In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2022-1-18), p. e0258283-
Abstract:
Staphylococcus epidermidis is a commensal bacterium in humans. To persist in the bacterial flora of the host, some bacteria produce antibacterial factors such as the antimicrobial peptides known as bacteriocins. In this study, we tried to isolate bacteriocin-producing S . epidermidis strains. Among 150 S . epidermidis isolates from the oral cavities of 287 volunteers, we detected two bacteriocin-producing strains, KSE56 and KSE650. Complete genome sequences of the two strains confirmed that they carried the epidermin-harboring plasmid pEpi56 and the nukacin IVK45-like-harboring plasmid pNuk650. The amino acid sequence of epidermin from KSE56 was identical to the previously reported sequence, but the epidermin synthesis-related genes were partially different. The prepeptide amino acid sequences of nukacin KSE650 and nukacin IVK45 showed one mismatch, but both mature peptides were entirely similar. pNuk650 was larger and had an additional seven ORFs compared to pIVK45. We then investigated the antibacterial activity of the two strains against several skin and oral bacteria and found their different activity patterns. In conclusion, we report the complete sequences of 2 plasmids coding for bacteriocins from S . epidermidis , which were partially different from those previously reported. Furthermore, this is the first report to show the complete sequence of an epidermin-carrying plasmid, pEpi56.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0258283
DOI:
10.1371/journal.pone.0258283.g001
DOI:
10.1371/journal.pone.0258283.g002
DOI:
10.1371/journal.pone.0258283.g003
DOI:
10.1371/journal.pone.0258283.g004
DOI:
10.1371/journal.pone.0258283.g005
DOI:
10.1371/journal.pone.0258283.g006
DOI:
10.1371/journal.pone.0258283.g007
DOI:
10.1371/journal.pone.0258283.t001
DOI:
10.1371/journal.pone.0258283.t002
DOI:
10.1371/journal.pone.0258283.t003
DOI:
10.1371/journal.pone.0258283.t004
DOI:
10.1371/journal.pone.0258283.t005
DOI:
10.1371/journal.pone.0258283.s001
DOI:
10.1371/journal.pone.0258283.s002
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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