In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 3 ( 2023-3-30), p. e0279118-
Abstract:
The Covid-19 associated mucormycosis (CAM) is an emerging disease affecting immunocompromised patients. Prevention of such infections using probiotics and their metabolites persist as effective therapeutic agents. Therefore, the present study emphasizes on assessment of their efficacy and safety. Samples from different sources like human milk, honey bee intestine, toddy, and dairy milk were collected, screened and characterized for potential probiotic lactic acid bacteria (LAB) and their metabolites to be used as effective antimicrobial agents to curtail CAM. Three isolates were selected based on probiotic properties and characterized as Lactobacillus pentosus BMOBR013, Lactobacillus pentosus BMOBR061 and Pediococcus acidilactici BMOBR041 by 16S rRNA sequencing and MALDI TOF-MS. The antimicrobial activity against standard bacterial pathogens showed ˃9 mm zone of inhibition. Furthermore, the antifungal activity of three isolates was tested against Aspergillus flavus MTCC 2788, Fusarium oxysporum , Candida albicans and Candida tropicalis where the results showed significant inhibition of each fungal strain. Further studies were carried out on lethal fungal pathogens like Rhizopus sp. and two Mucor sp. which are associated with post Covid-19 infection in immunosuppressed diabetic patients. Our studies on CAM inhibitory effect of LAB revealed the efficient inhibition against Rhizopus sp. and two Mucor sp. The cell free supernatants of three LAB showed varied inhibitory activity against these fungi. Following the antimicrobial activity, the antagonistic metabolite 3-Phenyllactic acid (PLA) in culture supernatant was quantified and characterized by HPLC and LC-MS using standard PLA (Sigma Aldrich). The isolate L . pentosus BMOBR013 produced highest PLA (0.441 g/L), followed by P . acidilactici BMOBR041 (0.294 g/L) and L . pentosus BMOBR061 (0.165 g/L). The minimum inhibitory concentration of HPLC eluted PLA on the Rhizopus sp. and two Mucor sp. was found to be 180 mg/ml which was further confirmed by inhibition of total mycelia under live cell imaging microscope.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0279118
DOI:
10.1371/journal.pone.0279118.g001
DOI:
10.1371/journal.pone.0279118.g002
DOI:
10.1371/journal.pone.0279118.g003
DOI:
10.1371/journal.pone.0279118.g004
DOI:
10.1371/journal.pone.0279118.g005
DOI:
10.1371/journal.pone.0279118.g006
DOI:
10.1371/journal.pone.0279118.g007
DOI:
10.1371/journal.pone.0279118.g008
DOI:
10.1371/journal.pone.0279118.g009
DOI:
10.1371/journal.pone.0279118.t001
DOI:
10.1371/journal.pone.0279118.t002
DOI:
10.1371/journal.pone.0279118.s001
DOI:
10.1371/journal.pone.0279118.r001
DOI:
10.1371/journal.pone.0279118.r002
DOI:
10.1371/journal.pone.0279118.r003
DOI:
10.1371/journal.pone.0279118.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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