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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (11)
Materialart
Verlag/Herausgeber
  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (11)
Sprache
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  • 1
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 4 ( 2021-05-24), p. 679-691
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 4 ( 2021-05-24), p. 679-691
    Kurzfassung: Artichoke (Cynara scolymus L.), a high economic value crop, was brought to Vietnam by the French in the late 19th century. The artichoke was mainly planted in Lam Dong, Lao Cai, Vinh Phuc provinces, etc. At present, the disease situation of Artichoke plants and the lack of disease-free seedlings result in insufficient source of Artichoke for producers. Artichoke plants are mainly vegetative propagation and pathogens easily transferred from mother to daughter plants. Therefore, low propagation rate and fungal infection are two main factors hindering the expansion and development of Artichoke cultivation (in Lam Dong). Therefore, studying and evaluating the situation of fungi and viruses as well as establishing the in vitro propagation procedures in order to produce high number of disease-free seedlings are urgent for the current Artichoke shortage. In this study, samples of purple and white Artichoke varieties, which suspected fungal manifestations, were collected to assessment of fungi and viruses in artichoke. In addition, in vitro propagation by cultivating apical meristem was applied to produce disease-free seedlings. The recorded results showed that, 19 strains of mold were identified on purple and white Artichoke belonged to nine genera including Mucor (M. sp., M. circinelloides, M. fragilis, M. irregularis, and M. racemosus), Alternaria (Alternaria sp., A. alterinata, A. gaisen, A. tenuissima, and A. tillandsiae), Fusarium (F. acuminatum and F. solani), Cylindrobasidium (Cylindrobasidium sp1 and Cylindrobasidium sp2), Actinomucor elegans, Curvalaria clavata, Plectosphaerella oligotrophica, Phoma herbarum, Rhizomucor variabillis; meanwhile, the Tomato mosaic virus (ToMV) was isolated only purple Artichoke. Shoot explants obtained from apical meristem culture were completely disease-free and used for micropropagation at the next stage.
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2021
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2023
    In:  Vietnam Journal of Science and Technology Vol. 61, No. 2 ( 2023-04-17)
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 61, No. 2 ( 2023-04-17)
    Kurzfassung: Colletotrichum species cause anthracnose in various tropical crops in both cultivation and postharvest periods. The current synthetic fungicides to treat anthracnose sometime show toxic effects for farmers, residues on foods, and environmental impacts. In this work, turmeric extract containing curcuminoids was used as an active ingredient and formulated into a nanoemulsion to control anthracnose in red pepper and tomato fruits. The nanoparticles containing turmeric extract (25 %) were successfully prepared by encapsulation using Tween-80 and PEG 400 as emulsifiers. Out of the three main curcuminoids in the turmeric extract, BDMC was determined as the most abundant constituent via HPLC analysis. Turmeric nanoparticles showed an average particle size of 203 nm, polydispersity index (PI) of 0.322, and zeta potential of -92.7 mV.  In the in vitro antifungal bioassay, this nanoformulation significantly inhibited the mycelial growth of Colletotrichum gloeosporioides and Colletotrichum orbiculare in a dose-dependent manner. Red pepper anthracnose was also consistently suppressed by turmeric nanoparticles but the tomato anthracnose was resistant to this formulation at 10 mg/mL in vivo. The study results proved the effectiveness of turmeric nanoparticles to control red pepper anthracnose in postharvest preservation and suggested developing the innovative nanoformulation as a green fungicide.
