In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 12 ( 2021-12-16), p. e1010092-
Abstract:
The development of safe and effective vaccines to prevent SARS-CoV-2 infections remains an urgent priority worldwide. We have used a recombinant vesicular stomatitis virus (rVSV)-based prime-boost immunization strategy to develop an effective COVID-19 vaccine candidate. We have constructed VSV genomes carrying exogenous genes resulting in the production of avirulent rVSV carrying the full-length spike protein (S F ), the S1 subunit, or the receptor-binding domain (RBD) plus envelope (E) protein of SARS-CoV-2. Adding the honeybee melittin signal peptide (msp) to the N-terminus enhanced the protein expression, and adding the VSV G protein transmembrane domain and the cytoplasmic tail (Gtc) enhanced protein incorporation into pseudotype VSV. All rVSVs expressed three different forms of SARS-CoV-2 spike proteins, but chimeras with VSV-Gtc demonstrated the highest rVSV-associated expression. In immunized mice, rVSV with chimeric S protein-Gtc derivatives induced the highest level of potent neutralizing antibodies and T cell responses, and rVSV harboring the full-length msp-S F -Gtc proved to be the superior immunogen. More importantly, rVSV-msp-S F -Gtc vaccinated animals were completely protected from a subsequent SARS-CoV-2 challenge. Overall, we have developed an efficient strategy to induce a protective response in SARS-CoV-2 challenged immunized mice. Vaccination with our rVSV-based vector may be an effective solution in the global fight against COVID-19.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010092
DOI:
10.1371/journal.ppat.1010092.g001
DOI:
10.1371/journal.ppat.1010092.g002
DOI:
10.1371/journal.ppat.1010092.g003
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10.1371/journal.ppat.1010092.g004
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10.1371/journal.ppat.1010092.g005
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10.1371/journal.ppat.1010092.g006
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10.1371/journal.ppat.1010092.g007
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10.1371/journal.ppat.1010092.g008
DOI:
10.1371/journal.ppat.1010092.g009
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10.1371/journal.ppat.1010092.g010
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10.1371/journal.ppat.1010092.g011
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10.1371/journal.ppat.1010092.t001
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10.1371/journal.ppat.1010092.s001
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10.1371/journal.ppat.1010092.s002
DOI:
10.1371/journal.ppat.1010092.s003
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10.1371/journal.ppat.1010092.s004
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10.1371/journal.ppat.1010092.s005
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10.1371/journal.ppat.1010092.s006
DOI:
10.1371/journal.ppat.1010092.s007
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10.1371/journal.ppat.1010092.s008
DOI:
10.1371/journal.ppat.1010092.s009
DOI:
10.1371/journal.ppat.1010092.s010
DOI:
10.1371/journal.ppat.1010092.s011
DOI:
10.1371/journal.ppat.1010092.s012
DOI:
10.1371/journal.ppat.1010092.s013
DOI:
10.1371/journal.ppat.1010092.s014
DOI:
10.1371/journal.ppat.1010092.s015
DOI:
10.1371/journal.ppat.1010092.s016
DOI:
10.1371/journal.ppat.1010092.s017
DOI:
10.1371/journal.ppat.1010092.s018
DOI:
10.1371/journal.ppat.1010092.s019
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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