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  • SAGE Publications  (7)
  • 1
    Online Resource
    Online Resource
    The Bone Sialoprotein RGD Domain Modulates and Maintains Periodontal Development
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 10 ( 2022-09), p. 1238-1247
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 10 ( 2022-09), p. 1238-1247
    Abstract: Bone sialoprotein (gene: Ibsp; protein: BSP) is a multifunctional extracellular matrix protein present in bone, cementum, and dentin. Accumulating evidence supports BSP as a key regulator of mineralized tissue formation via evolutionarily conserved functional domains, including a C-terminal integrin-binding Arg-Gly-Asp (RGD) domain implicated in extracellular matrix–cell signaling. Ablation of Ibsp in mice ( Ibsp −/− ) results in impaired bone growth and mineralization and defective osteoclastogenesis, with effects in the craniofacial region including reduced acellular cementum formation, detachment of the periodontal ligament (PDL), alveolar bone hypomineralization, and severe periodontal breakdown. We hypothesized that BSP-RGD plays an important role in cementum and alveolar bone formation and mineralization, as well as periodontal function. This hypothesis was tested by replacing the RGD motif with a nonfunctional Lys-Ala-Glu (KAE) sequence in ( Ibsp KAE/KAE ) mice and OCCM.30 murine ( Ibsp KAE ) cementoblasts. The RGD domain was not critical for acellular or cellular cementum formation in Ibsp KAE/KAE mice. However, PDL volume and thickness were increased, and significantly more tartrate-resistant acid phosphatase–positive osteoclasts were found on alveolar bone surfaces of Ibsp KAE/KAE mice versus wild type mice. PDL organization was disrupted as indicated by picrosirius red stain, second harmonic generation imaging, dynamic mechanical analysis, and decreased asporin proteoglycan localization. In vitro studies implicated RGD functions in cell migration, adhesion, and mineralization, and this was confirmed by an ossicle implant model where cells lacking BSP-RGD showed substantial defects as compared with controls. In total, the BSP-RGD domain is implicated in periodontal development, though the scale and scope of changes indicated by in vitro studies indicate that other factors may partially compensate for and reduce the phenotypic severity of mice lacking BSP-RGD in vivo.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345221100794
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2057074-0
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  • 2
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    Online Resource
    Delivery of Alkaline Phosphatase Promotes Periodontal Regeneration in Mice
    SAGE Publications ; 2021
    In:  Journal of Dental Research Vol. 100, No. 9 ( 2021-08), p. 993-1001
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 100, No. 9 ( 2021-08), p. 993-1001
    Abstract: Factors regulating the ratio of pyrophosphate (PP i ) to phosphate (P i ) modulate biomineralization. Tissue-nonspecific alkaline phosphatase (TNAP) is a key promineralization enzyme that hydrolyzes the potent mineralization inhibitor PP i . The goal of this study was to determine whether TNAP could promote periodontal regeneration in bone sialoprotein knockout mice ( Ibsp −/− mice), which are known to have a periodontal disease phenotype. Delivery of TNAP was accomplished either systemically (through a lentiviral construct expressing a mineral-targeted TNAP-D 10 protein) or locally (through addition of recombinant human TNAP to a fenestration defect model). Systemic TNAP-D 10 delivered by intramuscular injection at 5 d postnatal (dpn) increased circulating alkaline phosphatase (ALP) levels in Ibsp −/− mice by 5-fold at 30 dpn, with levels returning to normal by 60 dpn when tissues were evaluated by micro–computed tomography and histology. Local delivery of recombinant human TNAP to fenestration defects in 5-wk-old wild type (WT) and Ibsp −/− mice did not alter long-term circulating ALP levels, and tissues were evaluated by micro–computed tomography and histology at postoperative day 45. Systemic and local delivery of TNAP significantly increased alveolar bone volume (20% and 37%, respectively) and cementum thickness (3- and 42-fold) in Ibsp −/− mice, with evidence for periodontal ligament attachment and bone/cementum marker localization. Local delivery significantly increased regenerated cementum and bone in WT mice. Addition of 100-μg/mL bovine intestinal ALP to culture media to increase ALP in vitro increased media P i concentration, mineralization, and Spp1 and Dmp1 marker gene expression in WT and Ibsp −/− OCCM.30 cementoblasts. Use of phosphonoformic acid, a nonspecific inhibitor of sodium P i cotransport, indicated that effects of bovine intestinal ALP on mineralization and marker gene expression were in part through P i transport. These findings show for the first time through multiple in vivo and in vitro approaches that pharmacologic modulation of P i /PP i metabolism can overcome periodontal breakdown and accomplish regeneration.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345211005677
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
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  • 3
    Online Resource
    Online Resource
    Ablation of Pyrophosphate Regulators Promotes Periodontal Regeneration