    Materialart: Online-Ressource
    ISSN: 2815-5874 , 2525-2518
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2023
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 1 ( 2021-07-18), p. 129-145
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 1 ( 2021-07-18), p. 129-145
    Kurzfassung: Artichoke (Cynara scolymus L.), a medicinal plant with high economic value, contains high levels of phenolic compounds; especially cynarine, which plays an important role in preventing cancer, cardiovascular disease, osteoporosis, diabetes and neurodegeneration, etc. Currently, Artichoke micropropagation has achieved some success; however, the rooting efficiency and plantlet quality are still limited. In this study, improving the quality of Artichoke plantlet related to the shoot quality and suitable substrates in in vitro rooting stage was studied on “Violetta” Artichoke (VA) and “Green Globe” Artichoke (GA). The results showed that shoots (1.5 cm) cultured on MS medium supplemented 0.5 mg/L KIN were most suitable to shoot multiplication of VA with the number of shoots/explant (3.67 shoots), number of shoots ≥ 2 cm (3 shoots); while, 1.0 mg/L BA was suitable to shoot multiplication of GA (5.33 shoots; 5.00 shoots; respectively) after 4 weeks of culture. Besides, the in vitro rooting was improved using 8 g/L commercial agar for VA; meanwwhile, 3 g/L gelrite for GA. In addition, the nylon bag culture system (120 mm × 250 mm) has potential in plantlet production (15 plants/bag) and can be applied for large scale micropropagation. In addition, VA and GA plantlets derived from in vitro culture gave the good acclimatization, growth and development after 8, 12 and 20 weeks cultivating at the green house conditions.
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2021
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 2 ( 2021-08-02), p. 337-347
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 2 ( 2021-08-02), p. 337-347
    Kurzfassung: In vitro culture establishment is one of the most important stages in micropropagation. The disinfectant effectiveness depends on the type of surface disinfectant, concentration and the time treatment. In this initial study, silver nanoparticles (AgNPs) were used as a disinfectant for petioles, flower stalks and stems of Begonia tuberous. In addition, thin cell layer culture (TCL) technique has been applied for the purpose of somatic embryogenesis. The results showed that AgNPs were effective in eliminating infectious microorganisms on B. tuberous explants; which were identified included 4 species of fungi (Fusarium sp., Aspergillus aculeatus, Trichoderma sp. and Penicillium sp.) and 1 species of bacteria (Pseudomonas sp.). At concentrations of 200 ppm and 300 ppm, AgNPs were not only effective in disinfection but also increased the induction rate of somatic embryogenesis in flower stalk TCL explants (approximately 40.00%); a similar effect was observed in stem TCL explants at the same concentration. Meanwhile, for petiole TCL explants, the induction rate of somatic embryogenesis was optimal when using AgNPs at a concentration of 100 - 300 ppm to disinfected the explant. In contrast, at high (400 ppm) or low (50 ppm) concentrations of AgNPs did not play a disinfecting role and stimulated somatic embryogenesis. In addition, explants derived from AgNPs sterilization did not show any abnormalities in somatic embryogenesis with shapes such as globular, heart, torpedo, and cotyledon. AgNPs showed double efficacy in sterilization of explants and improved efficiency of somatic embryogenesis from TCL petioles, flower stalks and stems explants; thus increasing the efficiency micropropagation of B. tuberous.
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2021
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 2 ( 2020-11-03), p. 307-319
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 2 ( 2020-11-03), p. 307-319
    Kurzfassung: In plant tissue culture, iron nanoparticles (FeNPs) was one of the first types of nano to be used in plants. Previous reports have identified the effect of FeNPs on many different plant species. In this study, FeNPs was used to replace Fe-EDTA in MS (Murashige, Skoog, 1962) medium to assess their effects on growth, chlorophyll (a, b and a+b) accumulation, antioxidant activity of ascorbate peroxidase (APX) and superoxide dismutase (SOD) enzymes, and acclimatization in greenhouse conditions in different culture systems (in vitro solid, in vitro hydroponic and microponic culture). The obtained results show that FeNPs added to MS medium was higher growth, chlorophyll (a, b and a+b) content, antioxidant activity of SOD and APX enzymes than Fe-EDTA in MS medium as control treatment. The effect of FeNPs are differences between culture systems. In vitro solid and microponic culture systems, the optimal concentration is 75 mM FeNPs and in vitro hydroponic culture system is 100 mM FeNPs. The optimal activity of the antioxidant enzyme SOD (35.04 U.mg−1 prot) obtained in the roots of cultured plants in microponic culture system; meanwhile, the optimal activity of the antioxidant enzyme APX (2.11 μmol.min−1.mg−1 prot) obtained in leaves cultivated in solid culture system. The plantlets derived from MS medium added FeNPs were transfered into greenhouse conditions, the microponic cultivated plants supplemented with FeNPs at a concentration of 100 mM gave the highest survival rate (94.67%). The results of this study showed that FeNPs can replace Fe-EDTA salt in MS medium, and iron deficiency in culture media will reduce chlorophyll content.
