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  • SAGE Publications  (32)
  • 1
    Online-Ressource
    Online-Ressource
    Docosahexaenoic Acid Promotes Dopaminergic Differentiation in Induced Pluripotent Stem Cells and Inhibits Teratoma Formation in Rats with Parkinson-Like Pathology
    SAGE Publications ; 2012
    In:  Cell Transplantation Vol. 21, No. 1 ( 2012-02), p. 313-332
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 21, No. 1 ( 2012-02), p. 313-332
    Kurzfassung: Parkinson's disease (PD) is a neurodegenerative disorder characterized by the degeneration of dopaminergic (DA) neurons in the midbrain. Induced pluripotent stem (iPS) cells have shown potential for differentiation and may become a resource of functional neurons for the treatment of PD. However, teratoma formation is a major concern for transplantation-based therapies. This study examined whether functional neurons could be efficiently generated from iPS cells using a five-step induction procedure combined with docosahexaenoic acid (DHA) treatment. We demonstrated that DHA, a ligand for the RXR/Nurr1 heterodimer, significantly activated expression of the Nurr1 gene and the Nurr1-related pathway in iPS cells. DHA treatment facilitated iPS differentiation into tyrosine hydroxylase (TH)-positive neurons in vitro and in vivo and functionally increased dopamine release in transplanted grafts in PD-like animals. Furthermore, DHA dramatically upregulated the endogenous expression levels of neuroprotective genes ( Bcl-2, Bcl-xl, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor) and protected against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis in iPS-derived neuronal precursor cells. DHA-treated iPS cells significantly improved the behavior of 6-hydroxydopamine (6-OHDA)-treated PD-like rats compared to control or eicosapentaenoic acid-treated group. Importantly, the in vivo experiment suggests that DHA induces the differentiation of functional dopaminergic precursors and improves the abnormal behavior of 6-OHDA-treated PD-like rats by 4 months after transplantation. Furthermore, we found that DHA treatment in iPS cell-grafted rats significantly downregulated the mRNA expression of embryonic stem cell-specific genes (Oct-4 and c-Myc) in the graft and effectively blocked teratoma formation. Importantly, 3 Tesla-magnetic resonance imaging and ex vivo green fluorescence protein imaging revealed that no teratomas were present in transplanted grafts of DHA-treated iPS-derived DA neurons 4 months after implantation. Therefore, our data suggest that DHA plays a crucial role in iPS differentiation into functional DA neurons and that this approach could provide a novel therapeutic approach for PD treatment.
    Materialart: Online-Ressource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368911X580572
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2012
    ZDB Id: 2020466-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Stem Cell Therapy for Corneal Regeneration Medicine and Contemporary Nanomedicine for Corneal Disorders
    SAGE Publications ; 2015
    In:  Cell Transplantation Vol. 24, No. 10 ( 2015-10), p. 1915-1930
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 24, No. 10 ( 2015-10), p. 1915-1930
    Kurzfassung: The ocular surface is the outermost part of the visual system that faces many extrinsic or intrinsic threats, such as chemical burn, infectious pathogens, thermal injury, Stevens–Johnson syndrome, ocular pemphegoid, and other autoimmune diseases. The cornea plays an important role in conducting light into the eyes and protecting intraocular structures. Several ocular surface diseases will lead to the neovascularization or conjunctivalization of corneal epithelium, leaving opacified optical media. It is believed that some corneal limbal cells may present stem cell-like properties and are capable of regenerating corneal epithelium. Therefore, cultivation of limbal cells and reconstruction of the ocular surface with these limbal cell grafts have attracted tremendous interest in the past few years. Currently, stem cells are found to potentiate regenerative medicine by their capability of differentiation into multiple lineage cells. Among these, the most common cell sources for clinical use are embryonic, adult, and induced stem cells. Different stem cells have varied specific advantages and limitations for in vivo and in vitro expansion. Other than ocular surface diseases, culture and transplantation of corneal endothelial cells is another major issue for corneal decompensation and awaits further studies to find out comprehensive solutions dealing with nonregenerative corneal endothelium. Recently, studies of in vitro endothelium culture and ρ-associated kinase (ROCK) inhibitor have gained encouraging results. Some clinical trials have already been finished and achieved remarkable vision recovery. Finally, nanotechnology has shown great improvement in ocular drug delivery systems during the past two decades. Strategies to reconstruct the ocular surface could combine with nanoparticles to facilitate wound healing, drug delivery, and even neovascularization inhibition. In this review article, we summarized the major advances of corneal limbal stem cells, limbal stem cell deficiency, corneal endothelial cell culture/transplantation, and application of nanotechnology on ocular surface reconstruction. We also illustrated potential applications of current knowledge for the future treatment of ocular surface diseases.