    SAGE Publications ; 2021
    In:  Journal of Dental Research Vol. 100, No. 6 ( 2021-06), p. 639-647
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 100, No. 6 ( 2021-06), p. 639-647
    Abstract: Biomineralization is regulated by inorganic pyrophosphate (PP i ), a potent physiological inhibitor of hydroxyapatite crystal growth. Progressive ankylosis protein (ANK) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) act to increase local extracellular levels of PP i , inhibiting mineralization. The periodontal complex includes 2 mineralized tissues, cementum and alveolar bone (AB), both essential for tooth attachment. Previous studies demonstrated that loss of function of ANK or ENPP1 (reducing PP i ) resulted in increased cementum formation, suggesting PP i metabolism may be a target for periodontal regenerative therapies. To compare the effects of genetic ablation of Ank, Enpp1, and both factors concurrently on cementum and AB regeneration, mandibular fenestration defects were created in Ank knockout ( Ank KO), Enpp1 mutant ( Enpp1 asj/asj ), and double KO (dKO) mice. Genetic ablation of Ank, Enpp1, or both factors increased cementum regeneration compared to controls at postoperative days (PODs) 15 and 30 ( Ank KO: 8-fold, 3-fold; Enpp1 asj/asj : 7-fold, 3-fold; dKO: 11-fold, 4-fold, respectively) associated with increased fluorochrome labeling and expression of mineralized tissue markers, dentin matrix protein 1 ( Dmp1/DMP1), osteopontin ( Spp1/OPN), and bone sialoprotein ( Ibsp/BSP). Furthermore, dKO mice featured increased cementum thickness compared to single KOs at POD15 and Ank KO at POD30. No differences were noted in AB volume between genotypes, but osteoblast/osteocyte markers were increased in all KOs, partially mineralized osteoid volume was increased in dKO versus controls at POD15 (3-fold), and mineral density was decreased in Enpp1 asj/asj and dKOs at POD30 (6% and 9%, respectively). Increased numbers of osteoclasts were present in regenerated AB of all KOs versus controls. These preclinical studies suggest PP i modulation as a potential and novel approach for cementum regeneration, particularly targeting ENPP1 and/or ANK. Differences in cementum and AB regeneration in response to reduced PP i conditions highlight the need to consider tissue-specific responses in strategies targeting regeneration of the entire periodontal complex.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/0022034520981854
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
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  • 4
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    Online Resource
    Multichannel Asynchronous Time-Resolving System for a Conventional FT-IR Spectrophotometer
    SAGE Publications ; 1999
    In:  Applied Spectroscopy Vol. 53, No. 5 ( 1999-05), p. 588-594
    Details
    In: Applied Spectroscopy, SAGE Publications, Vol. 53, No. 5 ( 1999-05), p. 588-594
    Abstract: A novel multichannel asynchronous time-resolving system with 16 time-resolving channels has been developed for conventional FT-IR spectrophotometers. The system enables measurement at 16 time delays simultaneously. This reduces the measuring time substantially and decreases possible damage of a sample by the stimulus. Moreover, simultaneous time resolving by the multichannel system improves the reliability of the data. As a performance test of the system, application to the study of reorientation dynamics in a ferroelectric liquid crystal is reported.
    Type of Medium: Online Resource
    ISSN: 0003-7028 , 1943-3530
    URL: Article
    DOI: 10.1366/0003702991946956
    RVK:
    VA 2367
    Language: English
    Publisher: SAGE Publications
    Publication Date: 1999
    detail.hit.zdb_id: 1474251-2
    SSG: 11
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  • 5
    Online Resource
    Online Resource
    Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
    SAGE Publications ; 1996
    In:  Journal of Histochemistry & Cytochemistry Vol. 44, No. 8 ( 1996-08), p. 845-853
    Details
    In: Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 44, No. 8 ( 1996-08), p. 845-853
    Abstract: We examined interspecies reactivities of eight anti-human monocyte/macrophage monoclonal antibodies (MAbs), Am-3K, PM-2K, X4, X14, Ber-MAC3, GHI/61, EBM/11, and KP1, with various animal tissues including rats, guinea pigs, rabbits, cats, dogs, goats, pigs, bovines, horses, and monkeys. All MAbs recognized monkey macrophages. Pig macrophages were detected by most MAbs except for EBM/11 and KP1. Of the eight antibodies, AM-3K showed the widest interspecies reactivity. It reacted with macrophages of all animal species examined, except for rats. Western blot analysis revealed a similarity in the antigens recognized by AM-3K among guinea pigs, rabbits, and humans. Other anti-human MAbs demonstrated distinct reactive patterns against macrophages in animals. The immunostaining patterns of all of these MAbs in animal tissues were similar to those found in humans, although some MAbs, such as AM-3K, EBM/11, and X4, displayed more restricted reactivity in animals than in humans. These results indicate that some anti-human monocyte/macrophage MAbs are also available for immunohistochemical detection of monocyte/macrophages in animal tissues. Among them, AM-3K is considered to be the most useful MAb for identifying macrophages in various tissues of animals.
