In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 7 ( 2023-7-11), p. e0279018-
Abstract:
Lung cancer is the second most commonly diagnosed cancer and the leading cause of cancer-related death. Malignant pleural effusion (MPE) is a special microenvironment for lung cancer metastasis. Alternative splicing, which is regulated by splicing factors, affects the expression of most genes and influences carcinogenesis and metastasis. Methods mRNA-seq data and alternative splicing events in lung adenocarcinoma (LUAD) were obtained from The Cancer Genome Atlas (TCGA). A risk model was generated by Cox regression analyses and LASSO regression. Cell isolation and flow cytometry were used to identify B cells. Results We systematically analyzed the splicing factors, alternative splicing events, clinical characteristics, and immunologic features of LUAD in the TCGA cohort. A risk signature based on 23 alternative splicing events was established and identified as an independent prognosis factor in LUAD. Among all patients, the risk signature showed a better prognostic value in metastatic patients. By single-sample gene set enrichment analysis, we found that among tumor-infiltrating lymphocytes, B cells were most significantly correlated to the risk score. Furthermore, we investigated the classification and function of B cells in MPE, a metastatic microenvironment of LUAD, and found that regulatory B cells might participate in the regulation of the immune microenvironment of MPE through antigen presentation and promotion of regulatory T cell differentiation. Conclusions We evaluated the prognostic value of alternative splicing events in LUAD and metastatic LUAD. We found that regulatory B cells had the function of antigen presentation, inhibited naïve T cells from differentiating into Th1 cells, and promoted Treg differentiation in LUAD patients with MPE.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0279018
DOI:
10.1371/journal.pone.0279018.g001
DOI:
10.1371/journal.pone.0279018.g002
DOI:
10.1371/journal.pone.0279018.g003
DOI:
10.1371/journal.pone.0279018.g004
DOI:
10.1371/journal.pone.0279018.g005
DOI:
10.1371/journal.pone.0279018.g006
DOI:
10.1371/journal.pone.0279018.t001
DOI:
10.1371/journal.pone.0279018.s001
DOI:
10.1371/journal.pone.0279018.s002
DOI:
10.1371/journal.pone.0279018.s003
DOI:
10.1371/journal.pone.0279018.s004
DOI:
10.1371/journal.pone.0279018.s005
DOI:
10.1371/journal.pone.0279018.s006
DOI:
10.1371/journal.pone.0279018.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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