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  • SAGE Publications  (45)
  • 1
    Online Resource
    Online Resource
    Mineralized polycaprolactone nanofibrous matrix for odontogenesis of human dental pulp cells
    SAGE Publications ; 2014
    In:  Journal of Biomaterials Applications Vol. 28, No. 7 ( 2014-03), p. 1069-1078
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 28, No. 7 ( 2014-03), p. 1069-1078
    Abstract: The aim of the present study was to fabricate mineralized polycaprolactone nanofibrous scaffold and investigate its ability to elicit odontogenic differentiation of human dental pulp cells, compared to the pure polycaprolactone scaffold. Polycaprolactone nanofibrous scaffold was produced by electrospinning, and the surface was mineralized with apatite. Cellular behaviors on the mineralized polycaprolactone scaffold were assessed in terms of cell adhesion, growth, and odontoblastic differentiation. To evaluate the signal transduction of human dental pulp cells, mRNA expression was analyzed and Western blotting was performed. Mineralized polycaprolactone showed improved cell proliferation, mineralized nodule formation, and expression of odontoblastic marker genes including alkaline phosphatase, osteopontin, osteocalcin, dentin sialophosphoprotein (DSPP), and dentin matrix protein-1, as compared with pure polycaprolactone. Although the cell adhesion on the mineralized polycaprolactone was similar to that of the polycaprolactone, the expression level of proteins including collagen type I and the key adhesion receptor (integrin components α1, α2, and β1) was upregulated in mineralized polycaprolactone compared to polycaprolactone. Especially, cells seeded onto mineralized polycaprolactone scaffolds showed significantly increased levels of phosphorylated focal adhesion kinase, a marker of integrin activation, and downstream pathways, such as phosphor (p)-Akt, p-extracellular signal regulated kinase, p-c Jun N-terminal kinase, nuclear factor-kappa B, c-fos, and c-jun, compared with pure polycaprolactone. The mineralized polycaprolactone scaffold is attractive for dentin tissue engineering by promoting growth and odontogenic differentiation of human dental pulp cells through the integrin-mediated signaling pathway.
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328213495903
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2072559-0
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  • 2
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    Odontogenic stimulation of human dental pulp cells with bioactive nanocomposite fiber
    SAGE Publications ; 2015
    In:  Journal of Biomaterials Applications Vol. 29, No. 6 ( 2015-01), p. 854-866
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 29, No. 6 ( 2015-01), p. 854-866
    Abstract: The aim of the present study was to investigate the effects of a composite nanofibrous matrix made of biopolymer blend polycaprolactone-gelatin (BP) and mesoporous bioactive glass nanoparticles (BGNs) on the odontogenic differentiation of human dental pulp cells (HDPCs). BGN-BP nanomatrices, with BGN content of up to 20 wt%, were produced via electrospinning. The differentiation of the HDPCs was evaluated by using an ALP activity assay, calcified nodule formation, and mRNA expression for markers. Integrin and its underlying signal pathways were assessed via reverse transcriptase-polymerase chain reaction and Western blot analysis. Although cell growth and attachment on the BGN-BP nanomatrix was similar to that on BP, ALP activity, mineralized nodule formation, and mRNA, expressions involving ALP, osteocalcin, osteopontin, dentin sialophosphoprotein, and dentin matrix protein-1 were greater on BGN-BP. BGN-BP upregulated the key adhesion receptors (integrin components α1, α2, α5, and β1) and activated integrin downstream pathways, such as phosphorylated-focal adhesion kinase (p-FAK), and p-paxillin. In addition, BGN-BP activated BMP receptors, BMP-2 mRNA, and p-Smad 1/5/8, and such activation was blocked by the BMP antagonist, noggin. Furthermore, BGN-BP induced phosphorylation of extracellular signal-regulated kinase, protein kinase 38, and c-Jun-N-terminal kinase mitogen-activated protein kinases and activated expression of the transcription factors Runx2 and Osterix in HDPCs. Collectively, the results indicated for the first time that a BGN-BP composite nanomatrix promoted odontogenic differentiation of HDPCs through the integrin, BMP, and mitogen-activated protein kinases signaling pathway. Moreover, the nanomatrix is considered to be promising scaffolds for the culture of HDPCs and dental tissue engineering.
