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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (34)
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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (34)
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  • 1
    Online Resource
    Online Resource
    EFFECTS OF SALT STRESS ON PLANT GROWTH AND BIOMASS ALLOCATION IN SOME WETLAND GRASS SPECIES IN THE MEKONG DELTA
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Science and Technology Vol. 58, No. 3A ( 2020-05-25), p. 50-
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 58, No. 3A ( 2020-05-25), p. 50-
    Abstract: Salt stress causes serious damage to many cellular and physiological processes that leads to yield reduction. The study induced salt stress using Hoagland solution added NaCl to evaluate its effects on plant growth and biomass allocation of some wetland grass species in order to identify salt-tolerant species for replacing and/or supplementing rice/grass in rice-shrimp model and salt-affected area in the Mekong Delta. The study also seeks to evaluate the response of leaf chlorophyll (SPAD unit) and proline content in salt-treated plants to varying application of salinity. Typha orientalis, Lepironia articulata, Eleocharis dulcis and Scirpus littoralis were studied in hydroponics condition with four levels of NaCl of 5, 10, 15, 20‰ and the control treatment (without adding NaCl). The experiment was arranged in a completely randomized design with 3 replications. The salt-treated plants showed visually clear responses of inhibited growth under salt stress condition compared to the control plants. Among the four studied species, T. orientalis produced the highest dry shoot biomass (15.5 g DW/plant), while E. dulcis had the lowest value (2.8 g DW/plant). However, only T. orientalis showed significantly decreased in biomass as salinity increased with 9.3 and 4.6 times lower of fresh and dry biomass in plants grown at the salinity level of 20‰ compared to those grown in the control treatment. The other three plant species did not affect by salinity levels. The results indicated that S. littoralis, L. articulata and E. dulcis could tolerate at high salinity of 20‰ (eq. to the EC value in the nutrient solution of 38.0 dS/m) and could be potential candidate to grow in the rice-shrimp model or in the salt-affected soils.  
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/58/3A/14251
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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  • 2
    Online Resource
    Online Resource
    DẪN LIỆU MỚI VỀ MÔI TRƯỜNG NƯỚC VÀ SINH VẬT TRONG CÁC HANG NGẦM VÀ HỒ NƯỚC MẶN KHU VỰC VỊNH HẠ LONG, CÁT BÀ
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  Vietnam Journal of Marine Science and Technology Vol. 16, No. 2 ( 2016-06-30), p. 167-175
    Details
    In: Vietnam Journal of Marine Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 2 ( 2016-06-30), p. 167-175
    Abstract: Hang ngầm và hồ nước mặn là 2 dạng sinh cảnh khá phổ biến ở Hạ Long và Cát Bà, do nhiều nguyên nhân khác nhau đến nay chúng rất ít được quan tâm nghiên cứu. Các kết quả nghiên cứu bước đầu về môi trường và quần xã sinh vật trong 3 hang ngầm (Hang Sáng, Hang Tối, Hang Quả Bàng) và 3 hồ nước mặn (Áng Đầu Bê, Áng Dù, Áng Quả Bàng) cho thấy: Môi trường nước có sự khác biệt giữa các hồ, đặc biệt là trong hồ kín như Áng Dù có độ muối thấp (9‰), trong khi các hồ có cửa thông với biển có độ muối gần tương đương với môi trường ngoài (23 - 27‰). Hàm lượng chất khí hòa tan như DO trong các hồ nước mặn khá cao từ 7,63 - 9,03 mg/l, cao hơn trong hang ngầm và cao hơn ở môi trường biển xung quanh. Các yếu tố vật lý và hóa học trong môi trường nước tại các hang ngầm gần tương đương với môi trường bên ngoài do có các hang ngầm đều thông với biển và có nước chảy thường xuyên theo sự lên xuống của thủy triều. Quần xã sinh vật trong các hang khá phong phú với trên 142 loài được tìm thấy, phổ biến nhất là hải miên và san hô mềm, chúng phân bố dọc chiều dài hang. Có một số loài có giá trị kinh tế cao thường gặp trong hang là Cù kì Myomenippe hardwickii, ghẹ Portunus pelagicus, ốc nón Trochus pyramis, cá dìa Siganus sutor, cá hồng Lutjanus russellii ... Chưa phát hiện thấy các loài chuyên biệt sống cố định trong hang. Ở các hồ nước lưu thông với môi trường bên ngoài có sự xuất hiện của rạn san hô, chúng tạo thành một dải hẹp bao quanh hồ. Các bãi cát thường xuất hiện quanh hồ ở độ sâu 0,5 - 2 m có các loài đặc sản như phi phi, tu hài, hải sâm với mật độ khá cao (Áng Đầu Bê, Áng Quả Bàng). Trong áng kín không có rạn san hô do nước có độ muối thấp, có sự phân tầng của nhiệt độ và độ muối làm cho nhiệt độ ở tầng mặt thấp hơn tầng đáy 3 - 60C đây là hiện tượng bất thường ở các hồ này.