    Type of Medium: Online Resource
    ISSN: 0022-1554 , 1551-5044
    URL: Article
    DOI: 10.1177/44.8.8756757
    Language: English
    Publisher: SAGE Publications
    Publication Date: 1996
    detail.hit.zdb_id: 1421306-0
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    A basic study on a urea-selective catalytic reduction system for a medium-duty diesel engine
    SAGE Publications ; 2005
    In:  International Journal of Engine Research Vol. 6, No. 1 ( 2005-02-01), p. 11-19
    Details
    In: International Journal of Engine Research, SAGE Publications, Vol. 6, No. 1 ( 2005-02-01), p. 11-19
    Abstract: NO x conversion performance of a urea-selective catalytic reduction (SCR) system comprising V 2 O 5 /TiO 2 catalyst under steady state operating conditions of an 8-litre, common-rail turbo direct injection (TDI) diesel engine was investigated. It was shown that the urea-SCR system achieves 70–90 per cent NO x conversion under medium and high load conditions at 1440 r/min and that NO x conversion is low under low load conditions because of the low catalyst temperatures and the NO/NO 2 ratio being higher than unity. It was also shown that NO x conversion exceeds 90 per cent when the catalyst temperature is higher than 530 K. To investigate the details of the chemistry and thermofluid dynamics within the urea-SCR system, a computational fluid dynamics (CFD) code that incorporates detailed surface chemistry was developed based on the modified subroutines of CHEMKIN-II. The spatial variations of chemical species including NO and NH 3 in a thin catalyst channel was calculated using the model. The calculated result of NO conversion showed relatively good agreement with experimental results.
    Type of Medium: Online Resource
    ISSN: 1468-0874 , 2041-3149
    URL: Article
    DOI: 10.1243/146808705X7310
    RVK:
    ZG 1100
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2005
    detail.hit.zdb_id: 2030603-9
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  • 7
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    Online Resource
    Origin of Primary Sensory Neurons Innervating the Buccal Stretch Receptor
    SAGE Publications ; 1999
    In:  Journal of Dental Research Vol. 78, No. 1 ( 1999-01), p. 49-53
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 78, No. 1 ( 1999-01), p. 49-53
    Abstract: The primary sensory neurons innervating mechanoreceptors in oro-facial regions have their cell bodies in either the trigeminal ganglion or the mesencephalic nucleus of the trigeminal nerve. The buccal stretch receptor (BSR), a type of mechanoreceptor in the jaw of rodents, has recently been recognized as signaling the position of the mandible. The location of the primary afferent neurons innervating this receptor is unknown. To investigate the cell bodies of the BSR afferent neurons in rats, we applied wheat germ agglutinin-horseradish peroxidase (WGA-HRP) to the proximal stump of the severed nerve branch of the buccal nerve that supplied the BSR. HRP-labeled cell bodies were observed in the posterolateral portion of the ipsilateral trigeminal ganglion. None was found in the contralateral trigeminal ganglion or in the brainstem. All labeled cell bodies were oval or round and closely resembled pseudo-unipolar neurons. The mean diameter of the labeled somata ranged between 25.5 and 52.5 μm, with small (≤ 30 μm), medium (from 31 to 40 μm), and large somata (≥ 41 μm) accounting for 8.8%, 54.9%, and 36.3%, respectively. Among the myelinated nerve fibers in the branch in which WGA-HRP was applied, 78.5% terminated in the BSR and had larger fiber diameters than the rest, indicating that most of the medium and large HRP-labeled cell bodies were BSR afferents. From these results and the ontogenetic origin of this receptor, it is suggested that the BSR differentiated from the mechanoreceptors in the oral mucosa or the fascia of masticatory muscles.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345990780010601
    Language: English
    Publisher: SAGE Publications
    Publication Date: 1999
    detail.hit.zdb_id: 2057074-0
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