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  • 1
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    Online Resource
    Therapeutic Superiority for Cartilage Repair by CD271-Positive Marrow Stromal Cell Transplantation
    SAGE Publications ; 2013
    In:  Cell Transplantation Vol. 22, No. 7 ( 2013-07), p. 1201-1211
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 22, No. 7 ( 2013-07), p. 1201-1211
    Abstract: Recent reports indicated that human isolated CD271 + bone marrow mesenchymal stromal cells (BM-MSCs) have a greater expansion and potential for multipotent differentiation including chondrogenesis than classical plastic adherent (PA) BM-MSCs in vitro. Therefore, we set up a hypothesis that CD271 + MSCs may have a greater chondrogenic potential than PA-MSCs in vitro and in vivo. We investigated the superiority of CD271 + MSCs on chondrogenesis using in vitro expansion and pellet culture system and in vivo rat model of cartilage defect when compared to PA-MSCs. In the in vitro study, CD271 + MSCs showed higher expansion potential and produced larger pellets with higher expressions of chondrogenic genes when compared to the control groups. During the culture, CD271 expression decreased, which resulted in decreased chondrogenesis. In the in vivo study, immunohistochemical staining demonstrated differentiated human chondrocytes identified as double-stained cells with human-specific collagen type 2 and human leukocyte antigen-ABC in CD271 + and PA groups. The number of double-stained cells was significantly higher in the CD271 + group than PA group. Real-time RT-PCR analysis of tissue RNA isolated from the chondral defect site for human-specific chondrogenic markers demonstrated a significantly higher expression in CD271 + group than PA group. Macroscopic examination of chondral defect sites at week 8 revealed glossy white and well-integrated repaired tissues in the CD271 + and PA groups, but not in the PBS group. The average histological score in the CD271 + group was significantly greater than in the other groups. Apoptosis analysis at the cell transplanted site with TUNEL staining showed that the CD271 + group had significantly fewer apoptotic chondrocytes compared with the PA group. These results indicate that CD271 + MSCs have a greater chondrogenic potential than PA-MSCs in both in vitro and in vivo conditions.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368912X657378
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
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  • 2
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    Local Transplantation of Ex Vivo Expanded Bone Marrow-Derived CD34-Positive Cells Accelerates Fracture Healing
    SAGE Publications ; 2012
    In:  Cell Transplantation Vol. 21, No. 12 ( 2012-12), p. 2689-2709
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 21, No. 12 ( 2012-12), p. 2689-2709
    Abstract: Transplantation of bone marrow (BM) CD34 + cells, an endothelial/hematopoietic progenitor-enriched cell population, has shown therapeutic efficiency in the treatment of ischemic diseases enhancing neovascularization. However, the number of CD34 + cells obtained from bone marrow is not sufficient for routine clinical application. To overcome this issue, we developed a more efficient and clinically applicable CD34 + cell expansion method. Seven-day ex vivo expansion culture of BM CD34 + cells with a cocktail of five growth factors containing VEGF, SCF, IL-6, Flt-3 ligand, and TPO resulted in reproducible more than 20-fold increase in cell number. The favorable effect of the local transplantation of culture expanded (cEx)-BM CD34 + cells on rat unhealing fractures was equivalent or higher than that of nonexpanded (fresh) BM CD34 + cells exhibiting sufficient therapeutic outcome with frequent vasculogenic/osteogenic differentiation of transplanted cEx-BM CD34 + cells and fresh BM CD34 + cells as well as intrinsic enhancement of angiogenesis/osteogenesis at the treated fracture sites. Specifically, cEx-BM CD34 + cell treatment demonstrated the best blood flow recovery at fracture sites compared with the nonexpanded BM CD34 + cells. In vitro, cEx-BM CD34 + cells showed higher colony/tube-forming capacity than nonexpanded BM CD34 + cells. Both cells demonstrated differentiation potential into osteoblasts. Since fresh BM CD34 + cells can be easily collected from fracture sites at the time of primary operation and stored for future use, autologous cEx-BM CD34 + cell transplantation would be not only a simple but also a promising therapeutic strategy for unhealing fractures in the field of orthopedic trauma surgery.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368912X654920
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2012
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  • 3
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    Local Transplantation of G-CSF-Mobilized CD34 + Cells in a Patient with Tibial Nonunion: A Case Report
    SAGE Publications ; 2011
    In:  Cell Transplantation Vol. 20, No. 9 ( 2011-10), p. 1491-1496
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 20, No. 9 ( 2011-10), p. 1491-1496
    Abstract: Although implantation of crude bone marrow cells has been applied in a small number of patients for fracture healing, transplantation of peripheral blood CD34 + cells, the hematopoietic/endothelial progenitor cell-enriched population, in patients with fracture has never been reported. Here, we report the first case of tibial nonunion receiving autologous, granulocyte colony stimulating factor mobilized CD34 + cells accompanied with autologous bone grafting. No serious adverse event occurred, and the novel therapy performed 9 months after the primary operation resulted in bone union 3 months later without any symptoms including pain and gait disturbance.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368910X550189
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
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  • 4
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    Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion following Bone Fracture