    Type of Medium: Online Resource
    ISSN: 1859-3097
    URL: Article
    DOI: 10.15625/1859-3097/16/2/7341
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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  • 3
    Online Resource
    Online Resource
    Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 2 ( 2016-06-30), p. 287-293
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 2 ( 2016-06-30), p. 287-293
    Abstract: The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/14/2/9354
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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  • 4
    Online Resource
    Online Resource
    STUDYING REACTIVITY RATIOS AND PHYSICAL PROPERTIES OF METHACRYLIC ACID - ETHYL ACRYLATE COPOLYMER
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2015
    In:  Journal of Science and Technology Vol. 53, No. 2 ( 2015-04-22)
    Details
    In: Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 53, No. 2 ( 2015-04-22)
    Type of Medium: Online Resource
    ISSN: 0866-708X , 0866-708X
    URL: Article
    DOI: 10.15625/0866-708X/53/2/4002
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2015
    SSG: 11
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  • 5
    Online Resource
    Online Resource
    Electrochemical Fabrication of Hybrid Plasmonic-dielectric Nanomaterial Based on Gold-diamond Clusters
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2017
    In:  Communications in Physics Vol. 27, No. 1 ( 2017-02-22), p. 37-
    Details
    In: Communications in Physics, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 27, No. 1 ( 2017-02-22), p. 37-
    Abstract: Hybrid plasmonic-dielectric material were fabricated by micro-discharge through water sols of sub-micrometer-sized diamonds mixed with HAuCl4 acid. Primary characterization of their deposits on a silicon wafer surface by means of electron microscopy and energy-dispersive x-ray spectroscopy indicate close proximity of gold nanoparticles and diamond particles, which is supported by photoluminescence studies demonstrating strong – almost two-fold – damping of diamond luminescence owing to the attachment of gold nanoparticles. UV-near IR spectroscopy of their sols consistently exhibits small red spectral shifts for the fabricated nanomaterial, comparing to bare gold nanoparticles.