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2020
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 4 ( 2022-05-03), p. 717-724
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 717-724
    Kurzfassung: Saintpaulia ionantha Wendl. is a small flower, with a variety of colors, which are commonly grown indoors for decorative purposes. Currently, micropropagation of S. ionantha has been achieved certain results; however, the shoot quality as well as number of shoots regeneration was not high. In this study, the effect of LEDs system on the shoot multiplication and shoot quality of S. ionantha was conducted. The leaves were cut into square patterns without the main veins (1 cm × 1 cm), and the petiole (3 mm in diameter) was cut with a thick layer (2 mm) of longitudinal cell (lTCL), which were used as the initial materials. The explants were placed under LEDs conditions; and white fluorescent tube lamp (FL) was used as the control for shoot regeneration and rooting. The results found that the quality of light affects the regeneration, growth and development of S. ionantha shoots. The LED condition with a combination of 7R + 3B are optimal for high-frequency of shoot regeneration with the regeneration rate: 67.25%, and number of shoots: 9.33 shoots/explant from the leaves without the main veins. The higher shoot multiplication (number of shoots: 10.00 shoots/explant, fresh weight: 150.07 mg, dry weight: 98.64 mg) and rooting rate of plantlet (fresh weight: 2.17 g; dry weight: 0.09 g; leaf number: 20.33 leaves/plantlet) were also obtained under 7R + 3B condition.
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2022
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 16, No. 1 ( 2018-12-17), p. 87-97
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 16, No. 1 ( 2018-12-17), p. 87-97
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2018
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2012
    In:  TAP CHI SINH HOC Vol. 34, No. 3 ( 2012-10-03)
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 34, No. 3 ( 2012-10-03)
    Materialart: Online-Ressource
    ISSN: 0866-7160 , 0866-7160
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2012
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2012
    In:  TAP CHI SINH HOC Vol. 34, No. 3se ( 2012-08-06)
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 34, No. 3se ( 2012-08-06)
    Materialart: Online-Ressource
    ISSN: 0866-7160 , 0866-7160
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2012
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
    Online-Ressource
    Online-Ressource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 1 ( 2016-03-30), p. 75-86
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 1 ( 2016-03-30), p. 75-86
    Kurzfassung: Ngoc Linh ginseng also known as Vietnamese ginseng (Panax Vietnamensis Ha et Grushv.) is a perennial medicinal plant. This plant is extremely rare and belongs to the Araliaceae family. Scientists are focusing on studies of Ngoc Linh ginseng nowadays. In this research, the effects of 2,4-dichlorophenoxyacetic acid (2,4-D), a-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 6-benzylaminopurine (BA), Kinetin (KIN), mineral salt formulations and cultural conditions, pH, sucrose concentration, medium volume on cell suspension culture of Panax vietnamensis Ha et Grushv. were investigated. In addition, growth curves and the effect of several plant grow regulators including a-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) on the regeneration of Ngoc Linh ginseng‘s cell suspension were also presented in this study. After 28 days in culture, the results showed that the best growth of a cell suspension of Ngoc Linh ginseng were obtained on ½MS liquid medium supplemented with 1.5 mg/l NAA, 50 g/l sucrose and the most suitable pH was 6.3. The acceptable medium volume for cell suspension growth was 30 ml. The growth curve of Ngoc Linh ginseng’s cell suspension showed that it should be subcultured at the beginning of the stationary phase approximately the 14th-16th day of culture. Ngoc Linh ginseng’s cell suspension exhibited the strongest growth at this time. When Ngoc Linh ginseng’s cell suspension was transferred to fresh medium, somatic embryos were formed in MS medium supplemented with 3.0 mg/l NAA after 30 days culture. The results shown that the potential regeneration of cell suspension of Ngoc Linh ginseng is very high.
    Materialart: Online-Ressource
    ISSN: 1811-4989 , 1811-4989
    Sprache: Unbekannt
    Verlag: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publikationsdatum: 2016
    Standort Signatur Einschränkungen Verfügbarkeit
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