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  • SAGE Publications  (9)
  • 1
    Online Resource
    Online Resource
    Molecular Cloning of the Genes Encoding the PR55/Bβ/δ Regulatory Subunits for PP-2A and Analysis of Their Functions in Regulating Development of Goldfish, Carassius auratus
    SAGE Publications ; 2010
    In:  Gene Regulation and Systems Biology Vol. 4 ( 2010-01), p. GRSB.S6065-
    Details
    In: Gene Regulation and Systems Biology, SAGE Publications, Vol. 4 ( 2010-01), p. GRSB.S6065-
    Abstract: The protein phosphatase-2A (PP-2A), one of the major phosphatases in eukaryotes, is a heterotrimer, consisting of a scaffold A subunit, a catalytic C subunit and a regulatory B subunit. Previous studies have shown that besides regulating specific PP-2A activity, various B subunits encoded by more than 16 different genes, may have other functions. To explore the possible roles of the regulatory subunits of PP-2A in vertebrate development, we have cloned the PR55/B family regulatory subunits: β and δ, analyzed their tissue specific and developmental expression patterns in Goldfish ( Carassius auratus). Our results revealed that the full-length cDNA for PR55/Bβ consists of 1940 bp with an open reading frame of 1332 nucleotides coding for a deduced protein of 443 amino acids. The full length PR55/Bδ cDNA is 2163 bp containing an open reading frame of 1347 nucleotides encoding a deduced protein of 448 amino acids. The two isoforms of PR55/B display high levels of sequence identity with their counterparts in other species. The PR55/Bβ mRNA and protein are detected in brain and heart. In contrast, the PR55/Bδ is expressed in all 9 tissues examined at both mRNA and protein levels. During development of goldfish, the mRNAs for PR55/Bβ and PR55/Bδ show distinct patterns. At the protein level, PR55/Bδ is expressed at all developmental stages examined, suggesting its important role in regulating goldfish development. Expression of the PR55/Bδ anti-sense RNA leads to significant downregulation of PR55/Bδ proteins and caused severe abnormality in goldfish trunk and eye development. Together, our results suggested that PR55/Bδ plays an important role in governing normal trunk and eye formation during goldfish development.
    Type of Medium: Online Resource
    ISSN: 1177-6250 , 1177-6250
    URL: Article
    DOI: 10.4137/GRSB.S6065
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2010
    detail.hit.zdb_id: 2383407-9
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  • 2
    Online Resource
    Online Resource
    The Goldfish SG2NA Gene Encodes Two α-Type Regulatory Subunits for PP-2A and Displays Distinct Developmental Expression Pattern
    SAGE Publications ; 2009
    In:  Gene Regulation and Systems Biology Vol. 3 ( 2009-01), p. GRSB.S2764-
    Details
    In: Gene Regulation and Systems Biology, SAGE Publications, Vol. 3 ( 2009-01), p. GRSB.S2764-
    Abstract: SG2NA is a member of the striatin protein family. In human and mouse, the SG2NA gene encodes two major protein isoforms: SG2NAα and SG2NAβ. The functions of these proteins, except for acting as the regulatory subunits for PP-2A, remain largely unknown. To explore the possible functions of SG2NA in lower vertebrates, we have isolated two SG2NA cDNAs from goldfish, Carassius auratus. Our results reveal that the first cDNA contains an ORF of 2118 bp encoding a deduced protein with 705 amino acids, and the second one 2148 bp coding for a deduced protein of 715 amino acids. Comparative analysis reveals that both isoforms belong to the α-type, and are named SG2NAα and SG2NAα + . RT-PCR and western blot analysis reveal that the SG2NA gene is differentially expressed in 9 tissues examined. During goldfish development, while the SG2NA mRNAs remain relatively constant in the first 3 stages and then become decreased and fluctuated from gastrula to larval hatching, the SG2NA proteins are fluctuated, displaying a peak every 3 to 4 stages. Each later peak is higher than the earlier one and the protein expression level becomes maximal at hatching stage. Together, our results reveal that SG2NA may play an important role during goldfish development and also in homeostasis of most adult tissues.
