In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 12 ( 2020-12-22), p. e0244176-
Abstract:
Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-COV-2) is a significant threat to global health security. Till date, no completely effective drug or vaccine is available to cure COVID-19. Therefore, an effective vaccine against SARS-COV-2 is crucially needed. This study was conducted to design an effective multiepitope based vaccine (MEV) against SARS-COV-2. Seven highly antigenic proteins of SARS-COV-2 were selected as targets and different epitopes (B-cell and T-cell) were predicted. Highly antigenic and overlapping epitopes were shortlisted. Selected epitopes indicated significant interactions with the HLA-binding alleles and 99.93% coverage of the world’s population. Hence, 505 amino acids long MEV was designed by connecting 16 MHC class I and eleven MHC class II epitopes with suitable linkers and adjuvant. MEV construct was non-allergenic, antigenic, stable and flexible. Furthermore, molecular docking followed by molecular dynamics (MD) simulation analyses, demonstrated a stable and strong binding affinity of MEV with human pathogenic toll-like receptors (TLR), TLR3 and TLR8. Finally, MEV codons were optimized for its in silico cloning into Escherichia coli K-12 system, to ensure its increased expression. Designed MEV in present study could be a potential candidate for further vaccine production process against COVID-19. However, to ensure its safety and immunogenic profile, the proposed MEV needs to be experimentally validated.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0244176
DOI:
10.1371/journal.pone.0244176.g001
DOI:
10.1371/journal.pone.0244176.g002
DOI:
10.1371/journal.pone.0244176.g003
DOI:
10.1371/journal.pone.0244176.g004
DOI:
10.1371/journal.pone.0244176.g005
DOI:
10.1371/journal.pone.0244176.g006
DOI:
10.1371/journal.pone.0244176.g007
DOI:
10.1371/journal.pone.0244176.g008
DOI:
10.1371/journal.pone.0244176.g009
DOI:
10.1371/journal.pone.0244176.g010
DOI:
10.1371/journal.pone.0244176.g011
DOI:
10.1371/journal.pone.0244176.t001
DOI:
10.1371/journal.pone.0244176.t002
DOI:
10.1371/journal.pone.0244176.s001
DOI:
10.1371/journal.pone.0244176.s002
DOI:
10.1371/journal.pone.0244176.s003
DOI:
10.1371/journal.pone.0244176.s004
DOI:
10.1371/journal.pone.0244176.s005
DOI:
10.1371/journal.pone.0244176.s006
DOI:
10.1371/journal.pone.0244176.s007
DOI:
10.1371/journal.pone.0244176.s008
DOI:
10.1371/journal.pone.0244176.s009
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2267670-3
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