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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (4)
  • 1
    Online Resource
    Online Resource
    Research and development of genetically engineered soybean using insect-resistance genes derived from Bacillus thuringiensis
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 1 ( 2020-07-11), p. 1-21
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 1 ( 2020-07-11), p. 1-21
    Abstract: Soybean (Glycine max) is one of the crops which have high economic value and serve for food, feed and process of many countries around the world. However, there are many factors affecting the productivity of soybean, of which insect pests and diseases are the most harmful agents. Therefore, an application of biotechnology to transfer insect resistance genes derived from a species of bacteria Bacillus thuringiensis can contribute to increase soybean yield and significantly reducing pesticide use. Currently, there are many insecticidal proteins detected from B. thuringiensis such as Cry, Cyt and Vip with a broad and specific spectrum belonged to several orders Lepidoptera, Diptera, Coleoptera, Homopera, and Nematoda. Numerous studies have been implemented over the world to transfer genes encoding these proteins in combination or modified forms to increase their toxicity. Several events of genetically engineered soybean with stacked traits of insect resistance and herbicide tolerance are commercialized and approved to be cultured in many countries such as MON 87701 × MON 89788 or DAS-81419-2. In Vietnam, studies on genetically engineered soybean with insect resistance trait has been carried out. Moreover, the exploitation, screening and selection of high biodiversity and indigenous B. thuringiensis strains which habors specific genes capable of killing targeted insects and serve as materials for plant transformation are great scientific meaning and potential practical application. This will be an important source of materials to create many soybean cultivars with good ability of insect resistance in order to meet specific needs.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/1/15257
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Molecular markers for analysis of plant genetic diversity
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 4 ( 2021-05-24), p. 589-608
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 4 ( 2021-05-24), p. 589-608
    Abstract: Genetic diversity plays an important role in diversity conservation at multiple levels and supports to monitor and assess genetic variation. In plants, genetic diversity provides the ability to adapt and respond to environmental conditions that helps plants to survive through changing environments. Genetic diversity analyses based on molecular genetic markers are effective tools for conservation and reintroduction of rare and endangered species. In recent years, the development of various chemical and molecular techniques for studying genetic diversity has received great attention. While biochemical markers are primarily used in the diagnosis of pathogens, DNA markers have been developed and widely applied for identification of species and population based on the genotype of an organism that is more stable and not easily affected by the environmental factors. PCR-based molecular marker tools, such as restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSRs) are used for analysing the difference in the targeted DNA sequences. With the rapid and robust development of genomic sequencing technology it is now possible to obtain and analyse DNA sequences of the whole genome of studied organisms. However, each type of DNA markers has different principles, as well as the pros and cons of specificity. In this article, we review methods and point out DNA markers, which are considered as reliable and widely used tools for the detection of genetic variation. In addition, we present the application of DNA marker in analysing genetic diversity of wild, domestic and medicinal plants, as well as some perspectives on the future of DNA marker’s application in the analysis of genetic diversity.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/4/15326
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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  • 3
    Online Resource
    Online Resource
    Construction an expression vector and Agrobacterium tumefaciens strain carrying ZmbZIP72 gene isolated from maize
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 15, No. 2 ( 2018-04-20), p. 333-340
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 15, No. 2 ( 2018-04-20), p. 333-340
    Abstract: In plants, members of bZIP transcription factors are involved in various biological processes such as organ and tissue differentiation or vascular development. Moreover, the basic leucine zipper (bZIP) is one of the largest transcription factor families which play an important role in environmental stress responses. However, few bZIP genes are related to abiotic stress responses. Some studies have found that transgenic plants overexpressing the bZIP genes enhanced tolerance to abiotic stresses. Several of stress-related genes in maize have been identified and characterized. A ZmbZIP72 gene belonged to A group could be strongly induced by ABA and abiotic stresses. Some previous studies have shown that overexpression of the ZmbZIP72 gene resulted in improved drought and salt tolerance of a transgenic Arabidopsis. In this work, the ZmbZIP72 gene from drought-treated maize was isolated and sequenced with 894 bp in full-length of the coding region. Nucleotide comparison of the ZmbZIP72 gene and a GenBank sequence (accession number HQ328839) revealed that two changes were found at positions 486 (A to C) and 493 (C to T), respectively. Changes in predicted amino acid sequence were at positions 102 (Lys 〉 Asp) and 105 (Pro 〉 Ser). The newly isolated ZmbZIP72 gene was ligated with RD29A promoter and 35S terminator to create a RD29::ZmbZIP72::35S cassette in pRTL2 vector. Afterwards, this cassette was constructed into pCAMBIA1301 and the new recombinant pCAMBIA1301 vector carrying ZmbZIP72 construct has transformed into A. tumefaciens strain EHA105 which is material for plant transformation.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15/2/12350
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 4
    Online Resource
    Online Resource
    Application of DNA barcodes in identification of ginseng samples in the genus Panax L.
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 15, No. 1 ( 2018-04-20), p. 63-72
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 15, No. 1 ( 2018-04-20), p. 63-72
    Abstract: Identification of species based on DNA sequences (DNA barcodes) is an aid to a taxonomic classification using morphological characteristics. DNA barcoding uses standard short genomic regions that are universally present in target lineages and has sufficient sequence variation to identify species in the genus. A variety of loci has been suggested as DNA barcodes for plants, including genes and non-coding regions in the nuclear and plastid genomes such as psbA-trnH, matK, rbcL, and ITS. In this study, we evaluated five potential DNA barcodes including 18S, ITS, matK, psbA-trnH, and rbcL for their ability to distinguish between species across our samples in the genus Panax L. Multiple alignments with 41 GenBank sequences selected from 9 species showed that the 18S region had the highest level of average similarity (99.87 %), followed by rbcL, matK, psbA-trnH, and ITS regions with lower levels of average similarities (99.27 %, 98.66 %, 96.82 % and 96.50 %, respectively). The phylogenetic trees showed that four of eleven ginseng samples were Panax vietnamensis Ha et Grushv., and three samples were Panax stipuleanatus with bootstrap values of 100 %. However, sequences of all five screened loci in the last four samples, which were considered as Panax bipinnatifidus based on morphological characteristics, were highly similar to Panax stipuleanatus with the level of sequence similarity reached 99.81 % - 100 %. In addition, our results showed that of five investigated DNA regions, ITS, and psbA-trnH were the most promising barcodes that could identify P. vietnamensis Ha et Grushv. and P. stipuleanatus species within the genus Panax L.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15/1/12321
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
    Location Call Number Limitation Availability
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    Online Resource
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