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  • 1
    Online Resource
    Online Resource
    The novel potential biomarkers for multidrug-resistance tuberculosis using UPLC-Q-TOF-MS
    SAGE Publications ; 2020
    In:  Experimental Biology and Medicine Vol. 245, No. 6 ( 2020-03), p. 501-511
    Details
    In: Experimental Biology and Medicine, SAGE Publications, Vol. 245, No. 6 ( 2020-03), p. 501-511
    Abstract: The lack of rapid and efficient diagnostics impedes largely the epidemic control of multidrug-resistant tuberculosis, and might misguide the therapeutic strategies as well. This study aimed to identify novel multidrug-resistant tuberculosis biomarkers to improve the early intervention, symptomatic treatment and control of the prevalence of multidrug-resistant tuberculosis. The serum small molecule metabolites in healthy controls, patients with drug-susceptible tuberculosis, and patients with multidrug-resistant tuberculosis were screened using ultra-high-performance liquid chromatography combined with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The differentially abundant metabolites were filtered out through multidimensional statistical analysis and bioinformatics analysis. Compared with drug-susceptible tuberculosis patients and healthy controls, the levels of 13 metabolites in multidrug-resistant tuberculosis patients altered. Among them, the most significant changes were found in N1-Methyl-2-pyridone-5-carboxamide (N1M2P5C), 1-Myristoyl-sn-glycerol-3-phosphocholine (MG3P), Caprylic acid (CA), and D-Xylulose (DX). And a multidrug-resistant tuberculosis/drug-susceptible tuberculosis differential diagnostic model was built based on these four metabolites, achieved the accuracy, sensitivity, and specificity of 0.928, 86.7%, and 86.7%, respectively. The enrichment analysis of metabolic pathways showed that the phospholipid remodeling of cell membranes was active in multidrug-resistant tuberculosis patients. In addition, in patients with tuberculosis, the metabolites of dipalmitoyl phosphatidylcholine (DPPC), a major component of pulmonary surfactant, were down-regulated. N1M2P5C, MG3P, CA, and DX may have the potential to serve as novel multidrug-resistant tuberculosis biomarkers. This research provides a preliminary experimental basis to further investigate potential multidrug-resistant tuberculosis biomarkers. Impact statement The MDR-TB incidence remains high, making the effective control of TB epidemic yet challenging. Rapid and accurate diagnosis is vitally important for improving the therapeutic efficacy and controlling the prevalence of drug resistance TB. Metabolomics has dramatic potential to distinguish MDR-TB and DS-TB. N1M2P5C, MG3P, CA, and DX that we identified in this study might have potential as novel MDR-TB biomarkers. The phospholipid remodeling of cell membranes was highly active in MDR-TB. The DPPC metabolites in TB were significantly down-regulated. This work aimed to investigate potential MDR-TB biomarkers to enhance the clinical diagnostic efficacy. The metabolic pathway distinctly altered in MDR-TB might provide novel targets to develop new anti-TB drugs.
    Type of Medium: Online Resource
    ISSN: 1535-3702 , 1535-3699
    URL: Article
    DOI: 10.1177/1535370220903464
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2020856-X
    SSG: 12
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  • 2
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    Brain deep medullary veins on 3-T MRI in a population-based cohort
    SAGE Publications ; 2021
    In:  Journal of Cerebral Blood Flow & Metabolism Vol. 41, No. 3 ( 2021-03), p. 561-568
    Details
    In: Journal of Cerebral Blood Flow & Metabolism, SAGE Publications, Vol. 41, No. 3 ( 2021-03), p. 561-568
    Abstract: Our aim is to investigate whether vascular risk factors are associated with cerebral deep medullary veins (DMVs) and whether DMVs are associated with MRI markers of cerebral small vessel disease (CSVD) or risk of stroke. In a community-based cohort of 1056 participants (mean age 55.7 years), DMVs were identified on susceptibility-weighted imaging (SWI) and counted in periventricular regions. Neuroimaging markers including lacunes, whiter matter hyperintensity (WMH), microbleeds, enlarged perivascular space, and brain atrophy were evaluated. The number of DMVs decreased with age (p = 0.007). After adjusting for age and sex, the number of DMVs was not associated with traditional vascular risk factors. Fewer DMVs was associated with increase of WMH and lacunes, but the association vanished after adjustment for vascular risk factors. However, fewer DMVs were independently associated with brain atrophy (p  〈  0.001). DMVs were not associated with three-year risk of stroke. Our results suggest that DMV is significantly different from other MRI markers of CSVD regarding risk factors, association with other CSVD markers, and risk of stroke. Nonetheless, the significant association between DMV and brain atrophy suggested the potential role of venules in age-related neurodegenerative process, which deserves further investigation.