    Type of Medium: Online Resource
    ISSN: 1177-6250 , 1177-6250
    URL: Article
    DOI: 10.4137/GRSB.S2764
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2009
    detail.hit.zdb_id: 2383407-9
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  • 3
    Online Resource
    Online Resource
    Probability Analysis of Corrosion Process of Corroded RC Flexural Member in Marine Environment
    SAGE Publications ; 2014
    In:  Advances in Structural Engineering Vol. 17, No. 9 ( 2014-09), p. 1299-1314
    Details
    In: Advances in Structural Engineering, SAGE Publications, Vol. 17, No. 9 ( 2014-09), p. 1299-1314
    Abstract: The main cause of deterioration in reinforced concrete (RC) structures is related to reinforcing steel corrosion, therefore, the calculation of steel corrosion loss should be evaluated in detail. The corrosion initiation time and concrete cracking time are random variables due to the various uncertainties in reinforcement corrosion process. Thus, RC structural member at a specific observation time of design period may be in three different states, namely no corrosion, ongoing corrosion but no cover concrete cracking, concrete cover cracking. Accordingly, a probabilistic model for corrosion loss of steel cross-section was developed in three critical phases. For illustrative purpose, statistic analysis of the percentage of corrosion was conducted using Monte Carlo simulation for a simply supported RC flexural member, in which the occurrence probabilities of above three different states at an observation time were determined. The statistical results showed that percentage of corrosion can be described by a double-peak probability distribution; the occurrence probabilities of no corrosion and no concrete cracking decrease with time; the reliability of RC flexural element in marine environment significantly decreases with time due to reinforcement corrosion.
    Type of Medium: Online Resource
    ISSN: 1369-4332 , 2048-4011
    URL: Article
    DOI: 10.1260/1369-4332.17.9.1299
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2026561-X
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  • 4
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    Online Resource
    Radial Extracorporeal Shock Wave Therapy as a Novel Agent for Benign Prostatic Hyperplasia Refractory to Current Medical Therapy
    SAGE Publications ; 2019
    In:  American Journal of Men's Health Vol. 13, No. 1 ( 2019-01), p. 155798831983189-
    Details
    In: American Journal of Men's Health, SAGE Publications, Vol. 13, No. 1 ( 2019-01), p. 155798831983189-
    Abstract: This study aimed to assess efficacy and safety data from pilot trials of the radial extracorporeal shock wave therapy (rESWT) to treat benign prostatic hyperplasia (BPH) refractory to current medical therapy. A total of 29 men with lower urinary tract symptoms (LUTS) suggestive of BPH who had responded poorly to medical therapy for at least 6 months and were poor surgical candidates were enrolled. Each participant was treated with rESWT once a week for 8 weeks, each by 2000 impulses at 2.0 bar and 10 hertz of frequency. International Prostate Symptom Score (IPSS), quality of life (QoL), and International Index of Erectile Function-5 (IIEF-5) were evaluated before treatment, after the fourth and eighth rESWT, and 3 months after the end of treatment. Peak urinary flow ( Q max ) and postvoid residual (PVR) were assessed. Safety was also documented. Statistically significant clinical improvements were reported for IPSS, QoL, and IIEF-5 after treatment, and those were sustained until 3 months follow-up. Q max and PVR improved evidently at 8 weeks with a 63% and 70% improvement, respectively. The only adverse event was the occasional perineum pain or discomfort, which usually disappeared within 3 days. The rESWT may be an effective, safe, and noninvasive treatment for symptomatic BPH in selected patients whose medical treatment has faced failure and are poor surgical candidates.
    Type of Medium: Online Resource
    ISSN: 1557-9883 , 1557-9891
    URL: Article
    DOI: 10.1177/1557988319831899
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2019
    detail.hit.zdb_id: 2275106-3
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  • 5
    Online Resource
    Online Resource
    Generating synthesized computed tomography from CBCT using a conditional generative adversarial network for head and neck cancer patients
    SAGE Publications ; 2022
    In:  Technology in Cancer Research & Treatment Vol. 21 ( 2022-01), p. 153303382210853-
    Details
    In: Technology in Cancer Research & Treatment, SAGE Publications, Vol. 21 ( 2022-01), p. 153303382210853-