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328214546884
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2072559-0
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  • 3
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    Effects of bioactive cements incorporating zinc-bioglass nanoparticles on odontogenic and angiogenic potential of human dental pulp cells
    SAGE Publications ; 2015
    In:  Journal of Biomaterials Applications Vol. 29, No. 7 ( 2015-02), p. 954-964
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 29, No. 7 ( 2015-02), p. 954-964
    Abstract: The objective of this study was to investigate the effects of bioactive calcium phosphate cements (CPC, α-tricalcium phosphate-based) incorporating zinc-bioglass (ZnBG) on the odontogenic differentiation and angiogenesis of human dental pulp cells (HDPCs). Methods BGs with varying concentrations of Zn (0, 2.5 and 5%) were produced via a sol-gel process. The proliferation of HDPCs on CPC/BGs was determined by MTS assay. Alizarin red staining, RT-PCR, and ALP activity were used to assess odontogenic differentiation, and western blot analysis was used to asses signaling pathways. In vitro angiogenesis was examined via mRNA expression of angiogenic genes and tubule formation. Results All cement formulations showed no cytotoxicity. The CPCs with ZnBG showed increased ALP activity, enhanced formation of mineralized nodules, and upregulated mRNA expression of DMP-1, DSPP, Runx2, and osterix in a time- and dose-dependent manner, relative to CPCs without Zn. ZnBG upregulated integrins α1, α2, β1, and β3 and activated integrin downstream signal pathways, such as p-FAK, p-Akt, p-paxillin, RhoA, MAPK, and NF-κB, as well as canonical and non-canonical Wnt signaling. In addition, ZnBG upregulated VEGF mRNA in HDPCs and increased the tubular structure in endothelial cells. Conclusions Our results demonstrate that ZnBG incorporated within CPCs activates odontogenic differentiation and promotes angiogenesis in vitro through integrin, Wnt, MAPK, and NF-κB pathways. Thus, CPCs incorporating ZnBG are promising matrices in tissue engineering to stimulate endodontic regeneration.
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328214550896
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2072559-0
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  • 4
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    Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia
    SAGE Publications ; 2015
    In:  Cell Transplantation Vol. 24, No. 2 ( 2015-02), p. 183-190
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 24, No. 2 ( 2015-02), p. 183-190
    Abstract: The long-term effect of magnetically targeted neural stem cells in a rat focal cerebral ischemia model was investigated. In middle cerebral artery occlusion (MCAO) stroke model rats, ferumoxide-labeled human neural stem cells (hNSCs) were injected into the tail vein. MCAO rats were divided into three groups: ischemia only (IO), ischemia with NSC injection (IC), and ischemia with NSC injection and the use of magnet targeting (IM). Four weeks after MCAO and 3 weeks posttransplantation, a greater number of hNSCs were found in ischemic lesion sites in IM rat brain compared with IO and IC animals. In addition, differentiation of hNSCs into neurons or astrocytes and angiogenesis were markedly increased. In IM rats, infarct volume was considerably reduced, and function was significantly improved. The present study indicates that long-term use of magnetic fields may be a useful way to improve the efficacy of targeted migration of stem cells and functional deficits in stem cell-based therapy for ischemic brain injury.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368913X675755
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2020466-8
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  • 5
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    Depressive symptoms of house-poor persons: Korean panel data evidence
    SAGE Publications ; 2016
    In:  International Journal of Social Psychiatry Vol. 62, No. 6 ( 2016-09), p. 569-577
    Details
    In: International Journal of Social Psychiatry, SAGE Publications, Vol. 62, No. 6 ( 2016-09), p. 569-577
    Abstract: There are no studies researching the relationship between house-poor persons and mental health. Therefore, this study aimed to investigate the relationship between house-poor status and depressive symptoms. Aim: To examine the relationship between the house-poor and depressive symptoms according to household income. Methods: Data from the Korean Welfare Panel Study were used. House-poor were defined as people having possession with over 10% house-related interest in disposable income. About 7,565 participants over the age of 19 years were followed up from 2011 to 2013. The generalized estimating equations were used for analysis. Results: Individuals with more house-related debt showed increasingly higher depression scores (possession with under 5% related debt to disposable income β = 0.2024, p = .1544; under 10% β = 0.7030, p = .0008; over 10% β = 1.3207, p  〈  .0001). Individuals possessing houses with over 10% ratio of house-related debts to disposable income had higher depression scores than individuals without house ownership (no possession β = 0.8927, p  〈  .0001). Conclusion: Individuals without houses and individuals owning houses with higher percentages of house-related interests showed higher levels of depressive symptoms. Therefore, this study affirmed that the importance of considering the most vulnerable groups in addressing the mental health of individual.
