In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 47, No. 6 ( 1974), p. 909-918
Abstract:
Fluorescein-tagged duck anti-rabbit cornea serum was used to demonstrate changes in the localization of metabolically dependent antigens (MDA) on surfaces of resting versus activated corneal endothelial cells. Actively growing cells in culture exhibited uniform membrane staining which was most pronounced at the periphery of the tissue cultures. In the metabolically less-active center, spotty rather than continuous fluorescence was seen. Cells with a very low metabolic activity, e.g., those kept at 4 °C, did not stain at all. This inhibition was reversible, as the cells transferred back to 37 °C were able to bind the antibodies. Cultured cells dispersed with pronase or trypsin reacted with the tagged antiserum and demonstrated membrane fluorescence. However, dispersed cells from excised normal cornea did not stain. The MDA, although not detectable on normal-cell surfaces, were present in these cells, for the isolated cell membrane fraction was capable of absorbing out antibodies to the MDA. The antigenic determinants involved in the reaction with fluorescein-tagged anti-tissue antibodies are thus present but masked in cell membranes of normal tissues 〈 i 〉 in vivo 〈 /i 〉 and can be unmasked by an increase in cellular metabolism and/or cell division but not by treatment with proteolytic enzymes.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
1974
detail.hit.zdb_id:
1482722-0
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