    Abstract: Purpose: To overcome the imaging artifacts and Hounsfield unit inaccuracy limitations of cone-beam computed tomography, a conditional generative adversarial network is proposed to synthesize high-quality computed tomography-like images from cone-beam computed tomography images. Methods: A total of 120 paired cone-beam computed tomography and computed tomography scans of patients with head and neck cancer who were treated during January 2019 and December 2020 retrospectively collected; the scans of 90 patients were assembled into training and validation datasets, and the scans of 30 patients were used in testing datasets. The proposed method integrates a U-Net backbone architecture with residual blocks into a conditional generative adversarial network framework to learn a mapping from cone-beam computed tomography images to pair planning computed tomography images. The mean absolute error, root-mean-square error, structural similarity index, and peak signal-to-noise ratio were used to assess the performance of this method compared with U-Net and CycleGAN. Results: The synthesized computed tomography images produced by the conditional generative adversarial network were visually similar to planning computed tomography images. The mean absolute error, root-mean-square error, structural similarity index, and peak signal-to-noise ratio calculated from test images generated by conditional generative adversarial network were all significantly different than CycleGAN and U-Net. The mean absolute error, root-mean-square error, structural similarity index, and peak signal-to-noise ratio values between the synthesized computed tomography and the reference computed tomography were 16.75 ± 11.07 Hounsfield unit, 58.15 ± 28.64 Hounsfield unit, 0.92 ± 0.04, and 30.58 ± 3.86 dB in conditional generative adversarial network, 20.66 ± 12.15 Hounsfield unit, 66.53 ± 29.73 Hounsfield unit, 0.90 ± 0.05, and 29.29 ± 3.49 dB in CycleGAN, and 16.82 ± 10.99 Hounsfield unit, 58.68 ± 28.34 Hounsfield unit, 0.92 ± 0.04, and 30.48 ± 3.83 dB in U-Net, respectively. Conclusions: The synthesized computed tomography generated from the cone-beam computed tomography-based conditional generative adversarial network method has accurate computed tomography numbers while keeping the same anatomical structure as cone-beam computed tomography. It can be used effectively for quantitative applications in radiotherapy.
    Type of Medium: Online Resource
    ISSN: 1533-0346 , 1533-0338
    URL: Article
    DOI: 10.1177/15330338221085358
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2146365-7
    detail.hit.zdb_id: 2220436-2
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  • 6
    Online Resource
    Online Resource
    Association of susceptibility to multiple sclerosis in Southern Han Chinese with HLA-DRB1, -DPB1 alleles and DRB1-DPB1 haplotypes: distinct from other populations
    SAGE Publications ; 2009
    In:  Multiple Sclerosis Journal Vol. 15, No. 12 ( 2009-12), p. 1422-1430
    Details
    In: Multiple Sclerosis Journal, SAGE Publications, Vol. 15, No. 12 ( 2009-12), p. 1422-1430
    Abstract: Association of HLA class II with multiple sclerosis (MS) has been widely studied in both Western and Oriental populations. However, such an association is not well documented in Chinese. The objective of this study was to examine the association between the susceptibility to conventional MS in Southern Chinese with HLA-DRB1,-DPB1 alleles and putative DRB1-DPB1 haplotypes. Genotyping of HLA-DRB1 and -DPB1 alleles was performed in 60 patients with conventional MS and 95 controls. Allele frequencies were compared between patients and controls to identify MSassociated alleles. Relative predisposing effect method was used to compare haplotype frequencies in patients and controls and to identify possible predisposing DRB1-DPB1 haplotypes, which were further examined for differences in haplotype carriage rates between the two groups. We found that the allele frequency of DRB1*1501 was not different between patients (18.3%) and controls (21.1%) ( p = 0.837). In contrast, frequency of the DPB1*0501 allele was significantly higher in patients (90%) than in controls (67.4%) (odds ratio = 4.36, p = 0.0013, pcorr = 0.025). DRB1-DPB1 linkage haplotype in patients (8.33%) was significantly higher than in controls (0%) ( p 〈 0.0001) and the carriage rate of this haplotype was significantly increased in patients (15%) as compared with controls (0%) ( p = 0.00013, pcorr = 0.003). Combined, these results suggest that HLA-DRB1*1501 is not associated with susceptibility to conventional MS in Southern Chinese. Instead, both the DPB1*0501 allele and the DRB1*1602- DPB1*0501 haplotype are strong predisposing factors for conventional MS in this population. Our results establish that the HLA profiles of MS in Southern Chinese are distinct from other populations.
    Type of Medium: Online Resource
    ISSN: 1352-4585 , 1477-0970
    URL: Article
    DOI: 10.1177/1352458509345905
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2009
    detail.hit.zdb_id: 2008225-3
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  • 7
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    Applications of hypervalent iodine(III) reagents in constructing ortho -iodo aromatic ethers
    SAGE Publications ; 2021
    In:  Journal of Chemical Research Vol. 45, No. 9-10 ( 2021-09), p. 818-822
    Details
    In: Journal of Chemical Research, SAGE Publications, Vol. 45, No. 9-10 ( 2021-09), p. 818-822
    Abstract: A one-pot method for the synthesis of aromatic ethers using hypervalent iodine(III) reagents obtained from the corresponding iodoaryl compounds is developed. In this concise method, six diaryl ethers and three heterocyclic aromatic ethers are synthesized in good yields. Furthermore, possible mechanisms for the syntheses of the hypervalent iodine reagents and construction of the aromatic ethers are proposed.
