In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2021-7-15), p. e1009527-
Abstract:
Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in the cabbage butterfly Pieris rapae . Sinigrin, as a potent feeding stimulant, elicited activity in larval maxillary lateral sensilla styloconica, as well as in adult medial tarsal sensilla. Two P . rapae gustatory receptor genes PrapGr28 and PrapGr15 were identified with high expression in female tarsi, and the subsequent functional analyses showed that Xenopus oocytes only expressing PrapGr28 had specific responses to sinigrin; when ectopically expressed in Drosophila sugar sensing neurons, PrapGr28 conferred sinigrin sensitivity to these neurons. RNA interference experiments further showed that knockdown of PrapGr28 reduced the sensitivity of adult medial tarsal sensilla to sinigrin. Taken together, we conclude that PrapGr28 is a gustatory receptor tuned to sinigrin in P . rapae , which paves the way for revealing the molecular basis of the relationships between crucifer plants and their specialist insects.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1009527
DOI:
10.1371/journal.pgen.1009527.g001
DOI:
10.1371/journal.pgen.1009527.g002
DOI:
10.1371/journal.pgen.1009527.g003
DOI:
10.1371/journal.pgen.1009527.g004
DOI:
10.1371/journal.pgen.1009527.g005
DOI:
10.1371/journal.pgen.1009527.g006
DOI:
10.1371/journal.pgen.1009527.g007
DOI:
10.1371/journal.pgen.1009527.g008
DOI:
10.1371/journal.pgen.1009527.s001
DOI:
10.1371/journal.pgen.1009527.s002
DOI:
10.1371/journal.pgen.1009527.s003
DOI:
10.1371/journal.pgen.1009527.s004
DOI:
10.1371/journal.pgen.1009527.s005
DOI:
10.1371/journal.pgen.1009527.s006
DOI:
10.1371/journal.pgen.1009527.s007
DOI:
10.1371/journal.pgen.1009527.s008
DOI:
10.1371/journal.pgen.1009527.s009
DOI:
10.1371/journal.pgen.1009527.s010
DOI:
10.1371/journal.pgen.1009527.s011
DOI:
10.1371/journal.pgen.1009527.s012
DOI:
10.1371/journal.pgen.1009527.s013
DOI:
10.1371/journal.pgen.1009527.s014
DOI:
10.1371/journal.pgen.1009527.s015
DOI:
10.1371/journal.pgen.1009527.s016
DOI:
10.1371/journal.pgen.1009527.s017
DOI:
10.1371/journal.pgen.1009527.s018
DOI:
10.1371/journal.pgen.1009527.s019
DOI:
10.1371/journal.pgen.1009527.s020
DOI:
10.1371/journal.pgen.1009527.s021
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2186725-2
Permalink