In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 10 ( 2022-10-31), p. e0010881-
Abstract:
Pyruvate kinase widely exists in many parasites and plays an important role in the energy production for the parasites. Pyruvate kinase might be a potential drug target for killing the parasites. The aim of the present study was to evaluate the biological characteristics and roles of T . spiralis pyruvate kinase M (TsPKM) in sugar metabolism, larval molting and development of T . spiralis . Methodology/Principal findings TsPKM has two functional domains of pyruvate kinase and the tertiary structure of TsPKM is tetramer which has the enzyme active site constituted by 8 amino-acid residues (Arg71, Asn73, Asp110, Phe241, Lys267, Glu269, Asp293 and Thr325). Recombinant TsPKM (rTsPKM) was expressed and purified. The rTsPKM had good immunogenicity. RT-PCR and Western blot showed that TsPKM was transcribed and expressed at various developmental stages in T . spiralis lifecycle. Immunofluorescence test showed that TsPKM was principally located in the cuticle, muscle, stichosome, intestine and the intrauterine embryos of female adults. rTsPKM catalyzed the reaction of phosphoenolpyruvate (PEP) and adenosine diphosphate (ADP) to produce pyruvic acid and adenosine triphosphate (ATP). TsPKM played an important role in the metabolism and energy production of T . spiralis . After silencing of TsPKM gene by specific dsRNA-TsPKM2, protein expression and enzyme activity of TsPKM decreased by 50.91 and 26.06%, respectively. After treatment with RNAi, natural TsPKM enzyme activity, larval molting, sugar metabolism, growth and development of T . spiralis were significantly reduced. Conclusions TsPKM participates in the larval molting, sugar metabolism, growth and development of T . spiralis and it might be a candidate target of therapeutic drug of trichinellosis.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010881
DOI:
10.1371/journal.pntd.0010881.g001
DOI:
10.1371/journal.pntd.0010881.g002
DOI:
10.1371/journal.pntd.0010881.g003
DOI:
10.1371/journal.pntd.0010881.g004
DOI:
10.1371/journal.pntd.0010881.g005
DOI:
10.1371/journal.pntd.0010881.g006
DOI:
10.1371/journal.pntd.0010881.g007
DOI:
10.1371/journal.pntd.0010881.g008
DOI:
10.1371/journal.pntd.0010881.g009
DOI:
10.1371/journal.pntd.0010881.g010
DOI:
10.1371/journal.pntd.0010881.g011
DOI:
10.1371/journal.pntd.0010881.g012
DOI:
10.1371/journal.pntd.0010881.g013
DOI:
10.1371/journal.pntd.0010881.g014
DOI:
10.1371/journal.pntd.0010881.g015
DOI:
10.1371/journal.pntd.0010881.g016
DOI:
10.1371/journal.pntd.0010881.s001
DOI:
10.1371/journal.pntd.0010881.s002
DOI:
10.1371/journal.pntd.0010881.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
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