In:
Journal of Innate Immunity, S. Karger AG, Vol. 11, No. 1 ( 2019), p. 63-73
Abstract:
〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 We have previously identified the upregulation of the innate immune response, neutrophil activation, and apoptosis during anaphylaxis using a microarray approach. This study aimed to validate the differential gene expression and investigate protein concentrations of “hub genes” and upstream regulators during anaphylaxis. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Samples were collected from patients with anaphylaxis on their arrival at the emergency department, and after 1 and 3 h. mRNA levels of 11 genes ( 〈 i 〉 interleukin-6 [IL-6], IL-10, oncostatin M [OSM] , S100A8, S100A9, matrix metalloproteinase 9 [MMP9], FASL, toll-like receptor 4 [TLR4] , MYD88, triggering receptor expressed on myeloid cells 1 [TREM1] 〈 /i 〉 , and 〈 i 〉 cluster of differentiation 64 [CD64] 〈 /i 〉 ) were measured in peripheral blood leucocytes using qPCR. Serum protein concentrations were measured by ELISA or cytometric bead array for 6 of these candidates. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Of 69 anaphylaxis patients enrolled, 36 (52%) had severe reactions, and 38 (55%) were female. Increases in both mRNA and protein of IL-10, S100A9, MMP9, and TREM1 were observed. 〈 i 〉 OSM 〈 /i 〉 , 〈 i 〉 S100A8 〈 /i 〉 , 〈 i 〉 TLR4 〈 /i 〉 , and 〈 i 〉 CD64 〈 /i 〉 were upregulated and IL-6 protein concentrations were increased during anaphylaxis. Both 〈 i 〉 FASL 〈 /i 〉 and soluble Fas ligand decreased during anaphylaxis. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 These results provide evidence for the involvement of innate immune pathways and myeloid cells during human anaphylaxis, validating previous microarray findings. Elevated 〈 i 〉 S100A8 〈 /i 〉 , 〈 i 〉 S100A9 〈 /i 〉 , 〈 i 〉 TLR4 〈 /i 〉 , and 〈 i 〉 TREM1 〈 /i 〉 expression, and increased S100A9 and soluble TREM1 protein concentrations strongly suggest that neutrophils are activated during acute anaphylaxis.
Type of Medium:
Online Resource
ISSN:
1662-811X
,
1662-8128
Language:
English
Publisher:
S. Karger AG
Publication Date:
2019
detail.hit.zdb_id:
2455818-7
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