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  • S. Karger AG  (4)
  • Medicine  (4)
  • XA 49650  (4)
  • 1
    Online Resource
    Online Resource
    Mutational Analysis of Amino Acid Positions Crucial for IgE-Binding Epitopes of the Major Apple 〈i〉(Malus domestica)〈/i〉 Allergen, Mal d 1
    S. Karger AG ; 2006
    In:  International Archives of Allergy and Immunology Vol. 139, No. 1 ( 2006), p. 53-62
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 139, No. 1 ( 2006), p. 53-62
    Abstract: 〈 i 〉 Background: 〈 /i 〉 Individual amino acid residues of the major birch pollen allergen, Bet v 1, have been identified to be crucial for IgE recognition. The objective of the present study was to evaluate whether this concept was applicable for the Bet v 1-homologous apple allergen, Mal d 1. 〈 i 〉 Methods: 〈 /i 〉 A Mal d 1 five-point mutant was produced by PCR techniques, cloned into pMW 172 and expressed in 〈 i 〉 Escherichia coli 〈 /i 〉 BL21(DE3) cells. To evaluate the allergenic properties of the engineered protein compared to Mal d 1 wild-type IgE immunoblotting, ELISA, peripheral blood monocytes proliferation assays, and skin prick tests were performed. 〈 i 〉 Results: 〈 /i 〉 The Mal d 1 mutant showed reduced capacity to bind specific IgE as compared to wild-ype Mal d 1 in in vitro assays in the majority of the sera tested. In ELISA, 10 out of 14 serum samples displayed an 88–30% decrease in IgE binding to Mal d 1 mutant compared to wild-type Mal d 1. Skin prick tests in apple-allergic patients (n = 2) confirmed the markedly decreased ability of the Mal d 1 mutant to induce allergic reactions in vivo. However, the relevant T cell epitopes were present in the mutated molecule according to peripheral blood mononuclear cell proliferation assays. 〈 i 〉 Conclusions: 〈 /i 〉 Our findings suggest that it is possible to modulate the IgE-binding properties of allergens by single amino acid substitutions at crucial positions which might be useful for future immunotherapy of birch-pollen-associated food allergies which are not ameliorated by birch pollen immunotherapy.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000089756
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2006
    detail.hit.zdb_id: 1482722-0
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  • 2
    Online Resource
    Online Resource
    An Antagonist for CCR4 Alleviates Murine Allergic Rhinitis by Intranasal Administration
    S. Karger AG ; 2012
    In:  International Archives of Allergy and Immunology Vol. 159, No. 3 ( 2012), p. 297-305
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 159, No. 3 ( 2012), p. 297-305
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 CCR4 is highly expressed on Th2 cells. These cells play an important role in acute inflammatory responses, including those involved in allergic rhinitis. We determined whether disrupting the CCR4 ligand interaction with CCR4 antagonist could alleviate allergic rhinitis in a mouse model. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 BALB/c mice were sensitized with ovalbumin and alum by intraperitoneal injection and challenged with intranasally administered ovalbumin. Compound 22, which has been reported as a novel small-molecule antagonist of CCR4, was also administered intranasally. In addition, budesonide, an efficient glucocorticoid, was used as a positive control. The effects of compound 22 were quantified by multiple parameters of allergic responses in both nasal and pulmonary tissues. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Compound 22 significantly improved symptoms of allergic rhinitis and suppressed levels of total IgE of serum. It dramatically reduced the levels of IL-4 in bronchoalveolar lavage fluid and also decreased the number of inflammatory cells in the fluid. The infiltration of inflammatory cells, especially eosinophils, was markedly reduced in the nasal and pulmonary tissues. The number of IL-4+ cells was also significantly reduced in these tissues. Moreover, the numbers of Foxp3+ cells and IL-17+ cells were reduced, though not to a statistically significant degree. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 In our research, CCR4 antagonists such as compound 22 were proven for the first time to alleviate murine allergic rhinitis when administered nasally. CCR4 antagonists may have therapeutic potential for the treatment of allergic rhinitis.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000337455
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2012
    detail.hit.zdb_id: 1482722-0
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  • 3
    Online Resource
    Online Resource
    The Protective Effects of 〈b〉〈i〉Helicobacter pylori〈/i〉〈/b〉 Infection on Allergic Asthma
