In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 10 ( 2021-10-11), p. e0258226-
Abstract:
We investigated the impact of human immunodeficiency virus (HIV) infection and anti-retroviral therapy (ART) on the gut microbiota of children. Design This cross-sectional study investigated the gut microbiota of children with and without HIV. Methods We collected fecal samples from 59 children with HIV (29 treated with ART [ART(+)] and 30 without ART [HIV(+)] ) and 20 children without HIV [HIV(–)] in Vietnam. We performed quantitative RT-PCR to detect 14 representative intestinal bacteria targeting 16S/23S rRNA molecules. We also collected the blood samples for immunological analyses. Results In spearman’s correlation analyses, no significant correlation between the number of dominant bacteria and age was found among children in the HIV(−) group. However, the number of sub-dominant bacteria, including Streptococcus , Enterococcus , and Enterobacteriaceae , positively correlated with age in the HIV(−) group, but not in the HIV(+) group. In the HIV(+) group, Clostridium coccoides group positively associated with the CD4 + cell count and its subsets. In the ART(+) group, Staphylococcus and C . perfringens positively correlated with CD4 + cells and their subsets and negatively with activated CD8 + cells. C . coccoides group and Bacteroides fragilis group were associated with regulatory T-cell counts. In multiple linear regression analyses, ART duration was independently associated with the number of C . perfringens , and Th17 cell count with the number of Staphylococcus in the ART(+) group. Conclusions HIV infection and ART may influence sub-dominant gut bacteria, directly or indirectly, in association with immune status in children with HIV.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0258226
DOI:
10.1371/journal.pone.0258226.g001
DOI:
10.1371/journal.pone.0258226.g002
DOI:
10.1371/journal.pone.0258226.g003
DOI:
10.1371/journal.pone.0258226.t001
DOI:
10.1371/journal.pone.0258226.t002
DOI:
10.1371/journal.pone.0258226.t003
DOI:
10.1371/journal.pone.0258226.s001
DOI:
10.1371/journal.pone.0258226.s002
DOI:
10.1371/journal.pone.0258226.s003
DOI:
10.1371/journal.pone.0258226.s004
DOI:
10.1371/journal.pone.0258226.s005
DOI:
10.1371/journal.pone.0258226.s006
DOI:
10.1371/journal.pone.0258226.s007
DOI:
10.1371/journal.pone.0258226.r001
DOI:
10.1371/journal.pone.0258226.r002
DOI:
10.1371/journal.pone.0258226.r003
DOI:
10.1371/journal.pone.0258226.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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