    Type of Medium: Online Resource
    ISSN: 2815-5947 , 0868-3166
    URL: Issue
    URL: Article
    DOI: 10.15625/0868-3166-27-1
    DOI: 10.15625/0868-3166/27/1/8886
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2017
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  • 6
    Online Resource
    Online Resource
    Protection of chickens against A/H5N1 virus by plant-based haemagglutinin fused IGMFC
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 3 ( 2020-11-28), p. 477-485
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 3 ( 2020-11-28), p. 477-485
    Abstract: Vaccination is one of the most effective and cost-beneficial interventions for protection of animals against the highly pathogenic A/H5N1 avian influenza virus. Haemagglutinin (HA) is a transmembrane glycoprotein of A/H5N1 virus and is a critical antigen for development of the influenza vaccine. The haemagglutinin-based vaccine produced in plants was demonstrated as a candidate vaccine since it elicited neutralizing antibodies against A/H5N1 virus. In this study, immunogenicity and protective ability of a plant-based recombinant HA antigen which was fused to IgMFc to form oligomerized HA antigen (H5TG oligomer) had been evaluated by vaccination in chickens. Chicken sera after each vaccination were collected for Western blot, ELISA and HI assays. Ten days after the second vaccination, the chickens have been challenged with A/duck/TG/NAVET(3)/2013 virus, clade 1.1. The analysis results showed that the oligomeric recombinant H5TG antigen elicited stronger H5TG-specific IgY antibodies and A/H5N1 clade 1.1 virus-neutralizing antibodies than the H5TGpII trimeric recombinant antigen without fusing IgMFc in vaccinated chickens. Notably, the chicken protection rate against A/H5N1 clade 1.1 virus of the H5TG oligomer antigen was 80% that is not significantly lower than that of a commercial vaccine as a positive control from National Veterinary Joint Stock company NAVETCO, Vietnam with the chicken protective rate of 90%. Whereas the chicken protection rate against A/H5N1 clade 1.1 virus of the H5TG trimer antigen was 50%. These results suggest that the IgMFc motif plays an important role in the forming of oligomeric proteins which had been proved for enhancing immunogenicity and protection ability in this study. Therefore, the plant-based oligomerized recombinant H5TG antigen is a potential vaccine candidate against A/H5N1 influenza virus in the future.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/3/14483
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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  • 7
    Online Resource
    Online Resource
    A study on screening of cholesterol-lowering lactic acid bacteria
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2014
    In:  TAP CHI SINH HOC Vol. 36, No. 1se ( 2014-08-07)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 36, No. 1se ( 2014-08-07)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v36n1se.4361
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2014
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  • 8
    Online Resource
    Online Resource
    Genus mallotus (euphorbiaceae): a review on traditional medicinal use, phytochemistry and biological activities
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Science and Technology Vol. 60, No. 2 ( 2022-04-21), p. 141-174
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 60, No. 2 ( 2022-04-21), p. 141-174
    Abstract: Ethnopharmacological relevance: The genus Mallotus (Euphorbiaceae family) includes 124 accepted names and distributes in tropical and sub-tropical regions. Some Mallotus species are used in traditional medicines for the treatment of chronic hepatitis, enteritis stomach, disorders, cholelithiasis, anti-cancer,  gastric, and duodenal ulcers. Aim of the study: According to the Scifinder database, this is the first review study that focuses on the phytochemistry and pharmacology of Mallotus genus (17 Mallotus species) to understand the link between the traditional medicinal uses, phytochemistry, and bioactivities. Thus, they provides a scientific fundamental for further research in the phytochemical and pharmacological activities of their species. Materials and methods: Information about the Mallotus genus was collected using various databases, such as Web of Science, SciFinder, PubMed, Sci-hub, Google Scholar, Wiley, Elsevier, ACS publications, and SpringerLink between 1978 and 2021. Plant names were validated by “The Plant List” (www.theplantlist.org).  Results: Up to now, total 325 compounds were reported from Mallotus species, including phloroglucinols, steroids, coumarins, benzopyrans, flavonoids, chalcones, galics, and bergenins. The extracts and phytochemical constituents of the Mallotus genus are a rich source of biologically active, including anti-cancer, anti-inflammatory, immunoregulatory, antioxidant, antibacterial, antifungal, anti-virus, and other activities. Conclusions: The review indicated that the Mallotus genus is a promising source of biological activities compounds, special anti-cancer chromanes. The results of this review confirm the great potential of Mallotus species. Thus, it will be helpful for further research in the phytochemistry and pharmacology of the Mallotus genus.