    S. Karger AG ; 2021
    In:  International Archives of Allergy and Immunology Vol. 182, No. 1 ( 2021), p. 53-64
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 182, No. 1 ( 2021), p. 53-64
    Abstract: As an ancient Gram-negative bacterium, 〈 i 〉 Helicobacter pylori 〈 /i 〉 has settled in human stomach. Eradicating 〈 i 〉 H. pylori 〈 /i 〉 increases the morbidities of asthma and other allergic diseases. Therefore, 〈 i 〉 H. pylori 〈 /i 〉 might play a protective role against asthma. The “disappearing microbiota” hypothesis suggests that the absence of certain types of the ancestral microbiota could change the development of immunology, metabolism, and cognitive ability in our early life, contributing to the development of some diseases. And the Hygiene Hypothesis links early environmental and microbial exposure to the prevalence of atopic allergies and asthma. Exposure to the environment and microbes can influence the growing immune system and protect subsequent immune-mediated diseases. 〈 i 〉 H. pylori 〈 /i 〉 can inhibit allergic asthma by regulating the ratio of helper T cells 1/2 (Th1/Th2), Th17/regulatory T cells (Tregs), etc. 〈 i 〉 H. pylori 〈 /i 〉 can also target dendritic cells to promote immune tolerance and enhance the protective effect on allergic asthma, and this effect relies on highly suppressed Tregs. The remote regulation of lung immune function by 〈 i 〉 H. pylori 〈 /i 〉 is consistent with the gut-lung axis theory. Perhaps, 〈 i 〉 H. pylori 〈 /i 〉 also protects against asthma by altering levels of stomach hormones, affecting the autonomic nervous system and lowering the expression of heat shock protein 70. Therapeutic products from 〈 i 〉 H. pylori 〈 /i 〉 may be used to prevent and treat asthma. This paper reviews the possible protective influence of 〈 i 〉 H. pylori 〈 /i 〉 on allergic asthma and the possible application of 〈 i 〉 H. pylori 〈 /i 〉 in treating asthma.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000508330
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2021
    detail.hit.zdb_id: 1482722-0
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  • 4
    Online Resource
    Online Resource
    Use of Epinephrine in Patients with Drug-Induced Anaphylaxis: An Analysis of the Beijing Pharmacovigilance Database
    S. Karger AG ; 2017
    In:  International Archives of Allergy and Immunology Vol. 173, No. 1 ( 2017), p. 51-60
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 173, No. 1 ( 2017), p. 51-60
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Few studies assessing the use of epinephrine in drug-induced anaphylaxis (DIA) in the hospital setting are available. We utilized the Beijing Pharmacovigilance Database (BPD) to evaluate the appropriateness of epinephrine for DIA management. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 DIA cases collected in the BPD from January 2004 to December 2014 were adjudicated and analyzed for demographics, causative drugs, clinical signs, outcomes, initial treatment, route, dosing, and cardiovascular adverse events (CAE) of epinephrine. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 DIA was primarily caused by antibiotics (38.4%), radiocontrast agents (11.9%), traditional Chinese medicine injections (10.9%), and chemotherapeutic drugs (10.3%). Only 708 (59.5%) patients received epinephrine treatment. Patients who received epinephrine were more likely to experience wheezing ( 〈 i 〉 p 〈 /i 〉 〈 0.001) and respiratory arrest ( 〈 i 〉 p 〈 /i 〉 〈 0.001). Among 518 patients with a complete record of the epinephrine administration route, the percentage of patients receiving it by intramuscular (IM) injection, subcutaneous (SC) injection, intravenous (IV) bolus injection, or IV continuous infusion was 16.9, 31.5, 43.5, and 8.1%, respectively. Among the 427 patients with a record of both the administration route and the dosing, an overdose was more likely with IV bolus (94.1%) in contrast to IM injection (56.6%; 〈 i 〉 p 〈 /i 〉 〈 0.001) or SC injection (43.7%; 〈 i 〉 p 〈 /i 〉 〈 0.001). Among the patients analyzed for CAE ( 〈 i 〉 n 〈 /i 〉 = 349), 17 patients accounted for 19 CAE, and 13 (76.5%) of these patients were overdosed with epinephrine. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 Underuse, inappropriate IV bolus use, and overdosing were the 3 major problems with epinephrine use in DIA in China. Educational training for health care professionals on the appropriate use of epinephrine in managing anaphylactic reactions is suggested.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000475498
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2017
    detail.hit.zdb_id: 1482722-0
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