    SAGE Publications ; 2015
    In:  Cell Transplantation Vol. 24, No. 7 ( 2015-07), p. 1379-1393
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 24, No. 7 ( 2015-07), p. 1379-1393
    Abstract: We recently demonstrated that the local transplantation of human peripheral blood (PB) CD34 + cells, an endothelial/hematopoietic progenitor cell-rich population, contributes to fracture repair via vasculogenesis/angiogenesis and osteogenesis. Human PB mononuclear cells (MNCs) are also considered a potential cell fraction for neovascularization. We have previously shown the feasibility of human PB MNCs to enhance fracture healing. However, there is no report directly comparing the efficacy for fracture repair between CD34 + cells and MNCs. In addition, an unhealing fracture model, which does not accurately resemble a clinical setting, was used in our previous studies. To overcome these issues, we compared the capacity of human granulocyte colony-stimulating factor-mobilized PB (GM-PB) CD34 + cells and human GM-PB MNCs in a nonunion model, which more closely resembles a clinical setting. First, the effect of local transplantation of 1 × 10 5 GM-PB CD34 + cells (CD34 + group), 1 × 10 7 GM-PB MNCs (containing approximately 1 × 10 5 GM-PB CD34 + cells) (MNC group), and phosphate-buffered saline (PBS) (PBS group) on nonunion healing was compared. Similar augmentation of blood flow recovery at perinonunion sites was observed in the CD34 + and MNC groups. Meanwhile, a superior effect on nonunion repair was revealed by radiological, histological, and functional assessment in the CD34 + group compared with the other groups. Moreover, through in vivo and in vitro experiments, excessive inflammation induced by GM-PB MNCs was confirmed and believed to be one of the mechanisms underlying this potency difference. These results strongly suggest that local transplantation of GM-PB CD34 + cells is a practical and effective strategy for treatment of nonunion after fracture.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368914X681586
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
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  • 5
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    Local Transplantation of Granulocyte Colony-Stimulating Factor-Mobilized Human Peripheral Blood Mononuclear Cells for Unhealing Bone Fractures
    SAGE Publications ; 2012
    In:  Cell Transplantation Vol. 21, No. 4 ( 2012-04), p. 707-721
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 21, No. 4 ( 2012-04), p. 707-721
    Abstract: We previously reported the therapeutic potential of human peripheral blood (hPB) CD34 + cells for bone fracture healing via vasculogenesis/angiogenesis and osteogenesis. Transplantation of not only hPB CD34 + cells but also hPB total mononuclear cells (MNCs) has shown their therapeutic efficiency for enhancing ischemic neovascularization. Compared with transplantation of purified hPB CD34 + cells, transplantation of hPB MNCs is more attractive due to its simple method of cell isolation and inexpensive cost performance in the clinical setting. Thus, in this report, we attempted to test a hypothesis that granulocyte colony-stimulating factor-mobilized (GM) hPB MNC transplantation could also contribute to fracture healing via vasculogenesis/angiogenesis and osteogenesis. Nude rats with unhealing fractures received local administration of the following materials with atelocollagen: 1 × 10 7 GM hPB MNCs (Hi group), 1 × 10 6 GM hPB MNCs (Lo group), or PBS (PBS group). Immunohistochemistry and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated human cell-derived vasculogenesis and osteogenesis in the Hi and Lo groups, but not in the PBS group at week 1. Intrinsic angiogenesis and osteogenesis assessed by rat capillary, osteoblast density, and real-time RT-PCR analysis was significantly enhanced in the Hi group compared to the other groups. Blood flow assessment by laser doppler perfusion imaging showed a significantly higher blood flow ratio at week 1 in the Hi group compared with the other groups. Morphological fracture healing was radiographically and histologically confirmed in about 30% of animals in the Hi group at week 8, whereas all animals in the other groups resulted in nonunion. Local transplantation of GM hPB MNCs contributes to fracture healing via vasculogenesis/angiogenesis and osteogenesis.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.3727/096368911X582769a
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2012
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  • 6
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    Choroid plexus AVM with anomalous origin of the capsulothalamic artery: A case report
    SAGE Publications ; 2018
    In:  Interventional Neuroradiology Vol. 24, No. 1 ( 2018-02), p. 76-81
    Details
    In: Interventional Neuroradiology, SAGE Publications, Vol. 24, No. 1 ( 2018-02), p. 76-81
    Abstract: Traditionally, it has been believed that the plexal segment of the anterior choroidal artery (AChoA) can be sacrificed safely. Here, we present a case of choroid plexus arteriovenous malformation (AVM) in which the capsulothalamic artery originated from distal plexal segment of the AChoA. Clinical presentation A 45-year-old man was diagnosed with arteriovenous malformation involving the left inferior horn in screening MRI. Preceding stereotactic radiosurgery, transarterial target embolization was performed. In this procedure, 20% n-butyl-2-cyanoacrylate (NBCA) was successfully injected from the lateral plexal branch of the AChoA. After embolization, right homonymous hemianopsia developed due to cerebral infarction on the left optic radiation. This infarction was considered to be within the territory of the capsulothalamic artery. Conclusion This anomalous capsulothalamic artery might be formed by hemodynamic compromise of the brain surrounding AVM in early gestation. We must be aware of this unusual anatomical variation to avoid ischemic complication in embolization of the AChoA.
