In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 4 ( 2022-4-27), p. e0266144-
Abstract:
Postpartum uterine infection in dairy cows is commonly caused by pathogenic bacteria such as Escherichia coli ( E . coli ). Progesterone elicits immunosuppressive function within bovine endometrium, and has been suggested to be related to postpartum uterine infection. Endometrial stroma is exposed to bacteria due to the disruption of epithelium during parturition, but the effect and mechanism of progesterone on innate immune response of stromal cells has not been reported. This study evaluated the impact of progesterone on inflammatory response of primary endometrial stromal cells stimulated by lipopolysaccharide or heat-killed E . coli . Quantitative PCR analysis revealed that progesterone repressed mRNA induction of IL1B , IL6 , TNF , CXCL8 , NOS2 , and PTGS2 in stromal cells in response to lipopolysaccharide or E . coli challenge. Consistently, Western blot and immunofluorescence staining results showed that progesterone suppressed lipopolysaccharide- or E . coli -induced MAPK and NF-κB activations characterized with decreased phosphorylations of ERK1/2, JNK, P38, IκBα, and P65, and inhibition of P65 nuclear translocation. In unstimulated stromal cells, progesterone alone did not affect the mRNA transcription for IL6 , TNF , CXCL8 , NOS2 , and PTGS2 , and the signaling cascade of MAPK and NF-κB, but decreased IL1B mRNA expression. These results revealed that the anti-inflammatory effect of progesterone in lipopolysaccharide- or E . coli -challenged endometrial stromal cells was probably mediated through MAPK and NF-κB pathways.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0266144
DOI:
10.1371/journal.pone.0266144.g001
DOI:
10.1371/journal.pone.0266144.g002
DOI:
10.1371/journal.pone.0266144.g003
DOI:
10.1371/journal.pone.0266144.g004
DOI:
10.1371/journal.pone.0266144.g005
DOI:
10.1371/journal.pone.0266144.t001
DOI:
10.1371/journal.pone.0266144.s001
DOI:
10.1371/journal.pone.0266144.s002
DOI:
10.1371/journal.pone.0266144.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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