    Type of Medium: Online Resource
    ISSN: 0271-678X , 1559-7016
    URL: Article
    DOI: 10.1177/0271678X20918467
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2039456-1
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  • 3
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    Deep medullary veins are associated with widespread brain structural abnormalities
    SAGE Publications ; 2022
    In:  Journal of Cerebral Blood Flow & Metabolism Vol. 42, No. 6 ( 2022-06), p. 997-1006
    Details
    In: Journal of Cerebral Blood Flow & Metabolism, SAGE Publications, Vol. 42, No. 6 ( 2022-06), p. 997-1006
    Abstract: Our aim is to investigate the association of cerebral deep medullary veins (DMVs) with white matter microstructural integrity and regional brain atrophy in MRI. In a community-based cohort of 979 participants (mean age 55.4 years), DMVs were identified on susceptibility-weighted imaging. Brain structural measurements including gray matter and hippocampus volumes, as well as diffusion tensor metrics, were evaluated. The mean (SD)number of DMVs was 19.0 (1.7). A fewer number of DMVs was related to lower fractional anisotropy and higher mean diffusivity in multiple voxels on the white matter skeleton (threshold-free cluster enhancement corrected p  〈  0.05, adjusted for age and sex). Also, fewer DMVs were significantly related to a lower gray matter fraction and a hippocampal fraction (0.10 and 0.11 per DMV, respectively; SE, 0.03 for both; p  〈  0.001 for both). A significant correlation between DMVs’ reduction and cortical atrophy was observed in the bilateral occipital lobes, temporal lobes, hippocampus, and frontal lobes (p  〈  0.001, adjusted for age, sex, and total intracranial volume). Our results provided evidence that cerebral small venules disease play a role in brain parenchymal lesions and neurodegenerative processes.
    Type of Medium: Online Resource
    ISSN: 0271-678X , 1559-7016
    URL: Article
    DOI: 10.1177/0271678X211065210
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2039456-1
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  • 4
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    Imaging angiogenesis using 68 Ga-NOTA-PRGD2 positron emission tomography/computed tomography in patients with severe intracranial atherosclerotic disease
    SAGE Publications ; 2017
    In:  Journal of Cerebral Blood Flow & Metabolism Vol. 37, No. 10 ( 2017-10), p. 3401-3408
    Details
    In: Journal of Cerebral Blood Flow & Metabolism, SAGE Publications, Vol. 37, No. 10 ( 2017-10), p. 3401-3408
    Abstract: Angiogenesis is a critical compensation route, which has been demonstrated in the brain following ischemic stroke; however, few studies have investigated angiogenesis in chronic intracranial atherosclerosis disease (ICAD). We used 68 Ga-NOTA-PRGD2 positron emission tomography/computed tomography based imaging to detect angiogenesis in chronic ICAD and to explore the factors that may have affected it. A total of 21 participants with unilateral severe chronic ICAD were included in the study. Of the 21 participants, 19 were men; the mean (SD) age was 52 (15) years. In 18 participants, we observed elevated 68 Ga-NOTA-PRGD2 uptake in the peri-infarct, subcortical, and periventricular regions of the lesioned side, with a higher 68 Ga-NOTA-PRGD2 SUV max compared to that in the contralateral hemisphere (0.15 vs. 0.06, p=0.001). The 18 F-FDG PET SUV max was significantly lower on the lesioned side (11.28 vs. 13.92, p=0.001). Subgroup analyses revealed that the recent group ( 〈 6 months) had a higher lesion-to-contralateral region ratio SUV max than the remote group ( 〉 6 months) (6.73 vs. 2.36, p 〈 0.05). Our results provide molecular imaging evidence of angiogenesis in patients with severe chronic ICAD. Furthermore, the extent of angiogenesis in chronic ICAD may be affected by the post-qualified event time interval, and not by infarction itself or the severity of the arterial lesion.