    Type of Medium: Online Resource
    ISSN: 0020-7640 , 1741-2854
    URL: Article
    DOI: 10.1177/0020764016653773
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2066492-8
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  • 6
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    Circulating Endothelial Progenitor Cells as a Pathogenetic Marker of Moyamoya Disease
    SAGE Publications ; 2008
    In:  Journal of Cerebral Blood Flow & Metabolism Vol. 28, No. 11 ( 2008-11), p. 1795-1803
    Details
    In: Journal of Cerebral Blood Flow & Metabolism, SAGE Publications, Vol. 28, No. 11 ( 2008-11), p. 1795-1803
    Abstract: Moyamoya disease (MMD) is an unusual form of chronic cerebrovascular occlusive disease that involves the formation of characteristically abnormal vessels. Recent studies have reported that colony-forming unit (CFU) and outgrowth cells represent a subpopulation of endothelial progenitor cells (EPCs). Here, we attempted to determine the significance of CFU number and outgrowth cell yield in MMD. Endothelial progenitor cells were isolated from the blood of 24 adult MMD patients and from 48 age- and risk factor-matched control subjects. After 7 days of culture, CFUs were determined, and yields of outgrowth cells were measured during 2 months of culture. The EPC function was also evaluated using matrigel plate assays. It was found that CFU numbers were significantly lower in MMD patients than in controls. Moreover, during long-term culture, outgrowth cells were isolated from only 10% of control subjects but from 33% of MMD patients, and CFU numbers and tube formation were found to be lower in advanced MMD cases than in those with early stage disease, whereas outgrowth cells were more frequently detected in those with early MMD and moyamoya vessels than in those with advanced disease. These characteristics of circulating EPCs reflect mixed conditions of vascular occlusion and abnormal vasculogenesis during the pathogenesis of MMD.
    Type of Medium: Online Resource
    ISSN: 0271-678X , 1559-7016
    URL: Article
    DOI: 10.1038/jcbfm.2008.67
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2008
    detail.hit.zdb_id: 2039456-1
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  • 7
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    The Relationship Between Changes in Employment Status and Mortality Risk Based on the Korea Labor and Income Panel Study (2003-2008)
    SAGE Publications ; 2015
    In:  Asia Pacific Journal of Public Health Vol. 27, No. 2 ( 2015-03), p. NP993-NP1001
    Details
    In: Asia Pacific Journal of Public Health, SAGE Publications, Vol. 27, No. 2 ( 2015-03), p. NP993-NP1001
    Abstract: The aim of this study was to analyze the relationship between the mortality rate and changes in employment status. This study used mortality data from the Korean Labor and Income Panel Study. To analyze the relationship between the mortality rate and changes in employment status, the population was classified into employed, unemployed, or economically inactive. Demographic and socioeconomic variables such as gender, age, educational level, annual household income, marital status, and self-rated health status were controlled. In this study, the generalized estimating equations were used to analyze the relationship between the morality rate and the changes in employment status. The mortality rate was higher (odds ratio = 4.31) among the population that experienced a change in economic status from employed to unemployed than those who maintained employment. The mortality rate for the population who became unemployed or economically inactive was higher (odds ratio = 5.05) in cases of death by disease.