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/16634
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
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  • 9
    Online Resource
    Online Resource
    EFFECT OF CULTURE CONDITIONS FOR ANTIMICROBIAL ACTIVITY OF MARINE - DERIVED FUNGUS ASPERGILLUS FLOCCULOSUS 01NT.1.1.5
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 15, No. 4 ( 2018-12-14), p. 721-728
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 15, No. 4 ( 2018-12-14), p. 721-728
    Abstract: The biosynthesis of compounds with antibiotic activity produced by marine fungi, strongly depends on their growth conditions. A good understanding of the role of culture conditions in the biosynthesis of metabolites may lead to better exploitation of microbial metabolites. In this study, the influence of culture conditions including incubation period, initial pH and salinity on antimicrobial activity and secondary metabolites production of marine fungus 01NT.1.1.5 was investigated. This isolate, obtained from sponge Stylissa sp. in Nha Trang Bay, exhibited a broad spectrum of in vitro antimicrobial activity to Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Listeria monocytogenes ATCC 19111, Streptococcus faecalis ATCC 19433 and Candida albicans ATCC 10231. According to morphological characteristics and sequence analysis of 28S rDNA, the fungus was identified as Aspergillus flocculosus. The results indicated that antimicrobial activity and metabolite amount were highest when the fungus was cultivated in rice medium with incubation period of 20 days. The optimum salinity of 35 g/L and initial pH of 6.0 were found for the maximum antibiotic production. The colony growth, antimicrobial activity and production of secondary metabolites of the strain A. flocculosus 01NT.1.1.5 varied depending on salt concentrations and initial pH of medium. Particularly, extract of this fungus only showed activity against C. albicans when it was cultured in medium with 30-35 g/L salinity and initial pH 4.0-8.0. The results  indicate that salinity and initial pH along with cultivation period are important factors influencing antimicrobial activity and secondary metabolites of A. flocculosus 01NT.1.1.5, and might be for other marine fungi.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15/4/13415
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 10
    Online Resource
    Online Resource
    Bio-functional enhancement of plant based-haemagglutinin by fusion with IgMFc
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 2 ( 2020-11-03), p. 293-305
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 2 ( 2020-11-03), p. 293-305
    Abstract: The creation of recombinant oligomeric haemagglutinin proteins from A/H5N1 virus is a new concern for many scientists. In this study, the gene encoding the haemagglutinin protein (H5TG) derived from virus A/duck/Vietnam/TG24-01/2005 was fused with three different motifs (GCN4pII, GCN4pII-IgMFc and GCN4pII-ELP-IgMFc) in order to form three recombinant proteins (trimeric H5TGpII, oligomeric H5TGpII-IgMFc and ELPylated oligomeric H5TGpII-ELP-IgMFc, respectively) for enhancing protein expression, bio-function and purification of H5TG. These H5TG fragments have been attached to expression cassettes in the pCB301 shuttle vector, transiently expressed in Nicotiana benthamiana by agroinfiltration, then the protein expression was confirmed by SDS-PAGE and Western blot analysis. The results showed that the expression level of the H5TGpII-ELP-IgMFc protein in plant raw extract was stronger than that of two other proteins analyzed in this paper. Evaluation of bio-function showed that the haemagglutination titre (HA titre) of the total solution protein extract containing H5TGpII-IgMFc protein was highest (64 HAU) compared to that containing two remaining proteins (8 HAU). Subsequently, the H5TGpII and H5TGpII-IgMFc proteins were purified by immobilized metal ion affinity chromatography (IMAC), while the H5TGpII-ELP-IgMFc protein was purified using membrane-based Inverse Transition Cycling (mITC). The oligomer state of the purified proteins was then determined by non-reducing SDS-PAGE. The haemagglutination assay analysis of purified proteins showed that the lowest protein amount causing erythrocyte agglutination (1 HAU) of the H5TGpII-IgMFc protein was 0.06 µg and lower four times than that of H5TGpII and H5TGpII-ELP-IgMFc proteins (both of them were 0.24 µg). This indicates that the fusing of the GCN4pII-IgMFc motif into the H5TG protein gives the stronger bio-function than the fusing of two remaining motifs into this protein. This result opens up the applicability of the IgMFc for generation of oligomer proteins to enhance the bio-function of target proteins in the study of recombinant vaccine production.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/2/14479
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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