GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (277)
Material
Publisher
  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (277)
Person/Organisation
Language
Years
FID
  • 1
    Online Resource
    Online Resource
    De novo mutations of \(\textit{ ELANE}\) gene in three Vietnamese patients with severe congenital neutropenia
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Academia Journal of Biology Vol. 44, No. 3 ( 2022-09-28), p. 77-85
    Details
    In: Academia Journal of Biology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 44, No. 3 ( 2022-09-28), p. 77-85
    Abstract: Severe congenital neutropenia (SCN) is a congenital condition in which granulocytes mature abnormally owing to a variety of genetic defects, resulting in immunodeficiency. Among the several genetic variations related to SCN, heterozygous mutations in the ELANE gene encoding neutrophil elastase account for approximately 60% of the genetic causes. Here, we present three patients from different Vietnamese families who were susceptible to infectious diseases such as lung abscesses, sepsis, cellulitis, and septicemia. Moreover, their hematological and immunological parameters were below the reference range. Whole exome sequencing (WES) analysis was performed in all cases harboring three previously described disease-causing mutations, including p.Arg103Pro, p.Trp156Arg, and p.Arg81Pro in the ELANE gene (NM_001972.4). These mutations were confirmed by the Sanger sequencing method in the patients, helping to identify de novo mutations in all cases. Our data increase more evidence for the function of ELANE in SCN, as well as raise awareness of this rare disease in the context of frequent infections in Vietnam.
    Type of Medium: Online Resource
    ISSN: 2615-9023 , 2615-9023
    URL: Article
    DOI: 10.15625/2615-9023/17080
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Identification of the causative mutation in the ITGB2 gene in a LAD1 patient by whole exome sequencing
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 20, No. 2 ( 2022-06-30), p. 213-218
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 20, No. 2 ( 2022-06-30), p. 213-218
    Abstract: Leukocyte adhesion deficiency type 1 (LAD1) is a rare congenital immunodeficiency disease. The cause of disease is determined to be the mutations in the ITGB2 gene that codes for CD18, the beta chain of beta-2 integrins, leads to decreased expression or functioning of CD18. This deficiency leads to severe impairment of leukocyte adhesion to the vascular wall and leukocyte migration to sites of infection and inflammation. LAD1 has also been associated with inhibition of interleukin-23 and interleukin-17 resulting in a hyperinflammatory and chronic inflammation. Patients with LAD1 typically present in early infancy with recurrent, life threatening infections that are frequently fatal before 2 years of age  without hematopoietic stem cell transplant (HSCT). However, LAD1 is difficult to diagnose and many LAD1 patients die at a young age despite intensive antibiotic therapy. Accurate diagnosis requires detailed clinical information (delayed umbilical cord loss, severe periodontitis, delayed wound healing and sores, skin abscesses, and recurrent infection), and confirmation the absence of integrins by flow cytometric analysis. A better understanding of the molecular characteristics of this disease is needed to raise awareness and definitive diagnosis infants with LAD1. To definitive diagnosis, whole exome sequencing and Sanger sequencing were performed in an eighteen-month-old boy with severe leukocytosis, recurrent infections, delayed wound healing, and hepatosplenomegaly associated with an acquired cytomegalovirus infection. Two variants: One previously reported mutation (c.533C 〉 T, p.Pro178Leu) and one novel variant (c.59-1G 〉 A), in the ITGB2 gene were detected. These results can be used for definitive genetic diagnosis, genetic counseling, as well as a prenatal diagnosis in LAD1 patients.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15886
