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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (63)
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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (63)
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  • 1
    Online Resource
    Online Resource
    EFFECTS OF PROCESS PARAMETERS ON THE EXTRACTION EFFICIENCY AND CHEMICAL CHARACTERISTICS OF TEA SEED OIL FROM “TRUNGDU” TEA VARIETY
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2019
    In:  Vietnam Journal of Science and Technology Vol. 57, No. 3B ( 2019-11-12), p. 75-
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 57, No. 3B ( 2019-11-12), p. 75-
    Abstract: This research was aimed at investigating the effects of some extraction process parameters on the extraction efficiency and some chemical characteristics of tea seed oil from “Trungdu” tea variety, one of the high-quality vegetable oils, similar to olive oil with high portion of unsaturated fatty acids, especially essential linoleic acid and low content of saturated fat. This work reports for the first time the effects of process parameters using solvent extraction method on the efficiency and chemical characteristics of “Trungdu” tea seed oil. The study parameters were particle size (0.25-2.00 mm), material/solvent ratio (1/12-1/6), temperature (25-55oC), time (5-11 h), speed of solvent movement (0-250 r/m) and extraction cycle (1-3 times). The responses such as extraction efficiency, chemical characteristics (acid, peroxide, iodine and saponification values) were determined. The results indicated that the extraction efficiency was affected by all designed parameters. In addition, the temperature, particle size and extraction time had the most significant effect on the iodine value and acid value. The extraction temperature and the speed of solvent movement had pronounced effects on all the acid value, peroxide value, iodine value and saponification value of tea seed oil. However, ratio of material/solvent and extraction cycle did not appreciably effect on chemical characteristics of tea seed oil. Furthermore, the optimal extraction efficiency related to higher quality properties had been achieved in the range of 35 to 45oC, 7 to 9 hours, with a particle size of 0.5 mm, material/content ratio 1/8 to 1/12, movement speed of solvent 200 to 250 r/m and extraction cycle 1 or 2 times. Therefore, the results from this work will be useful for developing an optimal procedure for obtaining tea seed oil.  In addition, tea seed oil with the main acid component is oleic acid and linoleic acid, is considered a high quality oil.
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/57/3B/14390
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2019
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    Online Resource
  • 2
    Online Resource
    Online Resource
    INVESTIGATING THE ANTI-INFLAMMATORY ACTIVITY OF AN ETHANOLIC EXTRACT FROM ARTOCARPUS TONKINENSIS LEAVES USING A COLLAGEN ANTIBODY-INDUCED ARTHRITIC MOUSE MODEL
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Science and Technology Vol. 56, No. 3 ( 2018-06-11), p. 286-
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 56, No. 3 ( 2018-06-11), p. 286-
    Abstract: The obtained results here demonstrate that the 70% ethanolic leaf extract of A. tonkinensis (AT2), traditionally used in Vietnamese folk medicine for treating arthritic symtoms, has beneficial effects on pro-inflammatory cytokine inhibition and in an experimental arthritic mouse model. LPS-stimulated RAW 264.7 macrophages treated with AT2 showed a significant decrease in the production of IL-6 and TNFa at concentrations of 12.5, 25 and 50 µg/mL (P 〈 0.05), indicating its potential anti-inflammatory properties. Treatment of CAIA mice with AT2 also led to diminish the incidence of arthritis at doses of 200 and 300 mg/kg body weight.