    Type of Medium: Online Resource
    ISSN: 0271-678X , 1559-7016
    URL: Article
    DOI: 10.1177/0271678X17696322
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2039456-1
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  • 5
    Online Resource
    Online Resource
    Tissue Engineering of Nanosilver-embedded Peripheral Nerve Scaffold to Repair Nerve Defects under Contamination Conditions
    SAGE Publications ; 2015
    In:  The International Journal of Artificial Organs Vol. 38, No. 9 ( 2015-09), p. 508-516
    Details
    In: The International Journal of Artificial Organs, SAGE Publications, Vol. 38, No. 9 ( 2015-09), p. 508-516
    Abstract: We employed a nanosilver-collagen scaffold and tested its effects on inhibiting bacteria and facilitating nerve regeneration. Methods Based on our previous research, we prepared bionic scaffolds with different concentrations of nanosilver and examined their internal structures by scanning electron microscopy and energy dispersive spectroscopy. We implanted these scaffolds or autologous nerve grafts into rats to repair a 10-mm injury of the sciatic nerve. Results The 2 mg/ml group showed a 〉 10 mm bacterial inhibition zone in all 3 types of bacterial culture dishes. At day 60 postsurgery, the 2 mg/ml group also showed the highest amplitude of evoked potential (AMP) and nerve conduction velocity (NCV). The regenerating nerves in the 2 mg/ml group were denser and more mature, and with thicker and well-arrayed myelin sheath. Conclusions These results demonstrate that nanosilver scaffolds (2 mg/ml group) were effective in inhibiting bacteria both in vitro and in vivo, and reduced the contamination-caused immune responses, which in turn promoted nerve regeneration and functional recovery.
    Type of Medium: Online Resource
    ISSN: 0391-3988 , 1724-6040
    URL: Article
    DOI: 10.5301/ijao.5000439
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 1474999-3
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  • 6
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    A Multi-Institutional Retrospective Analysis of Oncologic Outcomes for Patients With Locally Advanced Cervical Cancer Undergoing Platinum-Based Adjuvant Chemotherapy After Concurrent Chemoradiotherapy
    SAGE Publications ; 2021
    In:  Cancer Control Vol. 28 ( 2021-01-01), p. 107327482198930-
    Details
    In: Cancer Control, SAGE Publications, Vol. 28 ( 2021-01-01), p. 107327482198930-
    Abstract: To evaluated the oncologic outcomes associated with platinum-based adjuvant chemotherapy following concurrent chemoradiotherapy (CCRT) in the management of patients with locally advanced cervical cancer (LACC). Methods: A total of 695 patients with FIGO stage IB2, IIA2, IIB-IVA LACC treated at 6 medical facilities were enrolled and divided into 2 groups: 478 were assigned to CCRT alone (CCRT group) and 217 to adjuvant chemotherapy after CCRT (CCRT-ACT group). The treatment outcomes were retrospectively compared and reported after the propensity score matching (PSM) analysis. Results: With a median follow-up of 56.4 months, no statistically significant differences were found in overall survival (OS), disease-free survival (DFS), progression-free survival (PFS) and distance metastasis-free survival (DMFS) between 2 groups. In CCRT-ACT group, patients with lymph nodes involvement or squamous cell carcinoma (SCC) had significantly longer DMFS, but no significant benefit in survival outcomes were observed with more than 2 cycles of adjuvant chemotherapy. Moreover, patients with a high level of CA125 ( 〉 20.5U/mL) or SCC-Ag ( 〉 22.8μg/L) had a relatively better DFS or PFS, and grade 3-4 acute hematological toxicity, late urinary and lower gastrointestinal complications and diarrhea symptom were more frequent in CCRT-ACT group. Conclusions: Adjuvant chemotherapy after CCRT has a potential role in further improving disease control for LACC patients with lymph nodal-metastasis or SCC with a high level of CA125 or SCC-Ag. Due to increased treatment-related complications and diarrhea symptom affecting the quality of life, post-CCRT adjuvant chemotherapy with excessive cycles was not be considered as the most appropriate choice in general.
