In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 9 ( 2022-9-22), p. e1010837-
Abstract:
The balance between immunity and reproduction is essential for many key physiological functions. We report that to maintain an optimal fertility, 20-hydroxyecdysone (20E) and the ecdysone receptor (EcR) downregulate the immune deficiency (IMD) pathway during the post blood meal phase (PBM) of the Aedes aegypti reproductive cycle. RNA interference-mediated depletion of EcR elicited an increased expression of the IMD pathway components, and these mosquitoes were more resistant to infection by Gram-negative bacteria. Moreover, 20E and EcR recruit Pirk-like, the mosquito ortholog of Drosophila melanogaster Pirk. CRISPR-Cas9 knockout of Pirk-like has shown that it represses the IMD pathway by interfering with IMD-mediated formation of amyloid aggregates. 20E and EcR disruption of the amyloid formation is pivotal for maintaining normal yolk protein production and fertility. Additionally, 20E and its receptor EcR directly induce Pirk-like to interfere with cRHIM-mediated formation of amyloid. Our study highlights the vital role of 20E in governing the trade-off between immunity and reproduction. Pirk-like might be a potential target for new methods to control mosquito reproduction and pathogen transmission.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010837
DOI:
10.1371/journal.ppat.1010837.g001
DOI:
10.1371/journal.ppat.1010837.g002
DOI:
10.1371/journal.ppat.1010837.g003
DOI:
10.1371/journal.ppat.1010837.g004
DOI:
10.1371/journal.ppat.1010837.g005
DOI:
10.1371/journal.ppat.1010837.g006
DOI:
10.1371/journal.ppat.1010837.g007
DOI:
10.1371/journal.ppat.1010837.s001
DOI:
10.1371/journal.ppat.1010837.s002
DOI:
10.1371/journal.ppat.1010837.s003
DOI:
10.1371/journal.ppat.1010837.s004
DOI:
10.1371/journal.ppat.1010837.s005
DOI:
10.1371/journal.ppat.1010837.s006
DOI:
10.1371/journal.ppat.1010837.s007
DOI:
10.1371/journal.ppat.1010837.s008
DOI:
10.1371/journal.ppat.1010837.s009
DOI:
10.1371/journal.ppat.1010837.s010
DOI:
10.1371/journal.ppat.1010837.s011
DOI:
10.1371/journal.ppat.1010837.s012
DOI:
10.1371/journal.ppat.1010837.r001
DOI:
10.1371/journal.ppat.1010837.r002
DOI:
10.1371/journal.ppat.1010837.r003
DOI:
10.1371/journal.ppat.1010837.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
Permalink