In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 6 ( 2022-6-29), p. e1010288-
Abstract:
Although evolutionary fates and expression patterns of duplicated genes have been extensively investigated, how duplicated genes co-regulate a biological process in polyploids remains largely unknown. Here, we identified two gsdf (gonadal somatic cell-derived factor) homeologous genes ( gsdf-A and gsdf-B ) in hexaploid gibel carp ( Carassius gibelio ), wherein each homeolog contained three highly conserved alleles. Interestingly, gsdf-A and gsdf-B transcription were mainly activated by dmrt1-A (dsx- and mab-3-related transcription factor 1) and dmrt1-B , respectively. Loss of either gsdf-A or gsdf-B alone resulted in partial male-to-female sex reversal and loss of both caused complete sex reversal, which could be rescued by a nonsteroidal aromatase inhibitor. Compensatory expression of gsdf-A and gsdf-B was observed in gsdf-B and gsdf-A mutants, respectively. Subsequently, we determined that in tissue culture cells, Gsdf-A and Gsdf-B both interacted with Ncoa5 (nuclear receptor coactivator 5) and blocked Ncoa5 interaction with Rora (retinoic acid-related orphan receptor-alpha) to repress Rora/Ncoa5-induced activation of cyp19a1a (cytochrome P450, family 19, subfamily A, polypeptide 1a). These findings illustrate that Gsdf-A and Gsdf-B can regulate male differentiation by inhibiting cyp19a1a transcription in hexaploid gibel carp and also reveal that Gsdf-A and Gsdf-B can interact with Ncoa5 to suppress cyp19a1a transcription in vitro . This study provides a typical case of cooperative mechanism of duplicated genes in polyploids and also sheds light on the conserved evolution of sex differentiation.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010288
DOI:
10.1371/journal.pgen.1010288.g001
DOI:
10.1371/journal.pgen.1010288.g002
DOI:
10.1371/journal.pgen.1010288.g003
DOI:
10.1371/journal.pgen.1010288.g004
DOI:
10.1371/journal.pgen.1010288.g005
DOI:
10.1371/journal.pgen.1010288.g006
DOI:
10.1371/journal.pgen.1010288.g007
DOI:
10.1371/journal.pgen.1010288.g008
DOI:
10.1371/journal.pgen.1010288.g009
DOI:
10.1371/journal.pgen.1010288.s001
DOI:
10.1371/journal.pgen.1010288.s002
DOI:
10.1371/journal.pgen.1010288.s003
DOI:
10.1371/journal.pgen.1010288.s004
DOI:
10.1371/journal.pgen.1010288.s005
DOI:
10.1371/journal.pgen.1010288.s006
DOI:
10.1371/journal.pgen.1010288.s007
DOI:
10.1371/journal.pgen.1010288.s008
DOI:
10.1371/journal.pgen.1010288.s009
DOI:
10.1371/journal.pgen.1010288.s010
DOI:
10.1371/journal.pgen.1010288.s011
DOI:
10.1371/journal.pgen.1010288.s012
DOI:
10.1371/journal.pgen.1010288.s013
DOI:
10.1371/journal.pgen.1010288.s014
DOI:
10.1371/journal.pgen.1010288.s015
DOI:
10.1371/journal.pgen.1010288.s016
DOI:
10.1371/journal.pgen.1010288.r001
DOI:
10.1371/journal.pgen.1010288.r002
DOI:
10.1371/journal.pgen.1010288.r003
DOI:
10.1371/journal.pgen.1010288.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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