    Type of Medium: Online Resource
    ISSN: 1073-2748 , 1073-2748
    URL: Article
    DOI: 10.1177/1073274821989307
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2004182-2
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  • 7
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    Simultaneous determination of metolazone and valsartan in plasma by on-line SPE coupled with liquid chromatography/tandem mass spectrometry
    SAGE Publications ; 2017
    In:  European Journal of Mass Spectrometry Vol. 23, No. 5 ( 2017-10), p. 305-312
    Details
    In: European Journal of Mass Spectrometry, SAGE Publications, Vol. 23, No. 5 ( 2017-10), p. 305-312
    Abstract: Combination of metolazone (0.5 mg) and valsartan (80 mg) has been verified as a promising therapy treatment for hypertension. In order to facilitate to pharmacokinetic research, it needs a method for the simultaneously determination of metolazone and valsartan in biological samples. However, there are no relative reports so far. In order to facilitate to pharmacokinetic research, an on-line solid phase extraction coupled with liquid chromatography-tandem mass spectrometry method for the simultaneous determination of metolazone and valsartan in beagle dog plasma was developed and validated in this study. An on-line solid phase extraction column Retain PEP Javelin (10 mm × 2.1 mm) was used to remove impurities in plasma samples. The metolazone, valsartan and internal standard (losartan) were separated on a Poroshell 120 SB-C18 column (4.6 mm × 50 mm × 2.7 µm) with a gradient elution procedure. Acidified acetonitrile/water mixture was used as a mobile phase. The selected multiple-reaction monitoring mode in positive ion was performed and the parent to the product transitions m/z 366/259, m/z 436.2/291 and m/z 423.4/207 were used to measure the metolazone, valsartan and losartan. The method was linear over the range of 0.1–100 ng/mL and 1–1000 ng/mL for metolazone and valsartan, respectively. This method was validated in terms of specificity, linearity, sensitivity, precision, accuracy, matrix effect, and stability and then successfully applied to pharmacokinetic studies of the metolazone and valsartan combination tablets in beagle dogs.
    Type of Medium: Online Resource
    ISSN: 1469-0667 , 1751-6838
    URL: Article
    DOI: 10.1177/1469066717716726
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2021540-X
    detail.hit.zdb_id: 2021340-2
    SSG: 11
    SSG: 12
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  • 8
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    Prediction of microfibril angle in Dahurian larch wood using visible–near infrared spectroscopy and chemometric techniques
    SAGE Publications ; 2019
    In:  Journal of Near Infrared Spectroscopy Vol. 27, No. 4 ( 2019-08), p. 270-277
    Details
    In: Journal of Near Infrared Spectroscopy, SAGE Publications, Vol. 27, No. 4 ( 2019-08), p. 270-277
    Abstract: The microfibril angle of the S2 layer in the secondary cell wall of the tracheid is important for molecular and microscopic properties that influence collapse resistance, longitudinal modulus of elasticity and other lateral properties of conifers at the macroscopic level. This research aimed to investigate the feasibility of using a fruit fly optimization algorithm for visible and near infrared modeling optimization of Dahurian larch wood microfibril angle prediction. Originally, the linear relationship between microfibril angle and their raw spectra and visible and near infrared spectra pretreated by wavelet transform was established. Then, a nonlinear coupled model was built by combining the stepwise regression analysis and generalized regression neural network methods. As a final point, fruit fly optimization algorithm was used for optimizing stepwise regression analysis–generalized regression neural network coupled model. It was found that stepwise regression analysis–generalized regression neural network coupled model coupled model based on the optimization of fruit fly optimization algorithm simplify visible and near infrared spectral data and its prediction results ([Formula: see text] = 0.90, RMSEP = 0.75, mean average percentage error ([Formula: see text] ) = 0.05) outperforms original partial least squares model ([Formula: see text] = 0.86, RMSEP = 0.88, [Formula: see text]  = 0.06). This work demonstrated the feasibility of using improved chemometric techniques for improving the precision of visible and near infrared spectra in the prediction of microfibril angle.
