In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2021-12-14), p. e0260850-
Abstract:
Novel strains of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) harboring nucleotide changes (mutations) in the spike gene have emerged and are spreading rapidly. These mutations are associated with SARS-CoV-2 transmissibility, virulence, or resistance to some neutralizing antibodies. Thus, the accurate detection of spike mutants is crucial for controlling SARS-CoV-2 transmission and identifying neutralizing antibody-resistance caused by amino acid changes in the receptor-binding domain. Here, we developed five SARS-CoV-2 spike gene primer pairs (5-SSG primer assay; 69S, 144S, 417S, 484S, and 570S) and verified their ability to detect nine key spike mutations (ΔH69/V70, T95I, G142D, ΔY144, K417T/N, L452R, E484K/Q, N501Y, and H655Y) using a Sanger sequencing-based assay. The 5-SSG primer assay showed 100% specificity and a conservative limit of detection with a median tissue culture infective dose (TCID 50 ) values of 1.4 × 10 2 TCID 50 /mL. The accuracy of the 5-SSG primer assay was confirmed by next generation sequencing. The results of these two approaches showed 100% consistency. Taken together, the ability of the 5-SSG primer assay to accurately detect key SARS-CoV-2 spike mutants is reliable. Thus, it is a useful tool for detecting SARS-CoV-2 spike gene mutants in a clinical setting, thereby helping to improve the management of patients with COVID-19.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0260850
DOI:
10.1371/journal.pone.0260850.g001
DOI:
10.1371/journal.pone.0260850.g002
DOI:
10.1371/journal.pone.0260850.t001
DOI:
10.1371/journal.pone.0260850.t002
DOI:
10.1371/journal.pone.0260850.t003
DOI:
10.1371/journal.pone.0260850.t004
DOI:
10.1371/journal.pone.0260850.t005
DOI:
10.1371/journal.pone.0260850.s001
DOI:
10.1371/journal.pone.0260850.s002
DOI:
10.1371/journal.pone.0260850.s003
DOI:
10.1371/journal.pone.0260850.s004
DOI:
10.1371/journal.pone.0260850.s005
DOI:
10.1371/journal.pone.0260850.s006
DOI:
10.1371/journal.pone.0260850.r001
DOI:
10.1371/journal.pone.0260850.r002
DOI:
10.1371/journal.pone.0260850.r003
DOI:
10.1371/journal.pone.0260850.r004
DOI:
10.1371/journal.pone.0260850.r005
DOI:
10.1371/journal.pone.0260850.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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