    Type of Medium: Online Resource
    ISSN: 1747-5198 , 2047-6507
    URL: Article
    DOI: 10.1177/17475198211005385
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 3010810-X
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  • 8
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    Online Resource
    Genotype profiles of Helicobacter pylori from gastric biopsies and strains with antimicrobial-induced resistance
    SAGE Publications ; 2020
    In:  Therapeutic Advances in Gastroenterology Vol. 13 ( 2020-01), p. 175628482095259-
    Details
    In: Therapeutic Advances in Gastroenterology, SAGE Publications, Vol. 13 ( 2020-01), p. 175628482095259-
    Abstract: The genotypic method could significantly shorten the time needed to obtain antibiotic susceptibility data for Helicobacter pylori. The aim of this study was to explore the profile of H. pylori from gastric biopsies and strains with antibiotic-induced resistance. Methods: A total of 124 gastric biopsies were used to perform gene sequencing and to perform bacterial culture and susceptibility testing. Seven susceptible strains were selected to develop resistance to clarithromycin, levofloxacin, and metronidazole. Four susceptible strains were selected to transfer candidate mutations. The genotype profiles of these groups were analyzed by sequencing analysis. The antibiotic susceptibility of these strains was detected using the E-test method. Results: Phenotypic resistance to clarithromycin, levofloxacin, and metronidazole was observed in 35.5%, 40.0%, and 79.8% strains, respectively. Point mutations in 23 S rRNA, gyrA, and rdxA genes were observed in 39.5%, 38.7%, and 86.3% of gastric biopsies, respectively. The A2143G mutation in the 23S rRNA occurs in most clarithromycin-resistant samples. The A2142C point mutation showed a higher efficacy than A2142G and A2143G for inducing clarithromycin resistance. The D91N and N87K mutations in gyrA occurs in most levofloxacin-resistant samples, and double point mutations showed a higher efficacy than single mutations for inducing levofloxacin resistance. Phenotypic resistance and mutations in rdxA lacked consistency. Conclusion: Genotype-based gastric biopsy analysis was reliable for determining clarithromycin and levofloxacin resistance. A2143G in 23S rRNA and N87K/D91N in the gyrA gene occurred in most resistant strains. Mutations in the rdxA gene were not good indicators of metronidazole resistance.
    Type of Medium: Online Resource
    ISSN: 1756-2848 , 1756-2848
    URL: Article
    DOI: 10.1177/1756284820952596
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2440710-0
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  • 9
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    Factors associated with failure of high-flow nasal cannula oxygen therapy in patients with severe COVID-19: a retrospective case series
    SAGE Publications ; 2022
    In:  Journal of International Medical Research Vol. 50, No. 5 ( 2022-05), p. 030006052211035-
    Details
    In: Journal of International Medical Research, SAGE Publications, Vol. 50, No. 5 ( 2022-05), p. 030006052211035-
    Abstract: To identify factors associated with high-flow nasal cannula (HFNC) therapy failure in patients with severe COVID-19. Methods We retrospectively examined clinical and laboratory data upon admission, treatments, and outcomes of patients with severe COVID-19. Sequential Organ Failure Assessment (SOFA) scores were also calculated. Results Of 54 patients with severe COVID-19, HFNC therapy was successful in 28 (51.9%) and unsuccessful in 26 (48.1%). HFNC therapy failure was more common in patients aged ≥60 years and in men. Compared with patients with successful HFNC therapy, patients with HFNC therapy failure had higher percentages of fatigue, anorexia, and cardiovascular disease; a longer time from symptom onset to diagnosis; higher SOFA scores; a higher body temperature, respiratory rate, and heart rate; more complications, including acute respiratory distress syndrome, septic shock, myocardial damage, and acute kidney injury; a higher C-reactive protein concentration, neutrophil count, and prothrombin time; and a lower arterial partial pressure of oxygen/fraction of inspired oxygen (PaO 2 /FiO 2 ). However, male sex, a low PaO 2 /FiO 2 , and a high SOFA score were the only independent factors significantly associated with HFNC therapy failure. Conclusions Male sex, a low PaO 2 /FiO 2 , and a high SOFA score were independently associated with HFNC therapy failure in patients with severe COVID-19.
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    URL: Article
    DOI: 10.1177/03000605221103525
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2082422-1
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