In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 6 ( 2021-6-24), p. e0253541-
Abstract:
We assessed the applicability of next-generation sequencing (NGS)-based IGH / IGK clonality testing and analyzed the repertoire of immunoglobulin heavy chain ( IGH ) or immunoglobulin kappa light chain ( IGK ) gene usage in Korean patients with multiple myeloma (MM) for the first time. Methods Fifty-nine bone marrow samples from 57 Korean patients with MM were analyzed, and NGS-based clonality testing that targeted the IGH and IGK genes was performed using IGH FR1 and IGK primer sets. Results Clonal IGH and IGK rearrangements were observed in 74.2% and 67.7% of samples from Korean patients with kappa-restricted MM, respectively (90.3% had one or both), and in 60.7% and 95.5% of samples from those with lambda-restricted MM, respectively (85.7% had one or both). In total, 88.1% of samples from Koreans with MM had clonal IGH and/or IGK rearrangement. Clonal rearrangement was not significantly associated with the bone marrow plasma cells as a proportion of all BM lymphoid cells. IGHV3-9 (11.63%) and IGHV4-31 (9.30%) were the most frequently reported IGHV genes and were more common in Koreans with MM than in Western counterparts. IGHD3-10 and IGHD3-3 (13.95% each) were the most frequent IGHD genes; IGHD3-3 was more common in Koreans with MM. No IGK rearrangement was particularly prevalent, but single IGKV-J rearrangements were less common in Koreans with kappa-restricted MM than in Western counterparts. IGKV4-1 was less frequent in Koreans regardless of light chain type. Otherwise, the usages of the IGH V, D, and J genes and of the IGK gene were like those observed in previous Western studies. Conclusion NGS-based IGH / IGK clonality testing ought to be applicable to most Koreans with MM. The overrepresentation of IGHV3-9 , IGHV4-31 , and IGHD3-3 along with the underrepresentation of IGKV4-1 and the differences in IGK gene rearrangement types suggest the existence of ethnicity-specific variations in this disease.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0253541
DOI:
10.1371/journal.pone.0253541.t001
DOI:
10.1371/journal.pone.0253541.t002
DOI:
10.1371/journal.pone.0253541.t003
DOI:
10.1371/journal.pone.0253541.t004
DOI:
10.1371/journal.pone.0253541.t005
DOI:
10.1371/journal.pone.0253541.t006
DOI:
10.1371/journal.pone.0253541.s001
DOI:
10.1371/journal.pone.0253541.s002
DOI:
10.1371/journal.pone.0253541.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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