    Type of Medium: Online Resource
    ISSN: 1010-5395 , 1941-2479
    URL: Article
    DOI: 10.1177/1010539513486923
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2394975-2
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  • 8
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    Factors Influencing Unmet Need for Health Care Services in Korea
    SAGE Publications ; 2015
    In:  Asia Pacific Journal of Public Health Vol. 27, No. 2 ( 2015-03), p. NP2555-NP2569
    Details
    In: Asia Pacific Journal of Public Health, SAGE Publications, Vol. 27, No. 2 ( 2015-03), p. NP2555-NP2569
    Abstract: This study was conducted to investigate the prevalence of unmet need based on both subjective assessment and adjustments for need-predicted utilization and to examine factors associated with them. Data from the Fourth Korea National Health and Nutrition Examination Survey, a population-based, cross-sectional study, were used in this study. Participants included 11 620 Koreans (4959 males and 6661 females) aged 19 and older. The results for subjective unmet need suggested that female or younger individuals were more likely to experience unmet need, compared to male or older individuals. Those residing outside the capital had a higher likelihood of experiencing unmet need. Lower levels of education showed an association with higher odds of having unmet need. This study found similar results for the adjusted unmet need. The findings of this study suggest that location and education are more important factors associated with unmet need than income levels.
    Type of Medium: Online Resource
    ISSN: 1010-5395 , 1941-2479
    URL: Article
    DOI: 10.1177/1010539513490789
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2394975-2
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  • 9
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    Does hospital need more hospice beds? Hospital charges and length of stays by lung cancer inpatients at their end of life: A retrospective cohort design of 2002–2012
    SAGE Publications ; 2015
    In:  Palliative Medicine Vol. 29, No. 9 ( 2015-10), p. 808-816
    Details
    In: Palliative Medicine, SAGE Publications, Vol. 29, No. 9 ( 2015-10), p. 808-816
    Abstract: Previous studies found that hospice and palliative care reduces healthcare costs for end-of-life cancer patients. Aim: To investigate hospital inpatient charges and length-of-stay differences by availability of hospice care beds within hospitals using nationwide data from end-of-life inpatients with lung cancer. Design: A retrospective cohort study was performed using nationwide lung cancer health insurance claims from 2002 to 2012 in Korea. Setting and participants: Descriptive and multi-level (patient-level and hospital-level) mixed models were used to compare inpatient charges and lengths of stay. Using 673,122 inpatient health insurance claims, we obtained aggregated hospital inpatient charges and lengths of stay from a total of 114,828 inpatients and 866 hospital records. Results: Hospital inpatient charges and length of stay drastically increased as patients approached death; a significant portion of hospital inpatient charges and lengths of stay occurred during the end-of-life period. According to our multi-level analysis, hospitals with hospice care beds tend to have significantly lower end-of-life hospital inpatient charges; however, length of stay did not differ. Hospitals with more hospice care beds were associated with reduction in hospital inpatient charges within 3 months before death. Conclusion: Higher end-of-life healthcare hospital charges were found for lung cancer inpatients who were admitted to hospitals without hospice care beds. This study suggests that health policy-makers and the National Health Insurance program need to consider expanding the use of hospice care beds within hospitals and hospice care facilities for end-of-life patients with lung cancer in South Korea, where very limited numbers of resources are currently available.
    Type of Medium: Online Resource
    ISSN: 0269-2163 , 1477-030X
    URL: Article
    DOI: 10.1177/0269216315582123
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2027566-3
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  • 10
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    PPAR-δ agonist with mesenchymal stem cells induces type II collagen-producing chondrocytes in human arthritic synovial fluid
    SAGE Publications ; 2017
    In:  Cell Transplantation
    Details
    In: Cell Transplantation, SAGE Publications
    Type of Medium: Online Resource
    ISSN: 0963-6897
    URL: Article
    DOI: 10.3727/096368916X695218
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2020466-8
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