In:
Digestion, S. Karger AG, Vol. 62, No. 4 ( 2000), p. 262-270
Abstract:
〈 i 〉 Background: 〈 /i 〉 Human colorectal neuroendocrine cell carcinoma (NEC) is a rare disease with a poor prognosis. The biological behavior of NEC remains poorly understood. 〈 i 〉 Materials and Methods: 〈 /i 〉 We established two new NEC cell lines from a patient with rectal neuroendocrine carcinoma, NECS-P and NECS-L from the primary tumor and a liver metastasis, respectively. We investigated the biological differences between the two cell lines to study the mechanisms involved in liver metastasis. 〈 i 〉 Results: 〈 /i 〉 There was no difference between NECS-P and NECS-L in the morphological, ultrastructural and immunohistochemical studies. After addition of TGF-β 〈 sub 〉 1 〈 /sub 〉 , the doubling times of NECS-P were increased in a dose-dependent manner relative to untreated cells, whereas TGF-β 〈 sub 〉 1 〈 /sub 〉 had no effect on NECS-L. The attachment and chemotactic response of the two cell lines were not enhanced by TGF-β 〈 sub 〉 1 〈 /sub 〉 . The invasive capacity and the production of matrix metalloproteinase-2 (MMP-2) were significantly increased only in NECS-L following the addition of TGF-β 〈 sub 〉 1 〈 /sub 〉 . When anti-MMP-2 antibody was added to the medium with TGF-β 〈 sub 〉 1 〈 /sub 〉 , NECS-L invasion was inhibited. 〈 i 〉 Conclusion: 〈 /i 〉 It is considered that these differences are important to understand the mechanisms of liver metastasis of NEC.
Type of Medium:
Online Resource
ISSN:
0012-2823
,
1421-9867
Language:
English
Publisher:
S. Karger AG
Publication Date:
2000
detail.hit.zdb_id:
1482218-0
Permalink