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Whole-exome sequencing as a diagnostic tool for ipex syndrome
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Academia Journal of Biology Vol. 44, No. 1 ( 2022-03-30), p. 53-60
    Details
    In: Academia Journal of Biology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 44, No. 1 ( 2022-03-30), p. 53-60
    Abstract: Immune dysregulation-Polyendocrinopathy-Enteropathy-X-linked (IPEX) syndrome is a life-threatening congenital autoimmune disorder caused by mutations in the forkhead box protein 3 (FOXP3) gene. Typical clinical manifestations of IPEX patients are early onset of intractable diarrhea, type 1 diabetes mellitus, and skin diseases. However, other autoimmune types such as severe food allergies, autoimmune cytopenias, autoimmune respiratory illness, and mesangial glomerulonephritis may complicate IPEX diagnosis. In this study, we report a Vietnamese 1-year-old boy with IPEX syndrome due to a hemizygous missense mutation, c.1190G 〉 A (p.Arg397Gln), in exon 12 of the FOXP3 gene (NM_014009.4). The child had dermatitis, diarrhea, respiratory infections, and splenomegaly. The patient's serum routine test results were expected, except for white blood cells and neutrophils were higher than the normal, while IgA concentration was slightly below the normal range. However, he got no signal of diabetes or failure to thrive. Whole exome sequencing was applied to identify a genetic variant, and variant validation was examined using Sanger sequencing. The patient’s genetic mutation was inherited from his mother, an obligate carrier. His father had a normal genotype. This study is the first report of IPEX syndrome in a Vietnamese patient with a mutation in the FOXP3 gene detected by WES. This study provides further evidence for the role of mutations in the FOXP3 gene in patients with IPEX syndrome and demonstrates the need for genetic counselling and prenatal testing. Our results also show that WES sequencing is an effective tool in diagnosing genetic diseases.
    Type of Medium: Online Resource
    ISSN: 2615-9023 , 2615-9023
    URL: Article
    DOI: 10.15625/2615-9023/16305
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Detection of DNA of Rickettsia and Orientia tsutsugamushi in rodents and ectoparasites in Ha Giang province
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 3 ( 2020-11-28), p. 543-552
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 3 ( 2020-11-28), p. 543-552
    Abstract: Rickettsial fever is one of a zoonotic disease which is caused by bacteria genus Rickettsia. The ectoparasites such as ticks, mites, fleas, lice... were demonstrated as the main transmited vectors through host reservoirs are rodents and small animals including mice, squirrels, mink... In this study, the rodents and ectoparasites species were identified. The molecular detection of Rickettsia was also performed. In 2018, 83 rats were trapped in 2 villages Thanh Duc and Phu Linh, Vi Xuyen district, Ha Giang province, in which 48.2% mice were found as house mice Rattus flavipectus, 21.7% was forest mice R. rattus, 12% was R. fulvescens, 8.4% was R. nitidus, the remaining rates were R. bowersi, Mus. pahari, Leopoldamys sabanus, Mus musculus and R. niviventer, accounting for 1.2% - 3.6%. The ectoparasites survey found 5 chigger mite species including Leptotrombidium (Leptotrombidium) deliense, Ascoschoengastia (Laurentella) indica, Garhliepia (Walchia) rustica, Lorilutum oreophilum and Shunsenia sp as well as 3 gamasid mite species such as Laelaps (Echidninus) sedlaceki, Laelaps (Laelaps) nuttali and Lenstivalius klossi bispiniformis. The result indicated that 19.3% and 10.8% mice were positive with Ricketsia spotted fever group (SFG) and Rickettsia typhi, respectively by real-time PCR. The nested PCR result showed that 19.4% R. flavipectus mice and 10% L. (L.) deliense chigger mites were positive with Orientia tsutsugamushi. 