    Materialart: Online-Ressource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368914X685744
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2015
    ZDB Id: 2020466-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Characteristics of integrated 18 F-FDG PET/CT in pulmonary cryptococcosis
    SAGE Publications ; 2009
    In:  Acta Radiologica Vol. 50, No. 4 ( 2009-05), p. 374-378
    Details
    In: Acta Radiologica, SAGE Publications, Vol. 50, No. 4 ( 2009-05), p. 374-378
    Kurzfassung: Background: Pulmonary cryptococcosis is an uncommon cause of pulmonary nodules found by 18 F-fluorodeoxyglucose positron emission tomography/computed tomography ( 18 F-FDG PET/CT) scans. It is rarely reported but may mislead interpretation. Purpose: To describe the 18 F-FDG PET/CT findings of pulmonary cryptococcosis. Material and Methods: The 18 F-FDG PET/CT images of seven patients with pulmonary cryptococcosis were evaluated. Results: The 18 F-FDG PET/CT exams showed single or multiple nodular lesions. The standardized uptake values (SUV) in early images varied significantly for the seven patients (ranging from 2.2 to 11.6). Delayed SUVs showed significant increases in four patients. Conclusion: Pulmonary cryptococcosis mimics primary or metastatic lung cancer on 18 F-FDG PET/CT scan. Tissue confirmation should be considered for any suspicious pulmonary nodules found on 18 F-FDG PET/CT scan with an SUV score higher than 2.5, in order to avoid overdiagnosis or overstaging.
    Materialart: Online-Ressource
    ISSN: 0284-1851 , 1600-0455
    URL: Article
    DOI: 10.1080/02841850902756532
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2009
    ZDB Id: 2024579-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    Paradox of Accessory Pathway Block After Radiofrequency Ablation in Patients with the Wolff-Parkinson-White Syndrome
    SAGE Publications ; 1996
    In:  Angiology Vol. 47, No. 11 ( 1996-11), p. 1061-1071
    Details
    In: Angiology, SAGE Publications, Vol. 47, No. 11 ( 1996-11), p. 1061-1071
    Kurzfassung: Although pacing technique has demonstrated that the most common site of conduction block in a manifest accessory pathway (AP) was between the AP and the ventricle, most of the block sites have been found to be between the atrium and AP after successful radiofrequency ablation. Furthermore, the block site in a concealed AP after successful radiofrequency catheter ablation has not been reported in the literature, and compar isons between a manifest and concealed AP have not been performed. This study included 219 consecutive patients undergoing successful radiofrequency catheter ablation of a single AP. AP potential was recorded at the successful target site in 76 of 92 (82.6%) patients with manifest APs, and in 99 of 127 (77.9%) patients with concealed APs. All the left-sided APs (including left posteroseptal APs) were ablated by a ventricular approach, and right-sided APs (including anteromidseptal and right posteroseptal APs) were ablated by an atrial approach. The site of conduction block was determined by analyzing and comparing the local electrograms recorded before and after radiofrequency ablation at successful ablation sites. Conduction block of manifest APs was between the atrial-AP (A-AP) in 69 patients (75%) and between the AP-ventricle (AP-V) interface in 7 patients (7.6%), whereas the conduction block of concealed APs occurred between the AP-V in 90 patients (70.9%) and between the A-AP interface in 9 patients (7.1%). Neither the preab lation electrogram nor electrophysiologic characteristics of APs predicted the site of conduction block. Furthermore, neither the location of the APs nor the position of the ablation catheter affected the block site. It was concluded that the most common site of conduction block during successful radiofrequency catheter ablation of a manifest and concealed AP was between the A-AP and AP-V interface, respectively, and the impedance mismatch theory explained only part of the findings.