    Type of Medium: Online Resource
    ISSN: 0967-0335 , 1751-6552
    URL: Article
    DOI: 10.1177/0967033519849725
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2019
    detail.hit.zdb_id: 2021280-X
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  • 9
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    Evaluating the impact of water protection policy on urban growth: A case study of Jiaxing
    SAGE Publications ; 2023
    In:  Environment and Planning B: Urban Analytics and City Science Vol. 50, No. 4 ( 2023-05), p. 1000-1019
    Details
    In: Environment and Planning B: Urban Analytics and City Science, SAGE Publications, Vol. 50, No. 4 ( 2023-05), p. 1000-1019
    Abstract: Source water protection can be a greater challenge in cities where the hydric resource is deeply embedded within a rapidly growing urban area. In these types of cities, the delimitation of protection areas along water resources, as one of the main mechanisms for water resource protection, has a direct impact on the way the urban form evolves. On the one hand, narrow protection areas may not be enough to guarantee that the built city does not affect water levels and quality. On the other hand, wide protection areas can result in a fragmented city, with low levels of accessibility and tendencies towards dispersed and disorderly growth. In this article we use the city of Jiaxing, China, as a case study to determine what the optimal size of water protection areas might be. For this, we use two urban growth models and simulate various urban growth scenarios between 2020 and 2040. The results indicate that a protection area of 400 m guarantees a good level of protection of the water resource and a sufficient availability of land that allows an efficient urban growth.
    Type of Medium: Online Resource
    ISSN: 2399-8083 , 2399-8091
    URL: Article
    DOI: 10.1177/23998083231163182
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2879402-3
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  • 10
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    Inhibition of Hepatitis B Virus Gene Expression and Replication by Helioxanthin and its Derivative
    SAGE Publications ; 2005
    In:  Antiviral Chemistry and Chemotherapy Vol. 16, No. 3 ( 2005-06), p. 193-201
    Details
    In: Antiviral Chemistry and Chemotherapy, SAGE Publications, Vol. 16, No. 3 ( 2005-06), p. 193-201
    Abstract: Chronic hepatitis B virus (HBV) infection continues to be an important worldwide cause of morbidity and mortality. All the currently approved therapeutic drugs have their limitations: interferon-α (IFN-α) has limited efficacy and a high incidence of adverse effects; nucleoside analogues are very efficient HBV DNA inhibitors, but resistance occurs eventually. Therefore, it is important to develop new non-nucleoside/nucleotide agents with different modes of action that can be used for antiviral combination therapy. Here, we report on a novel class of compounds, helioxanthin and its derivative 5-4-2, which had potent anti-HBV activities in HepG2.2.15 cells, with the EC 50 s of 1 and 0.08 μM, respectively. The lamivudine-resistant HBV, L526M/M550V double mutant strain, was also sensitive to helioxanthin and 5-4-2. This class of compounds not only inhibited HBV DNA, but also decreased HBV mRNA and HBV protein expression. The EC 50 of HBV DNA inhibition was consistent with the EC 50 of HBV 3.5 Kb transcript inhibition, which was 1 and 0.09 μM for helioxanthin and 5-4-2 respectively. Western blot analysis of cell lysate from HepG2.2.15 cells showed that the core protein expression decreased in a dose-dependent manner after drug treatment. In conclusion, helioxanthin and 5-4-2 are potentially unique new anti-HBV agents, which possess a different mechanism of action from existing therapeutic drugs. Both compounds inhibited HBV RNA and protein expression in addition to inhibiting HBV DNA.
    Type of Medium: Online Resource
    ISSN: 2040-2066 , 2040-2066
    URL: Article
    DOI: 10.1177/095632020501600305
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2005
    detail.hit.zdb_id: 2130088-4
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