    Type of Medium: Online Resource
    ISSN: 1591-0199 , 2385-2011
    URL: Article
    DOI: 10.1177/1591019917739841
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2018
    detail.hit.zdb_id: 2571161-1
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  • 7
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    Comparison of simultaneous versus staged bilateral total hip arthroplasty via the direct anterior approach: A propensity score matched analysis
    SAGE Publications ; 2023
    In:  Journal of Orthopaedic Surgery Vol. 31, No. 2 ( 2023-05)
    Details
    In: Journal of Orthopaedic Surgery, SAGE Publications, Vol. 31, No. 2 ( 2023-05)
    Abstract: It remains unclear whether simultaneous bilateral total hip arthroplasty (SimBTHA) or staged bilateral total hip arthroplasty (StaBTHA) is clinically superior. No study has compared these two procedures matching surgical approach and patient background. This study aimed to clarify the differences between SimBTHA using direct anterior approach (SimBTHA-DAA) and StaBTHA using the direct anterior approach (StaBTHA-DAA). Methods Patients who underwent THA between 2012 and 2020 were enrolled, resulting in a total of 1658 hips of 1388 patients. After propensity score matching for patient background, 204 hips of 102 patients (51 patients in each group) were examined. Clinical and radiographic outcomes, complications, intraoperative blood loss and blood transfusions (BT) were evaluated. In complications, we evaluated periprosthetic fractures, pulmonary embolism, deep venous thrombosis, surgical site infection and dislocation. Results At the final follow-up, clinical and radiographic outcomes and complications were not significantly different between the groups. Intraoperative blood loss was equivalent for SimBTHA and the sum in the first- and second-stage StaBTHA. The total-BT rate was significantly higher for SimBTHA-DAA than for StaBTHA-DAA ( p 〈 .0001). The allogeneic BT rate was significantly higher in SimBTHA-DAA in the supine position (32.3%) than in StaBTHA-DAA (8.3%) ( p = .007). However, no patient who received autologous BT required allogeneic BT. Conclusions Clinical and radiographic outcomes were equivalent between SimBTHA-DAA and StaBTHA-DAA. The allogeneic BT rate was significantly higher in SimBTHA-DAA than in StaBTHA-DAA. Autologous BT reduced the use of allogeneic BT in SimBTHA-DAA. Auto-BT may be useful for avoiding allo-BT in SimBTHA.
    Type of Medium: Online Resource
    ISSN: 1022-5536 , 2309-4990
    URL: Article
    DOI: 10.1177/10225536231180328
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2128854-9
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  • 8
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    Potential Interaction Between Sepsis and Acute Respiratory Distress Syndrome and Effect on the 6-Month Clinical Outcomes: A Preliminary Secondary Analysis of a Prospective Observational Study
    SAGE Publications ; 2023
    In:  Journal of Intensive Care Medicine Vol. 38, No. 1 ( 2023-01), p. 60-69
    Details
    In: Journal of Intensive Care Medicine, SAGE Publications, Vol. 38, No. 1 ( 2023-01), p. 60-69
    Abstract: This study aimed to investigate the effect of the potential interaction between sepsis and acute respiratory distress syndrome (ARDS) on the 6-month clinical outcomes. Methods This secondary analysis of a prospective multicenter observational study included patients who were expected to receive mechanical ventilation for more than 48 h. Patients were stratified based on the incidence of sepsis and further subdivided according to the presence of ARDS. The primary endpoints for patients whose follow-up information was available included mortality (n = 162) and the occurrence of PICS (n = 96) at six months. The diagnosis of PICS was based on any of the following criteria: (1) decrease ≥ 10 points in the physical component score of the 36-item Short Form (SF36) questionnaire; (2) decrease ≥ 10 points in the mental component score of the SF-36; or (3) decline in the Short Memory Questionnaire (SMQ) score and SMQ score 〈 40 at six months after ICU admission. We conducted multivariate logistic regression analyses to assess the effect of the potential interaction between ARDS and sepsis on the 6-month clinical outcomes. Results The mortality in the ARDS sub-group was higher than that in the non-ARDS subgroup [47% (7/15) versus 21% (18/85)] in the non-sepsis group. However, the mortality in the ARDS and non-ARDS subgroups was similar in the sepsis group. Multivariate logistic regression analyses revealed that ARDS was significantly associated with mortality in the non-sepsis group (adjusted OR: 5.25; 95% CI: 1.45-19.09; p = .012), but not in the sepsis group (P for interaction = .087). Multivariate logistic regression analyses showed ARDS was not associated with PICS occurrence in the non-sepsis and sepsis groups (P-value for the interaction = .039). Conclusions This hypothesis-generating study suggested that the effect of ARDS on the 6-month outcomes depended on the presence or absence of sepsis. Trial registration Not applicable
    Type of Medium: Online Resource
    ISSN: 0885-0666 , 1525-1489
    URL: Article
    DOI: 10.1177/08850666221107559
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2001472-7
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