In:
Oncology, S. Karger AG, Vol. 95, No. 3 ( 2018), p. 179-187
Abstract:
〈 b 〉 〈 i 〉 Objective: 〈 /i 〉 〈 /b 〉 The involvement of epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC) has not been fully elucidated. Here, we aimed to identify EMT-related genes associated with TGF-β in ESCC and to clarify the role of these genes in the progression of ESCC. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 EMT-related genes associated with TGF-β expression were identified in patients with ESCC using microarray analysis and public datasets. The effects of ubiquitin-like with PHD and ring finger domains 2 (UHRF2) expression were analyzed in ESCC cell lines. Cell proliferation and invasion were measured using MTT and invasion assays, respectively. 〈 i 〉 UHRF2 〈 /i 〉 mRNA expression was also analyzed in 75 ESCC specimens to determine the clinical significance of 〈 i 〉 UHRF2 〈 /i 〉 in ESCC. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Treatment of ESCC cell lines with TGF-β increased UHRF2 expression. UHRF2 overexpression increased CDH1 (E-cadherin) expression and decreased invasive capacity. The 75 ESCC specimens were divided into the 〈 i 〉 UHRF2 〈 /i 〉 high-expression group ( 〈 i 〉 n 〈 /i 〉 = 61) and the 〈 i 〉 UHRF2 〈 /i 〉 low-expression group ( 〈 i 〉 n 〈 /i 〉 = 14). Low 〈 i 〉 UHRF2 〈 /i 〉 expression was significantly correlated with vascular invasion ( 〈 i 〉 p 〈 /i 〉 = 0.034) and was an independent prognostic factor for poor prognosis ( 〈 i 〉 p 〈 /i 〉 = 0.005). 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 UHRF2 may be a negative regulator of EMT and a novel prognostic biomarker for ESCC.
Type of Medium:
Online Resource
ISSN:
0030-2414
,
1423-0232
Language:
English
Publisher:
S. Karger AG
Publication Date:
2018
detail.hit.zdb_id:
1483096-6
detail.hit.zdb_id:
250101-6
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