In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 1 ( 2021-1-19), p. e0239171-
Kurzfassung:
rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L . infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L . infantum .
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0239171
DOI:
10.1371/journal.pone.0239171.g001
DOI:
10.1371/journal.pone.0239171.g002
DOI:
10.1371/journal.pone.0239171.g003
DOI:
10.1371/journal.pone.0239171.g004
DOI:
10.1371/journal.pone.0239171.g005
DOI:
10.1371/journal.pone.0239171.s001
DOI:
10.1371/journal.pone.0239171.r001
DOI:
10.1371/journal.pone.0239171.r002
DOI:
10.1371/journal.pone.0239171.r003
DOI:
10.1371/journal.pone.0239171.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2021
ZDB Id:
2267670-3
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