In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 3 ( 2021-3-17), p. e0248614-
Abstract:
This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli , Klebsiella pneumoniae , Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae , ESBL or carbapenem-resistant E . coli , and K . pneumoniae , carbapenem-resistant A . baumannii complex, and P . aeruginosa . Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. Results Among blood isolates, A . baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E . cloacae complex showed higher resistance to carbapenems. A . baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K . pneumoniae isolates, bla TEM , bla SHV , and bla CTX were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E . coli isolates, bla TEM , bla SHV , and bla CTX were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was bla CTX-M-15 . Among Enterobacteriaceae , the most frequently detected carbapenemase-encoding gene was bla NDM-1 (81.5%), followed by bla OXA-232 (14.8%) and bla oxa-181 (7.4%), in A . baumannii was bla OXA-24 (76%) and in P . aeruginosa , was bla IMP (25.3%), followed by bla GES and bla VIM (13.1% each). Conclusion Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL bla CTX-M-15 exists in both E . coli and K . pneumoniae .
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0248614
DOI:
10.1371/journal.pone.0248614.t001
DOI:
10.1371/journal.pone.0248614.t002
DOI:
10.1371/journal.pone.0248614.t003
DOI:
10.1371/journal.pone.0248614.t004
DOI:
10.1371/journal.pone.0248614.t005
DOI:
10.1371/journal.pone.0248614.t006
DOI:
10.1371/journal.pone.0248614.t007
DOI:
10.1371/journal.pone.0248614.t008
DOI:
10.1371/journal.pone.0248614.s001
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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