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/56/3/11105
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Isolation, selection and evaluation of α-glucosidase inhibitory activity from endophytic Streptomyces sp. isolated from Citrus myrtifolia cultivar in Hoa Binh, Vietnam
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 20, No. 4 ( 2022-12-28), p. 693-704
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 20, No. 4 ( 2022-12-28), p. 693-704
    Abstract: Cao Phong orange (Citrus sinensis Lour) was grown in Cao Phong district, Hoa Binh province. Citrus sinensis is Vietnam’s most valued commercial fruit. In this study, 30 microorganim strains were isolated from the samples of root, stem, and leaf of Cao Phong orange. The ratio of endophytic actinomycetes was found in different parts of the plant: root, stem, and leaf was 37%, 40%, and 23%, respectively. The isolating HBC6-2 was selected because of its high output of α-glucosidase inhibitors from 6 different strains. Morphological, physiological, and biochemical characterization showed that HBC6-2 strain belongs to the Streptomyces sp. The 16S rRNA sequence of HBC6-2 indicated 99% identity to the corresponding sequence of Streptomyces costaricanus, and was registered on GenBank with the code MT 453944.1. Streptomyces costaricanus EBL.HB6 was able to produce melanin yelow pigment, and its aerial and substrate mycelia have brown and yellow-grey pigment on ISP2, ISP3, ISP4, and ISP5 cultivating medium, respectively. The optimal pH range was from 5-10 and temperature from 15-40°C and exhibited salt tolerance up to 3% and utilized the carbon sources such as fructose, xylose, arabinose, cellulose, and rhamnose. In the following investigation, Streptomyces costaricanus EBL.HB6 displayed the highest α-glucosidase inhibitory activity by 68.98% among Streptomyces sp. strains
    Type of Medium: Online Resource
    ISSN: 2815-5912 , 2815-5955
    URL: Article
    DOI: 10.15625/1811-4989/17172
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
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    Online Resource
  • 4
    Online Resource
    Online Resource
    Whole-genome sequencing and de novo assembly of a 2019 novel coronavirus (SARS-CoV-2) strain isolated in Vietnam
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 2 ( 2020-11-03), p. 197-208
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 2 ( 2020-11-03), p. 197-208
    Abstract: The pandemic COVID-19 caused by the virus SARS-CoV-2 has devastated countries worldwide, infecting more than 4.5 million people and leading to more than 300,000 deaths as of May 16th, 2020. Whole-genome sequencing (WGS) is an effective tool to monitor emerging strains and provide information for intervention, thus help to inform outbreak control decisions. Here, we reported the first effort to sequence and de novo assemble the whole genome of SARS-CoV-2 using PacBio’s SMRT sequencing technology in Vietnam. We also presented the annotation results and a brief analysis of the variants found in our SARS-CoV-2 strain, which was isolated from a Vietnamese patient. The sequencing was successfully completed and de novo assembled in less than 30 hours, resulting in one contig with no gap and a length of 29,766 bp. All detected variants as compared to the NCBI reference were highly accurate, as confirmed by Sanger sequencing. The results have shown the potential of long read sequencing to provide high quality WGS data to support public health responses and advance understanding of this and future pandemics.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/2/15082
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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    Online Resource
  • 5
    Online Resource
    Online Resource
    The resistant ability to weevils and the characteristics of VrPDF1 gene of some mungbean cultivars (Vigna radiata L.Wilczek)
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 1 ( 2016-03-30), p. 105-113
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 1 ( 2016-03-30), p. 105-113
    Abstract: Plant defensins play a role against the seed-feeding insects. Defensin associates with the center of α-amylase activity in the gut of weevils, thus inhibiting the digestion of starch by weevils. In this study, the resistance of eight mungbean cultivars to weevils was evaluated by the method of artificial weevils infection. The Tam TH cultivar had lowest index of susceptibility to weevils (634.63) and DX22 cultivar had highest index (1058.72), and the highest resistance to weevils was found in Tam TH and DX22 was found to have the lowest resistance. VrPDF1 genes isolated from mungbean cultivars are 356 bp in length with two exons interrupted by an intron. The coding region of the VrPDF1 gene is 228 bp in length, encoding 75 amino acids. The comparative results of the nucleotide sequence of cDNA between Tam TH  and DX22 showed that there was a difference in 13 nucleotides and comparison of amino acid sequences of the deduced protein indicated that there was a difference in 9 amino acids. Within the intron region of the VrPDF1 genes there was difference in 5 nucleotides. The genetic distance based on nucleotide sequences of the coding region of VrPDF1 gene of DX22 and seven other mungbean cultivars is 6.2% and based on the amino acid sequence deduced is 7.7%. The coding region of the VrPDF1 gene of DX22 was used to create a transformation vector aimed at creating weevil-resistant transgenic mungbean.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/14/1/9300
