In:
Intervirology, S. Karger AG, Vol. 53, No. 4 ( 2010), p. 211-220
Abstract:
〈 i 〉 Objectives: 〈 /i 〉 To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered 〈 i 〉 Escherichia coli 〈 /i 〉 culture and termed engineered 〈 i 〉 E. coli 〈 /i 〉 phage (EEP). 〈 i 〉 Methods and Results: 〈 /i 〉 Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) and a long, flexible tail (138 nm in length). EEP was able to infect all 10 engineered 〈 i 〉 E. coli 〈 /i 〉 strains kept in our laboratory, showing a strong ability to lyse engineered 〈 i 〉 E. coli 〈 /i 〉 . Sequencing of the EEP genome revealed a double-stranded DNA (39.8 kb) with 54.72% GC content. Fifty-two open reading frames were predicted to be coding sequences, 18 of which were functionally defined and organized in a modular format, which includes modules for DNA replication, DNA packaging, structural proteins and host cell lysis. This phage could not be inactivated at 90° for 45 min and was resistant to ethanol and alkali treatment. EEP is assigned to the Siphoviridae family based on its morphological, genomic and physicochemical properties. 〈 i 〉 Conclusions: 〈 /i 〉 A novel coliphage was isolated from engineered 〈 i 〉 E. coli 〈 /i 〉 strains, and its morphological, genomic and physicochemical properties were characterized, which will improve our knowledge of bacteriophage diversity.
Type of Medium:
Online Resource
ISSN:
0300-5526
,
1423-0100
Language:
English
Publisher:
S. Karger AG
Publication Date:
2010
detail.hit.zdb_id:
1482863-7
SSG:
12
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