In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2021-7-12), p. e1009680-
Abstract:
The evolution of insecticide resistance represents a global constraint to agricultural production. Because of the extreme genetic diversity found in insects and the large numbers of genes involved in insecticide detoxification, better tools are needed to quickly identify and validate the involvement of putative resistance genes for improved monitoring, management, and countering of field-evolved insecticide resistance. The avermectins, emamectin benzoate (EB) and abamectin are relatively new pesticides with reduced environmental risk that target a wide number of insect pests, including the beet armyworm, Spodoptera exigua , an important global pest of many crops. Unfortunately, field resistance to avermectins recently evolved in the beet armyworm, threatening the sustainable use of this class of insecticides. Here, we report a high-quality chromosome-level assembly of the beet armyworm genome and use bulked segregant analysis (BSA) to identify the locus of avermectin resistance, which mapped on 15–16 Mbp of chromosome 17. Knockout of the CYP9A186 gene that maps within this region by CRISPR/Cas9 gene editing fully restored EB susceptibility, implicating this gene in avermectin resistance. Heterologous expression and in vitro functional assays further confirm that a natural substitution (F116V) found in the substrate recognition site 1 (SRS1) of the CYP9A186 protein results in enhanced metabolism of EB and abamectin. Hence, the combined approach of coupling gene editing with BSA allows for the rapid identification of metabolic resistance genes responsible for insecticide resistance, which is critical for effective monitoring and adaptive management of insecticide resistance.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1009680
DOI:
10.1371/journal.pgen.1009680.g001
DOI:
10.1371/journal.pgen.1009680.g002
DOI:
10.1371/journal.pgen.1009680.g003
DOI:
10.1371/journal.pgen.1009680.g004
DOI:
10.1371/journal.pgen.1009680.g005
DOI:
10.1371/journal.pgen.1009680.t001
DOI:
10.1371/journal.pgen.1009680.t002
DOI:
10.1371/journal.pgen.1009680.t003
DOI:
10.1371/journal.pgen.1009680.s001
DOI:
10.1371/journal.pgen.1009680.s002
DOI:
10.1371/journal.pgen.1009680.s003
DOI:
10.1371/journal.pgen.1009680.s004
DOI:
10.1371/journal.pgen.1009680.s005
DOI:
10.1371/journal.pgen.1009680.s006
DOI:
10.1371/journal.pgen.1009680.s007
DOI:
10.1371/journal.pgen.1009680.s008
DOI:
10.1371/journal.pgen.1009680.s009
DOI:
10.1371/journal.pgen.1009680.s010
DOI:
10.1371/journal.pgen.1009680.s011
DOI:
10.1371/journal.pgen.1009680.s012
DOI:
10.1371/journal.pgen.1009680.s013
DOI:
10.1371/journal.pgen.1009680.s014
DOI:
10.1371/journal.pgen.1009680.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2186725-2
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