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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    Online Resource
  • 6
    Online Resource
    Online Resource
    Promising activity against NTERA-2 cancer stem cells of malloapelta B compound isolated from the Bum-bup plant in Vietnam
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2020
    In:  Vietnam Journal of Biotechnology Vol. 18, No. 1 ( 2020-07-11), p. 117-125
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 18, No. 1 ( 2020-07-11), p. 117-125
    Abstract: Recent studies have revealed that cancer stem cells (CSCs) drive tumor growth, metastasis, therapy resistant and recurrence. Therefore, CSCs are considered as new targets for screening of more effective drugs. The compound malloapelta B, which was isolated from the Bum-bup plant (Mallotus apelta) (Lour.) Muel.-Arg, Euphorbiaceae) in Vietnam, presented very potent and strong anticancer invitro, especially against the activation ofNF-kB (nuclear factor- kappa B). In our research, malloapelta B inhibited the growth of NTERA-2 line, a CSC line, at the value of IC50 = 12.71 ± 0.76 µM. In this study, malloapelta B also had effects on cell cycle of NTERA-2 by significantly reducing the cell number at G0/G1 phase (37.48%), while raising this number of phase G2/M to 31.12% in comparison with those of control (56.81% and 18.6%, respectively). Besides, clonogenic and tumorspheroidal formations are acknowledged as typically renewable characteristics of CSCs. Then, in our studies, malloapelta B significantly prevented NTERA-2 to form clonogens and reduced tumorspheres’ sizes at treatments with 20 and 100 µg/mL. However, malloapelta B did not exhibit any obvious effects on the expression level of CD44+/CD24+, the two typical cell surface markers of CSCs, at 2.5 and 5 µM treatments.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/18/1/15270
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2020
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  • 7
    Online Resource
    Online Resource
    Development of an in vitro hairy root induction system in different soybean cultivars for gene expression and genome editing studies
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 3 ( 2021-10-13), p. 459-470
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 3 ( 2021-10-13), p. 459-470
    Abstract: Hairy root induction system has been widely applied for studies of gene function, gene expression, and genome editing in numerous plant species. In this study, we developed and evaluated the performance of an in vitro hairy root induction system via Agrobacterium rhizogenes on several Vietnamese and international soybean cultivars. The efficacy of in vitro hairy root induction and of transformation using this system was varied and depended on soybean cultivars as well as transgenic constructs. The hairy root induction frequency of different soybean cultivars ranged from 61.67% to 100% after 5 days on culture medium. From 43.8% to 79.8% of hairy roots transformed with GFP-expressing construct showed transgene expression, while that for the construct with the gus gene was from 38.07% to 72.33%. Among tested soybean cultivars, DT26 demonstrated the highest transformation and gene expression efficacy with both investigated vectors. This hairy root induction system was further utilized for targeted knockout mutagenesis via CRISPR/Cas9 of two genes which are G03 and G09 in the DT26 soybean cultivar. Successful mutagenesis in the regions of targeted genes was confirmed by shifted and multiple bands compared to which of non-transgenic hairy root in PAGE analysis. Sequencing results of targeted regions presented various nucleotide deletions ranging between -3 bp and -25 bp in size in both two genes of interest. This study laid an important basis for future gene function studies and targeted gene editing investigations on soybean plants in Vietnam.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15421
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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  • 8
    Online Resource
    Online Resource
    Saponin production by cell culture techniques of Polyscias fruticosa L. Harms
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2015
    In:  TAP CHI SINH HOC Vol. 37, No. 1se ( 2015-04-25)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 37, No. 1se ( 2015-04-25)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v37n1se.6102
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2015
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  • 9
    Online Resource
    Online Resource
    Evaluation and application of Drosophila melanogaster model for anti-cancer herb screening
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2015
    In:  TAP CHI SINH HOC Vol. 37, No. 1se ( 2015-04-25)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 37, No. 1se ( 2015-04-25)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v37n1se.6117
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2015
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  • 10
    Online Resource
    Online Resource
    Bioactivity of some compounds isolated from the heartwood of Dalbergia oliveri Gamble ex Prain
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2014
    In:  TAP CHI SINH HOC Vol. 35, No. 4 ( 2014-07-28)
    Details
    In: TAP CHI SINH HOC, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 35, No. 4 ( 2014-07-28)
    Type of Medium: Online Resource
    ISSN: 0866-7160 , 0866-7160
    URL: Article
    DOI: 10.15625/0866-7160/v35n4.3772
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2014
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