In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 9 ( 2022-9-12), p. e1010338-
Abstract:
Actin cytoskeleton is essential for root hair formation. However, the underlying molecular mechanisms of actin dynamics in root hair formation in response to abiotic stress are largely undiscovered. Here, genetic analysis showed that actin-depolymerizing protein ADF7 and actin-bundling protein VILLIN1 (VLN1) were positively and negatively involved in root hair formation of Arabidopsis respectively. Moreover, RT-qPCR, GUS staining, western blotting, and genetic analysis revealed that ADF7 played an important role in inhibiting the expression and function of VLN1 during root hair formation. Filament actin (F-actin) dynamics observation and actin pharmacological experiments indicated that ADF7-inhibited-VLN1 pathway led to the decline of F-actin bundling and thick bundle formation, as well as the increase of F-actin depolymerization and turnover to promote root hair formation. Furthermore, the F-actin dynamics mediated by ADF7-inhibited-VLN1 pathway was associated with the reactive oxygen species (ROS) accumulation in root hair formation. Finally, ADF7-inhibited-VLN1 pathway was critical for osmotic stress-induced root hair formation. Our work demonstrates that ADF7 inhibits VLN1 to regulate F-actin dynamics in root hair formation in response to osmotic stress, providing the novel evidence on the F-actin dynamics and their molecular mechanisms in root hair formation and in abiotic stress.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010338
DOI:
10.1371/journal.pgen.1010338.g001
DOI:
10.1371/journal.pgen.1010338.g002
DOI:
10.1371/journal.pgen.1010338.g003
DOI:
10.1371/journal.pgen.1010338.g004
DOI:
10.1371/journal.pgen.1010338.g005
DOI:
10.1371/journal.pgen.1010338.g006
DOI:
10.1371/journal.pgen.1010338.g007
DOI:
10.1371/journal.pgen.1010338.g008
DOI:
10.1371/journal.pgen.1010338.s001
DOI:
10.1371/journal.pgen.1010338.s002
DOI:
10.1371/journal.pgen.1010338.s003
DOI:
10.1371/journal.pgen.1010338.s004
DOI:
10.1371/journal.pgen.1010338.s005
DOI:
10.1371/journal.pgen.1010338.s006
DOI:
10.1371/journal.pgen.1010338.s007
DOI:
10.1371/journal.pgen.1010338.s008
DOI:
10.1371/journal.pgen.1010338.s009
DOI:
10.1371/journal.pgen.1010338.s010
DOI:
10.1371/journal.pgen.1010338.s011
DOI:
10.1371/journal.pgen.1010338.s012
DOI:
10.1371/journal.pgen.1010338.s013
DOI:
10.1371/journal.pgen.1010338.s014
DOI:
10.1371/journal.pgen.1010338.s015
DOI:
10.1371/journal.pgen.1010338.s016
DOI:
10.1371/journal.pgen.1010338.s017
DOI:
10.1371/journal.pgen.1010338.s018
DOI:
10.1371/journal.pgen.1010338.s019
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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