    Materialart: Online-Ressource
    ISSN: 0003-3197 , 1940-1574
    URL: Article
    DOI: 10.1177/000331979604701106
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 1996
    ZDB Id: 2065911-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    Characterization of Axon Formation in the Embryonic Stem Cell-Derived Motoneuron
    SAGE Publications ; 2011
    In:  Cell Transplantation Vol. 20, No. 4 ( 2011-05), p. 493-502
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 20, No. 4 ( 2011-05), p. 493-502
    Kurzfassung: The developing neural cell must form a highly organized architecture to properly receive and transmit nerve signals. Neural formation from embryonic stem (ES) cells provides a novel system for studying axonogenesis, which are orchestrated by polarity-regulating molecules. Here the ES-derived motoneurons, identified by HB9 promoter-driven green fluorescent protein (GFP) expression, showed characteristics of motoneuron-specific gene expression. In the majority of motoneurons, one of the bilateral neurites developed into an axon that featured with axonal markers, including Tau 1, vesicle acetylcholine transporter, and synaptophysin. Interestingly, one third of the motoneurons developed bi-axonal processes but no multiple axonal GFP cell was found. The neuronal polarity-regulating proteins, including the phosphorylated AKT and ERK, were compartmentalized into both of the bilateral axonal tips. Importantly, this aberrant axon morphology was still present after the engraftment of GFP + neurons into the spinal cord, suggesting that even a mature neural environment fails to provide a proper niche to guide normal axon formation. These findings underscore the necessity for evaluating the morphogenesis and functionality of neurons before the clinical trials using ES or somatic stem cells.
    Materialart: Online-Ressource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368910X536464
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2011
    ZDB Id: 2020466-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    Minimal Detectable Change and Clinically Important Difference of the Stroke Impact Scale in Stroke Patients
    SAGE Publications ; 2010
    In:  Neurorehabilitation and Neural Repair Vol. 24, No. 5 ( 2010-06), p. 486-492
    Details
    In: Neurorehabilitation and Neural Repair, SAGE Publications, Vol. 24, No. 5 ( 2010-06), p. 486-492
    Kurzfassung: Objectives. The purpose of this study was to establish the minimal detectable change (MDC) and clinically important differences (CIDs) of the physical domains of the Stroke Impact Scale (SIS) and to assess the proportions of patients’ change scores exceeding the MDC and CIDs after stroke rehabilitation. Methods. Seventy-four patients received 1 of 3 treatments for 3 weeks and underwent clinical assessment before and after treatment. The MDC was calculated from the standard error of measurement to indicate a real change with 95% confidence for individual patients (MDC 95 ). Anchor-based and distribution-based approaches were adopted to triangulate the ranges of minimal CIDs. The percentage of patients exceeding MDC 95 and minimal CIDs were also calculated. Results. The MDC 95 of the strength, activities of daily living/instrumental activities of daily living, mobility, and hand function subscales were 24.0, 17.3, 15.1, and 25.9, respectively. The respective minimal CIDs for these 4 subscales were 9.2, 5.9, 4.5, and 17.8 points, respectively, and the MDC 95 and CID proportions were 14% to 43%, 16% to 49%, 10% to 50%, and 23% to 64%, respectively. Conclusions . The change score of an individual patient has to reach 24.0, 17.3, 15.1, and 25.9 on the 4 subscales to indicate a true change. The mean change scores of a stroke group on the 4 subscales should reach 9.2, 5.9, 4.5, and 17.8 points to be regarded as clinically important changes. Future research with larger sample sizes is warranted to validate these estimates.