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/3/13892
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Application of fluorescence microscopy for histological discrimination of golden camellias leaves
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2023
    In:  Vietnam Journal of Biotechnology ( 2023-03-22)
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), ( 2023-03-22)
    Abstract: Histological analysis, which aims to investigate the microscopic anatomy of biological tissues, has been a simple and powerful technique for plant taxonomy. Sectioning followed by staining methods is widely used in observing histological structures. However, the staining techniques often destroy tissue and provide low-quality images due to nonspecific reactions with the dyes making further analysis difficult. In this report, we propose an applicable non-staining histology protocol based on auto-fluorescence characteristics of plant tissues and its application in the anatomical discrimination of six similar-appearance species of golden camellias as a case study. We compared the images from the same tissue under a bright field with the staining step and under fluorescence directly without the staining step in the sample preparation. The images were taken from Eclipse Ni-U microscopy (Nikon, Japan) with a color DS-Ri2 camera (Nikon, Japan) and NIS-ELEMENTS Basic Research Imaging software. The non-staining method demonstrated significant advantages compared to the staining protocol. The fluorescent images showed the distinction between adjacent leaf tissues with their own naturally reflective colors. In addition, the anatomical parameters, including the xylem area, phloem area, bundle sheath area, and palisade/spongy width ratio, were easily measured in good-quality images. These parameters were used in discriminative analysis by the Principal Component Analysis (PCA). The PCA diagram demonstrated the separation of six species, thus suggesting that these anatomical parameters can be used for taxonomy. In conclusion, our study showed a helpful technique in histological analysis that significantly contributes to the taxonomy of golden camellias species and can be applied in other plant varieties.
    Type of Medium: Online Resource
    ISSN: 2815-5912 , 2815-5955
    URL: Article
    DOI: 10.15625/1811-4989/17967
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2023
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Chemical compounds of esential oils from Xyloselinum vietnamense Pimenov & Kljuykov and Xyloselinum leonidii Pimenov & Kljuykov in Vietnam
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2013
    In:  TAP CHI SINH HOC Vol. 34, No. 4 ( 2013-01-22)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 34, No. 4 ( 2013-01-22)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v34n4.2683
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2013
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Cloning, expression and purification of Leptospira LigB antigen in Escherichia coli
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 17, No. 3 ( 2020-11-28), p. 569-575
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 17, No. 3 ( 2020-11-28), p. 569-575
    Abstract: Leptospira is one of the most common zoonotic diseases in the tropics and subtropics. Humans are infected by exposure to Leptospira contained water or food sources. Leptospirosis usually breaks out after the flood and causes several consequences for people and economy. Leptospirosis disease, if not rapidly detected and treated promptly, it causes serious consequences such as acute hepatitis-kidneys, meningitis and bleeding, heart and nerve complications, and severe illness can lead to death. Therefore, quick and accurate detection of Leptospira pathogen plays a very important role in Leptospirosis disease treatment. Among antigens of Leptospira, a conserved domain of LigB antigen (Leptospiral immunoglobulin-like protein) was reported that is present in the most of pathogenic serovars of Leptospira, but not in the non-pathogenic Leptospira biflexa, thus this conserved domain was used for production of Leptospirosis detection kits as well as vaccine for Leptospirosis. In order to create a kit for Leptospirosis diagonostic, especially detect anti-Leptospira antibodies in Leptospira infected serum and plasma samples, about 1kb gene fragment encoding for conserved domain of LigB (about 36 kb in molecular weight) was used as the material for producing of LigB protein by DNA recombinant technology. In this study, we present the results for cloning, expressing a conserved domain of LigB antigen in E. coli cells and purifying protein by affinity chromatography collumn. The result indicates that recombinant LigB protein was successfully expressed in E. coli Rosetta 1 and purified by Hitrap chealating collumn. The LigB protein concentration after purification reached 60 mg/L medium with 98% purity.  This purified protein will be used as the materials for creating Leptospirosis kit.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/17/3/14364
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Identification of a mutation in a Vietnamese family with Emery-Dreifuss Muscular Dystrophy using whole exome sequencing
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Academia Journal of Biology Vol. 44, No. 1 ( 2022-03-30), p. 33-41
    Details
    In: Academia Journal of Biology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 44, No. 1 ( 2022-03-30), p. 33-41
    Abstract: Emery-Dreifuss muscular dystrophy (EDMD) is a degenerative neuromuscular disease associated with at least nine genes, including EMD, LMNA, FHL1, TMEM43, SUN1, SUN2, TTN, SYNE1, and SYNE2. Herein, we identified a heterozygous missense LMNA mutation (NM_170707.4: c.1357C 〉 T,p.R453W) in three members of a Vietnamese family using whole-exome sequencing (WES), in which the proband was an 11-year-old girl presenting humeroperoneal muscle weaknesses and generalized contracture. Her father and one other relative also exhibited multiple signs of muscular atrophy and contracture. Sanger sequencing in the extended family verified the causative nature of this mutation, establishing a confirmed diagnosis of autosomal dominant Emery-Dreifuss muscular dystrophy (EDMD2). The clinical presentations of each patient in this study are different from each other, demonstrating the intrafamilial phenotypic variability of this mutation. Early identification of the underlying genetic course of the disease by sequencing, combined with clinical findings provides solid evidence to diagnosis process, genetic counseling and management strategy.
