matrix were significantly affected by the interactions between Ac-BF and PBS composites, was proposed based on the molecular dynamics simulation technique. Thereafter, SEM, XRD, TG and mechanical testing were employed to certify the above hypothesis. However, Ac-BF/PBS composite showed better stability than that of BF/PBS due to the lower mixing energy and Flory-Huggins interaction parameters ( χ). Further, strong van der Waals (VdW) interaction, hydrogen-bond interaction and physical adsorption existed between functional groups of composites. The tensile strength and the initial decomposition temperature ( T d-5% ) of Ac-BF/PBS increased by 18% and 12.2℃, respectively, due to the fiber acetoxylation.
    Type of Medium: Online Resource
    ISSN: 0021-9983 , 1530-793X
    URL: Article
    DOI: 10.1177/0021998315615690
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 160490-9
    detail.hit.zdb_id: 2081924-9
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