    Materialart: Online-Ressource
    ISSN: 1545-9683 , 1552-6844
    URL: Article
    DOI: 10.1177/1545968309356295
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2010
    ZDB Id: 2100545-X
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
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    Enriched Peripheral Blood-Derived Mononuclear Cells for Treating Knee Osteoarthritis
    SAGE Publications ; 2023
    In:  Cell Transplantation Vol. 32 ( 2023-01), p. 096368972211494-
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 32 ( 2023-01), p. 096368972211494-
    Kurzfassung: Osteoarthritis (OA) is a common chronic skeletal disease in the elderly. There is no effective therapy to reverse disease severity and knee OA (KOA) progression, particularly at the late stage. This study aims to examine the effect of peripheral blood-derived mononuclear cells (PBMNCs) on pain and motor function rescue in patients with Kellgren–Lawrence (KL) grade II to IV KOA. Participants received one intra-articular (IA) injection of autologous PBMNCs. The mononuclear cells were isolated from peripheral blood, enriched by a specialized medium (MoFi medium), and separated by Ficoll-Paque solution. The isolated and enriched PBMNCs could differentiate into M1 and M2 macrophages in vitro. The in vivo anti-inflammatory effect of the PBMNCs was similar to that of bone marrow mesenchymal stem cells, evaluated by complete Freund’s adjuvant-induced arthritis in rodents. A single-arm and open-label pilot study showed that patients’ knee pain and motor dysfunction were significantly attenuated after the cell transplantation, assessed by visual analogue scale (VAS) and Knee injury and Osteoarthritis Outcome Score (KOOS) at 6 and 12 months post-treatment. Notably, the therapeutic effect of the PBMNCs treatment can be stably maintained for 24 months, as revealed by the KOOS scores. These preclinical and pilot clinical data suggest that IA injection of MoFi-PBMNCs might serve as a novel medical technology to control the pain and the progress of KOA.
    Materialart: Online-Ressource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.1177/09636897221149445
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2023
    ZDB Id: 2020466-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
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    Application of computer vision in the automatic identification and classification of woven fabric weave patterns
    SAGE Publications ; 2010
    In:  Textile Research Journal Vol. 80, No. 20 ( 2010-12), p. 2144-2157
    Details
    In: Textile Research Journal, SAGE Publications, Vol. 80, No. 20 ( 2010-12), p. 2144-2157
    Kurzfassung: Traditionally, fabric texture identification is based on visual inspection. Recent studies have proposed automatic recognition, which utilizes computer vision to recognize the texture of different fabrics. In the recognition process, the fabric weave patterns are identified by the warp and weft floats. However, due to the optical environments and the appearance differences of fabrics and yarns, the stability and fault-tolerance of the computer vision method are yet to be improved. By using the fabric weave patterns image identification system, this study analyzed the fabric image to find out the warp and weft by the pixel gray-level cumulative values. It then cut out the image of the warp and weft floats to obtain the texture feature values, and used the Fuzzy C-Means (FCM) algorithm to identify the warp and weft floats. The identification results can derive the black-white digital image and the digital matrix of the fabric weave patterns. Finally, weaves classification is conducted based on the successfully trained two-stage Back-Propagation Neural Network. This two-stage neural network can be used to construct the computer vision system to recognize fabric texture, and to increase the system reliability and accuracy. This study used the first-order and second-order co-occurrence matrix, and confirmed that fabric patterns can be identified and classified accurately with this method.
    Materialart: Online-Ressource
    ISSN: 0040-5175 , 1746-7748
    URL: Article
    DOI: 10.1177/0040517510373630
    RVK:
    ZS 0001
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2010
    ZDB Id: 2209596-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
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    Isatis indigotica Induces Hepatocellular Cancer Cell Death via Caspase-Independent Apoptosis-Inducing Factor Translocation Apoptotic Pathway In Vitro and In Vivo
    SAGE Publications ; 2011
    In:  Integrative Cancer Therapies Vol. 10, No. 2 ( 2011-06), p. 201-214
    Details
    In: Integrative Cancer Therapies, SAGE Publications, Vol. 10, No. 2 ( 2011-06), p. 201-214
    Kurzfassung: Isatis indigotica is a biennial herbaceous cruciferous medical herb with antipyretic, antiviral, anti-inflammatory, and anti-endotoxin activity. This study explored the chemotherapeutic potential of I indigotica on human hepatoma cells and investigated the mechanism by which metabolites from I indigotica inhibit hepatoma cell growth. Antitumor activity was discovered in dried I indigotica leaf chloroform extracts (CEDLI). In nude mice xenotransplanted with human hepatoma cells, CEDLI supplementation inhibited tumor growth by ~40% compared with nonsupplemented animals without affecting body weight/food intake. CEDLI induced sub-G1 cell cycle arrest and apoptosis in hepatoma cells. Furthermore, CEDLI activates p53 and Bax, reduces Bcl-2 expression, and causes mitochondrial stress and the release of apoptosis-inducing factor into the cytosol followed by its translocation into the nucleus, resulting in hepatoma cell apoptosis. This study provides novel in vivo evidence of I indigotica’s antitumor activity. The chemotherapeutic activity against human hepatoma tumorigenesis was because of a distinguished caspase-independent apoptotic pathway.