    Type of Medium: Online Resource
    ISSN: 2615-9023 , 2615-9023
    URL: Article
    DOI: 10.15625/2615-9023/16742
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    OPTIMIZED EXTRACTION AND IMMUNOSTIMULATORY EFFECTS OF POLYSACCHARIDES FROM PSEUDERANTHEMUM PALATIFERUM (NEES) RADLK
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 16, No. 2 ( 2018-12-17), p. 327-335
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 2 ( 2018-12-17), p. 327-335
    Abstract: Pseuderanthemum palatiferum (Ness) Radlk is a tree native of Vietnam with various use in traditional medicine. It has been used for the treatment of many diseases including wound, colitis, stomach-aches, trauma, high blood pressure, nephritis, diarrhoea. However, the research on effective constituents from P. palatiferum has mainly been focused on small-molecular compounds. Some compounds: flavonoids, phytol, palmitic acid, ß-sitosterol, triterpenoid saponin, stigmasterol and salicylic acid have been detected in leaves. Polysaccharides have attracted great attentions for its benefits to human health. Polysaccharides from natural sources have diverse anti-inflammatory, anticoagulant and wound healing activities. In this study, we extracted and determined the polysaccharide content from the leaves of P. palatiferum plant. The polysaccharide content in P. palatiferum leaves was 8.2% (± 0.65) in dry weight. The appropriate polysaccharide extraction conditions were determined as: 25% ethanol, material/solvent ratio (1g/10ml), extracted temperature of 70°C, extraction time 12 hours. The polysaccharide composition was purified by TCA 10%, with a purity of 77.8% (± 1.19). The immunostimulatory activities of polysaccharide XH were also examined in cyclophosphamide (CY)-induced immunosuppressed mice. Mice treated with polysaccharide XH exhibited increased white blood cell counts (p 〈 0.05) compared with immunosuppressed mice. These results indicate that polysaccharide XH from P. palatiferum can enhance immune function in CY-induced immunosuppressed mice.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/16/2/13445
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Preparation of fusarium oxysporum-inhibiting chito-oligosaccharide by chitin/chitosan-hydrolyzing enzyme from actinomycetes
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 4 ( 2022-05-03), p. 765-770
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 765-770
    Abstract: Chito-oligosaccharide (COS), one of remakable derivatives of chitosan, has almost chitosan’s biological function but better activities in antibacteria, antifungi and plant elicitor… Producing COS by biological enzymes shows many advantages in comparision with physical and chemical methods. In our study, COS was prepared using chitin/chitosan-hydrolyzing enzyme from actinomycetes. By analysis of 16S rDNA sequence, the enzyme producer VTCC 940003 belonged to Streptomyces macrosporeus. The strain’s ability to synthesize the enzyme having chitin/chitosan-hydrolyzing activity was first detected by agar plate method and then its activity equivalent to chitinase activity was determined using dinitrosalicylic acid (DNS). The enzyme activity in the strain culture was 24 ± 0.05 U/ml. The COS was prepared by using the enzyme from S. macrosporeus VTCC 940003 to hydrolyze chitosan. The degree of polymerization (DP) of the obtained COS was mainly DP3-DP5. At the concentration of 0,3‰ the COS inhibited 98% of spore germination of Fusarium oxysporum after 24 hours and this activity remained after 48 hours.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15633
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...