In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-6), p. e0270499-
Abstract:
Burkholderia mallei is the etiological agent of glanders, a highly contagious and often fatal disease in equids. Due to the high genetic clonality of B . mallei , high-resolution typing assays are necessary to differentiate between individual strains. Here we report on the development and validation of a robust and reproducible core genome-based Multi Locus Sequence Typing Assay (cgMLST) for B . mallei , which is based on 3328 gene targets and enables high-resolution typing at the strain level. The assay was validated using a set of 120 B . mallei genomes from public databases and 23 newly sequenced outbreak strains from in-house strain collections. In this cgMLST analysis, strains from different geographic regions were clearly distinguished by at least 70 allele differences, allowing spatial clustering while closely related and epidemiologically related strains were separated by only zero to three alleles. Neither the different sequencing technologies nor the assembly strategies had an influence on the cgMLST results. The developed cgMLST is highly robust, reproducible and can be used for outbreak investigations, source tracking and molecular characterization of new B . mallei isolates.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0270499
DOI:
10.1371/journal.pone.0270499.g001
DOI:
10.1371/journal.pone.0270499.g002
DOI:
10.1371/journal.pone.0270499.g003
DOI:
10.1371/journal.pone.0270499.t001
DOI:
10.1371/journal.pone.0270499.s001
DOI:
10.1371/journal.pone.0270499.s002
DOI:
10.1371/journal.pone.0270499.s003
DOI:
10.1371/journal.pone.0270499.s004
DOI:
10.1371/journal.pone.0270499.s005
DOI:
10.1371/journal.pone.0270499.s006
DOI:
10.1371/journal.pone.0270499.s007
DOI:
10.1371/journal.pone.0270499.s008
DOI:
10.1371/journal.pone.0270499.s009
DOI:
10.1371/journal.pone.0270499.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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