    Materialart: Online-Ressource
    ISSN: 1534-7354 , 1552-695X
    URL: Article
    DOI: 10.1177/1534735410387420
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2011
    ZDB Id: 2101248-9
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
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    Online-Ressource
    Comparison of afatinib and erlotinib combined with bevacizumab in untreated stage IIIB/IV epidermal growth factor receptor-mutated lung adenocarcinoma patients: a multicenter clinical analysis study
    SAGE Publications ; 2022
    In:  Therapeutic Advances in Medical Oncology Vol. 14 ( 2022-01), p. 175883592211132-
    Details
    In: Therapeutic Advances in Medical Oncology, SAGE Publications, Vol. 14 ( 2022-01), p. 175883592211132-
    Kurzfassung: Although bevacizumab in combination with afatinib or erlotinib is an effective and safe first-line therapy for advanced epidermal growth factor receptor (EGFR)-mutated non-small-cell lung cancer (NSCLC), there are very few clinical data comparing afatinib and erlotinib combined with bevacizumab. We performed a retrospective multicenter analysis for the comparison of two combination therapies. Methods: Between May 2015 and October 2020, data of 135 stage IIIB/IV EGFR-mutated NSCLC patients receiving first-line afatinib or erlotinib combined with bevacizumab combination therapy in Linkou, Keelung, Chiayi, and Kaohsiung Chang Gung Memorial Hospitals were retrieved and retrospectively analyzed. Results: In all, 67 patients received afatinib plus bevacizumab, and 68 patients received erlotinib plus bevacizumab. Afatinib combined with bevacizumab had an objective response rate (ORR) of 82.1% and a disease control rate (DCR) of 97.0%, and the ORR and DCR were 83.8 and 95.6%, respectively, in the erlotinib combined with bevacizumab group ( p = 0.798 and p = 1.000). The median progression-free survival was 20.7 and 20.3 months for the afatinib plus bevacizumab group and the erlotinib plus bevacizumab group, respectively [hazard ratio (HR) = 1.02; 95% confidence interval (CI), 0.891–1.953; p = 0.167). The overall survival was 41.9 and 51.0 months for the afatinib plus bevacizumab group and erlotinib plus bevacizumab group, respectively (HR = 1.42; 95% CI, 0.829–2.436; p = 0.201). The secondary EGFR-T790M mutation rates after disease progression were 44% in the afatinib plus bevacizumab group and 58.8% in the erlotinib plus bevacizumab group ( p = 0.165). Skin toxicity was the most frequent treatment-related adverse event (AE) in both treatment groups. Diarrhea, an AE, occurred significantly more frequently in the afatinib plus bevacizumab group than in the erlotinib plus bevacizumab group ( p  〈  0.05). Conclusion: Afatinib combined with bevacizumab was equally as effective as erlotinib combined with bevacizumab for untreated advanced EGFR-mutated NSCLC. Prospective clinical studies that explore bevacizumab combined with afatinib or erlotinib for advanced EGFR-mutated NSCLC are warranted.
    Materialart: Online-Ressource
    ISSN: 1758-8359 , 1758-8359
    URL: Article
    DOI: 10.1177/17588359221113278
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2022
    ZDB Id: 2503443-1
    Standort Signatur Einschränkungen Verfügbarkeit
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