In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2022-1-24), p. e0262194-
Abstract:
Tomato zonate spot virus (TZSV), a member of the genus orthotospovirus , causes severe damage to vegetables and ornamental crops in southwest China. The NSs protein is an RNA silencing suppressor in various orthotospovirus like TZSV, but its mechanism and role in virus infection are poorly understood. Here, we observed that an NSs-GFP fusion protein was transiently expressed on the plasma membrane and Golgi bodies in Nicotiana benthamiana plants. The TZSV NSs gene was silenced and infiltrated into N . benthamiana and N . tabacum cv. K326. RT-qPCR and Indirect enzyme-linked immunosorbent assay (ID-ELISA) showed that the transcription and the protein expression of the NSs gene were inhibited by more than 90.00%, and the symptoms on silenced plants were alleviated. We also found that the expression of the Zingipain-2-like gene significantly decreased when the NSs gene was silenced, resulting in co-localization of the NSs-GFP and the Zingipain-2-like-mCherry fusion protein. The findings of this study provide new insights into the mechanism of silencing suppression by NSs, as well as its effect on systemic virus infection, and also support the theory of disease resistance breeding and control and prevention of TZSV in the field.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0262194
DOI:
10.1371/journal.pone.0262194.g001
DOI:
10.1371/journal.pone.0262194.g002
DOI:
10.1371/journal.pone.0262194.g003
DOI:
10.1371/journal.pone.0262194.g004
DOI:
10.1371/journal.pone.0262194.t001
DOI:
10.1371/journal.pone.0262194.s001
DOI:
10.1371/journal.pone.0262194.s002
DOI:
10.1371/journal.pone.0262194.s003
DOI:
10.1371/journal.pone.0262194.s004
DOI:
10.1371/journal.pone.0262194.s005
DOI:
10.1371/journal.pone.0262194.s006
DOI:
10.1371/journal.pone.0262194.r001
DOI:
10.1371/journal.pone.0262194.r002
DOI:
10.1371/journal.pone.0262194.r003
DOI:
10.1371/journal.pone.0262194.r004
DOI:
10.1371/journal.pone.0262194.r005
DOI:
10.1371/journal.pone.0262194.r006
DOI:
10.1371/journal.pone.0262194.r007
DOI:
10.1371/journal.pone.0262194.r008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
Permalink